农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
11期
89-91
,共3页
蓝玉甜%刘世勇%罗玉婷%黄岚%李柱林%韦小路
藍玉甜%劉世勇%囉玉婷%黃嵐%李柱林%韋小路
람옥첨%류세용%라옥정%황람%리주림%위소로
鼓槌石斛%无菌播种%幼苗培养%快速繁殖
鼓槌石斛%無菌播種%幼苗培養%快速繁殖
고퇴석곡%무균파충%유묘배양%쾌속번식
Dendrobium chrysotoxum%Sterile germination%Plantlet culture%Rapid propagation
[目的]利用鼓槌石斛蒴果,通过无菌播种萌发发育成苗及小苗快繁技术试验研究,从而获得大量种苗.[方法]用鼓槌石斛种子通过无菌播种获得大量原球茎,分别转接到4组不同的诱导芽培养基上,筛选出鼓槌石斛最适合小苗分化和成长的培养基,以其设置以N6为基本培养基,添加浓度均为1.0、1.5、2.0 mg/ L 的NAA和IAA,筛选最适合鼓槌石斛小苗生根壮苗培养基.[结果]将鼓槌石斛种子在MS+6-BA 1 mg/L+10%香蕉汁+ 20 g/L蔗糖+6 g/L琼脂+1 g/L AC培养基上进行培养种子,种子萌发率达90%以上;最利于原球茎分化的培养基为N6+ NAA 2 mg/L + BA 0.5 mg/L+10%香蕉汁+ 20 g/L蔗糖 + 0.5 g/L牛肉蛋白胨+ 5.8 g/L琼脂+0.5 g/L AC,培养出的苗整齐度高,均匀;最佳生根壮苗培养基为N6+NAA 1.5 mg/ L+10%香蕉汁+ 20 g/L蔗糖 + 5.8 g/L琼脂+1 g/L AC,苗生根数多、粗壮、整齐,叶浓绿.[结论]该研究结果为鼓槌石斛快速繁育技术提供了参考.
[目的]利用鼓槌石斛蒴果,通過無菌播種萌髮髮育成苗及小苗快繁技術試驗研究,從而穫得大量種苗.[方法]用鼓槌石斛種子通過無菌播種穫得大量原毬莖,分彆轉接到4組不同的誘導芽培養基上,篩選齣鼓槌石斛最適閤小苗分化和成長的培養基,以其設置以N6為基本培養基,添加濃度均為1.0、1.5、2.0 mg/ L 的NAA和IAA,篩選最適閤鼓槌石斛小苗生根壯苗培養基.[結果]將鼓槌石斛種子在MS+6-BA 1 mg/L+10%香蕉汁+ 20 g/L蔗糖+6 g/L瓊脂+1 g/L AC培養基上進行培養種子,種子萌髮率達90%以上;最利于原毬莖分化的培養基為N6+ NAA 2 mg/L + BA 0.5 mg/L+10%香蕉汁+ 20 g/L蔗糖 + 0.5 g/L牛肉蛋白胨+ 5.8 g/L瓊脂+0.5 g/L AC,培養齣的苗整齊度高,均勻;最佳生根壯苗培養基為N6+NAA 1.5 mg/ L+10%香蕉汁+ 20 g/L蔗糖 + 5.8 g/L瓊脂+1 g/L AC,苗生根數多、粗壯、整齊,葉濃綠.[結論]該研究結果為鼓槌石斛快速繁育技術提供瞭參攷.
[목적]이용고퇴석곡삭과,통과무균파충맹발발육성묘급소묘쾌번기술시험연구,종이획득대량충묘.[방법]용고퇴석곡충자통과무균파충획득대량원구경,분별전접도4조불동적유도아배양기상,사선출고퇴석곡최괄합소묘분화화성장적배양기,이기설치이N6위기본배양기,첨가농도균위1.0、1.5、2.0 mg/ L 적NAA화IAA,사선최괄합고퇴석곡소묘생근장묘배양기.[결과]장고퇴석곡충자재MS+6-BA 1 mg/L+10%향초즙+ 20 g/L자당+6 g/L경지+1 g/L AC배양기상진행배양충자,충자맹발솔체90%이상;최리우원구경분화적배양기위N6+ NAA 2 mg/L + BA 0.5 mg/L+10%향초즙+ 20 g/L자당 + 0.5 g/L우육단백동+ 5.8 g/L경지+0.5 g/L AC,배양출적묘정제도고,균균;최가생근장묘배양기위N6+NAA 1.5 mg/ L+10%향초즙+ 20 g/L자당 + 5.8 g/L경지+1 g/L AC,묘생근수다、조장、정제,협농록.[결론]해연구결과위고퇴석곡쾌속번육기술제공료삼고.
[Objective] This study was to produce plant seeds on a large scale via sterile germination of the capsules of Dendrobium chrysotoxum and rapid propagation technique.[Method] A large amount of protocorm-like bodies produced from the sterile germination of D.chrysotoxum capsules, were transferred to four different kinds of bud induction media to obtain the optimal media suitable for plantlet differentiation and growth; and then with N6 as basic medium, 1.0, 1.5 and 2.0 mg/L of NAA and IAA were tested to obtain the optimal media suitable for rooting.[Results] On the medium of MS appended with 1 mg/L 6-BA +10% banana puree + 20 g/L sucrose +6 g/L agar+1 g/L AC, seed germination rate was up to 90%.The optimal medium for differentiation of D.chrysotoxum protocorm-like bodies was N6+ 2 mg/L NAA + 0.5 mg/L 6-BA +10% banana puree + 20 g/L sucrose + 0.5 g/L peptone + 5.8 g/L agar +0.5 g/L AC, grown from which the plantlets were even and orderly in height; and the optimal medium for rooting was N6+1.5 mg/ L NAA +10% banana puree + 20 g/L sucrose + 5.8 g/L agar +1 g/L AC, grown from which the plantlets developed more, robust and orderly roots, and their leaves were in dark green color.[Conclusion] Our results provided reference for the rapid propagation of D.chrysotoxum.