CAJ | 학술논문

目的探讨血管紧张素-(1-7)[Ang-(1-7)]时转化生长因子-β1(TGF-β1)诱导大鼠系膜细胞(GMC)增殖和细胞外基质分泌的影响.方法选择对数生长期GMC,分为对照组、Ang-(1-7)组、TGF-β1组、TGF-β1+Ang-(1-7)组,采用WST-1检测系膜细胞增殖,RT-PCR法和放射免疫法检测细胞外基质层粘蛋白(LN)、Ⅳ型胶原(COL Ⅳ)的表达.结果与对照组相比,Ang-(1-7)组细胞数目和细胞外基质减少(P<0.05);与TGF-β1组相比,Ang-(1-7)可抑制TGF-β1诱导的系膜细胞增殖、使细胞内LN mR-NA、COLⅣmRNA表达量明显下调、同时导致上清液中细胞外基质LN、COLⅣ含量显著减少(P<0.05).结论 Ang-(1-7)能抑制基础和TGF-β1诱导的GMC细胞增殖及细胞外基质的分泌,可能在抑制肾脏纤维化过程中发挥作用.
목적 탐토혈관긴장소-(1-7)[Ang-(1-7)]시전화생장인자-β1(TGF-β1)유도대서계막세포(GMC)증식화세포외기질분비적영향.방법 선택대수생장기GMC,분위대조조、Ang-(1-7)조、TGF-β1조、TGF-β1+Ang-(1-7)조,채용WST-1검측계막세포증식,RT-PCR법화방사면역법검측세포외기질층점단백(LN)、Ⅳ형효원(COL Ⅳ)적표체.결과 여대조조상비,Ang-(1-7)조세포수목화세포외기질감소(P<0.05);여TGF-β1조상비,Ang-(1-7)가억제TGF-β1유도적계막세포증식、사세포내LN mR-NA、COLⅣmRNA표체량명현하조、동시도치상청액중세포외기질LN、COLⅣ함량현저감소(P<0.05).결론 Ang-(1-7)능억제기출화TGF-β1유도적GMC세포증식급세포외기질적분비,가능재억제신장섬유화과정중발휘작용.
Objective To explore the effect of angiotensin-(1-7) [Ang-(1-7)] on proliferation and secretion in cultured rat's glo-merular mesangial cells(GMC) induced by transforming growth factor-β1 (TGF-β1). Methods GMC in logarithmic growth phase were in-cubated, and then were divided into 4 groups: control, Ang-(1-7), TGF-β1,and TGF-β1 + Ang-(1-7) group. Cell numbers of rat's GMC were detected by WST-1. Laminin(LN) and collagenlV (ColⅣ) mRNA expressions in GMC were analyzed by RT-PCR. The secretion of LN and ColⅣ in culture medium of rat's GMC was measured by radioimmunoassay. Results Ang-(1-7) significantly inhibited basal and TGF-β1-induced proliferation of cultured glomerular mesangial cells. Ang-(1-7) significantly down-regulated LN and Col Ⅳ mRNA expressions in glomerular mesangial cells and also inhibited the secretion of LN and ColⅣ induced by TGF-β1(P <0. 05). Conclusions Ang-(1-7) can inhibit basal and TGF-β1-induced proliferation, and inhibit ECM secretion of cultured rats glomerular mesangial cells.