CAJ | 학술논문

目的探讨淋巴细胞趋化因子(lymphotactin,Ltn)与大鼠白色念珠菌肺炎发生发展过程的相关性.方法选用清洁级健康雄性SD大鼠(150～180 g,鼠龄30～40 d)80只,随机分为模型组与对照组,每组40只.实验组从气管注入白色念珠菌菌液制备白色念珠菌肺炎模型,分别在接种完成后1 d、3 d、7 d、11 d处死大鼠(各组10只),取心室血,采用双抗体夹心ABC-酶联免疫吸附试验法测定血清Ltn、γ-干扰素(IFN-γ)、白介素2(IL-2)水平,取肺组织行组织病理检查及病原体培养计数,应用SPSS 13.0统计软件对数据结果进行处理.结果模型1 d、3 d、7 d、11 d组均见明显炎症反应,Szapiel评分分别为(1.70±0.48)分、(3.60±0.70)分、(2.90±0.57)分、(1.30±0.52)分,菌体负荷数分别为(3.50±1.07)×106 du/ml、(4.60±1.08)×106cfu/ml、(1.30±0.48)×106cfu/ml、(0.10±0.32)×106cfu/ml.模型1 d、3 d、7 d组Ltn、IFN-γ、IL-2水平均高于对照组(P<0.05),模型组Ltn的消长与炎性程度、菌落数、IFN-γ、IL-2水平呈正相关(γ=0.816、0.647、0.846、0.823,P值均<0.05).结论 Ltn参与了真菌感染过程.与IFN-γ、IL-2在消除病原体方面起着相互协同的积极作用.
목적 탐토림파세포추화인자(lymphotactin,Ltn)여대서백색념주균폐염발생발전과정적상관성.방법 선용청길급건강웅성SD대서(150～180 g,서령30～40 d)80지,수궤분위모형조여대조조,매조40지.실험조종기관주입백색념주균균액제비백색념주균폐염모형,분별재접충완성후1 d、3 d、7 d、11 d처사대서(각조10지),취심실혈,채용쌍항체협심ABC-매련면역흡부시험법측정혈청Ltn、γ-간우소(IFN-γ)、백개소2(IL-2)수평,취폐조직행조직병리검사급병원체배양계수,응용SPSS 13.0통계연건대수거결과진행처리.결과 모형1 d、3 d、7 d、11 d조균견명현염증반응,Szapiel평분분별위(1.70±0.48)분、(3.60±0.70)분、(2.90±0.57)분、(1.30±0.52)분,균체부하수분별위(3.50±1.07)×106 du/ml、(4.60±1.08)×106cfu/ml、(1.30±0.48)×106cfu/ml、(0.10±0.32)×106cfu/ml.모형1 d、3 d、7 d조Ltn、IFN-γ、IL-2수평균고우대조조(P<0.05),모형조Ltn적소장여염성정도、균락수、IFN-γ、IL-2수평정정상관(γ=0.816、0.647、0.846、0.823,P치균<0.05).결론 Ltn삼여료진균감염과정.여IFN-γ、IL-2재소제병원체방면기착상호협동적적겁작용.
Objective To investigate the correlation between lymphotactin(Ltn)and pulmonary candidiasis albicans in rats.Methods Eighty male SD rats(150-180 g,30-40 days of age)were randomly divided into control group and model group(n = 40).In model group,pulmonary candidiasis albicans model was prepared by the injection of Candida albicans from the trachea.Experimental animals were sacrificed at 1 d,3 d,7 d,11 d after vaccination,and ventricle blood was taken.The levels of Ltn,interferon-γ(IFN-γ),interleukin-2(IL-2)in serum were measured by double-antibody sandwich ABC-enzyme linked immunosorbent assay.Lung tissues were taken for tissue pathology examination and pathogen count training.The data were analyzed by SPSS 13.0.Results Inflammatory reaction was observed in model 1 d,3 d,7 d,11 d groups.Szapiel scores were 1.70 ±0.48,3.60±0.70,2.90±0.57,1.30± 0.52,respectively.The numbers of bacterial load were(3.50±1.07)× 106 cfu/ml,(4.60± 1.08)× 106 cfu/ml,(1.30± 0.48)× 106 cfu/ml,(0.10± 0.32)× 106 cfu/ml,respectively.Compared with control group,the levels of Ltn,IFN-γ and IL-2 increased in model 1 d,3 d,7 d,11 d groups(P <0.05).There was positive correlation between Ltn level and the degree of inflammation,the number of coenobium,the levels of IFN-γ and IL-2 in model group(r = 0.816,0.647,0.846,0.823,all P <0.05).Conclusions Ltn might participate in the course of fungal infection,and play a positively synergistical role with IFN-γ,IL-2 on the elimination of pathogens.Objective To investigate the correlation between lymphotactin(Ltn)and pulmonary candidiasis albicans in rats.Methods Eighty male SD rats(150-180 g,30-40 days of age)were randomly divided into control group and model group(n = 40).In model group,pulmonary candidiasis albicans model was prepared by the injection of Candida albicans from the trachea.Experimental animals were sacrificed at 1 d,3 d,7 d,11 d after vaccination,and ventricle blood was taken.The levels of Ltn,interferon-γ(IFN-γ),interleukin-2(IL-2)in serum were measured by double-antibody sandwich ABC-enzyme linked immunosorbent assay.Lung tissues were taken for tissue pathology examination and pathogen count training.The data were analyzed by SPSS 13.0.Results Inflammatory reaction was observed in model 1 d,3 d,7 d,11 d groups.Szapiel scores were 1.70 ±0.48,3.60±0.70,2.90±0.57,1.30± 0.52,respectively.The numbers of bacterial load were(3.50±1.07)× 106 cfu/ml,(4.60± 1.08)× 106 cfu/ml,(1.30± 0.48)× 106 cfu/ml,(0.10± 0.32)× 106 cfu/ml,respectively.Compared with control group,the levels of Ltn,IFN-γ and IL-2 increased in model 1 d,3 d,7 d,11 d groups(P <0.05).There was positive correlation between Ltn level and the degree of inflammation,the number of coenobium,the levels of IFN-γ and IL-2 in model group(r = 0.816,0.647,0.846,0.823,all P <0.05).Conclusions Ltn might participate in the course of fungal infection,and play a positively synergistical role with IFN-γ,IL-2 on the elimination of pathogens.