中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2011年
1期
28-33
,共6页
串联质谱%氨基酸%性别%年龄
串聯質譜%氨基痠%性彆%年齡
천련질보%안기산%성별%년령
Tandem mass spectrometry%Amino acid%Sex%Age
目的 调查0～15岁儿童血中氨基酸浓度的变化情况,为评价氨基酸营养状态和诊断氨基酸代谢病提供帮助.方法 以1900名0～15岁儿童为研究对象,采用非衍生法前处理滤纸血片,串联质谱测定11种氨基酸浓度.结果 血缬氨酸、亮氨酸、苯丙氨酸、甘氨酸、酪氨酸、脯氨酸、鸟氨酸和丙氨酸浓度在出生后开始逐渐降低,4～6个月时最低,此后逐渐升高,7个月～1岁后相对稳定;血蛋氨酸、精氨酸浓度在出生后最低,1～3个月时最高,此后开始下降,4～6个月后水平稳定;血丙氨酸和甘氨酸浓度分别在9岁女孩和11岁男孩中达到第2高峰.鸟氨酸/精氨酸、瓜氨酸/精氨酸和鸟氨酸/瓜氨酸的比值出生时较高,此后逐渐降低,3个月～1岁后维持稳定;苯丙氨酸/酪氨酸比值在出生时最低,以后逐渐升高,6个月～1岁后维持稳定.7个月～15岁年龄组的血氨基酸水平与1 d～1个月年龄组和/或2～6个月年龄组间差异均有统计学意义(P＜0.05或P＜0.01).1 d～1个月年龄组和全年龄组女性氨基酸浓度均明显高于男性(P＜0.05或P＜0.01).结论 1岁以内血中氨基酸浓度和构成比变化较大,在评价氨基酸营养状态和诊断氨基酸代谢病时要考虑年龄因素.
目的 調查0～15歲兒童血中氨基痠濃度的變化情況,為評價氨基痠營養狀態和診斷氨基痠代謝病提供幫助.方法 以1900名0～15歲兒童為研究對象,採用非衍生法前處理濾紙血片,串聯質譜測定11種氨基痠濃度.結果 血纈氨痠、亮氨痠、苯丙氨痠、甘氨痠、酪氨痠、脯氨痠、鳥氨痠和丙氨痠濃度在齣生後開始逐漸降低,4～6箇月時最低,此後逐漸升高,7箇月～1歲後相對穩定;血蛋氨痠、精氨痠濃度在齣生後最低,1～3箇月時最高,此後開始下降,4～6箇月後水平穩定;血丙氨痠和甘氨痠濃度分彆在9歲女孩和11歲男孩中達到第2高峰.鳥氨痠/精氨痠、瓜氨痠/精氨痠和鳥氨痠/瓜氨痠的比值齣生時較高,此後逐漸降低,3箇月～1歲後維持穩定;苯丙氨痠/酪氨痠比值在齣生時最低,以後逐漸升高,6箇月～1歲後維持穩定.7箇月～15歲年齡組的血氨基痠水平與1 d～1箇月年齡組和/或2～6箇月年齡組間差異均有統計學意義(P＜0.05或P＜0.01).1 d～1箇月年齡組和全年齡組女性氨基痠濃度均明顯高于男性(P＜0.05或P＜0.01).結論 1歲以內血中氨基痠濃度和構成比變化較大,在評價氨基痠營養狀態和診斷氨基痠代謝病時要攷慮年齡因素.
목적 조사0～15세인동혈중안기산농도적변화정황,위평개안기산영양상태화진단안기산대사병제공방조.방법 이1900명0～15세인동위연구대상,채용비연생법전처리려지혈편,천련질보측정11충안기산농도.결과 혈힐안산、량안산、분병안산、감안산、락안산、포안산、조안산화병안산농도재출생후개시축점강저,4～6개월시최저,차후축점승고,7개월～1세후상대은정;혈단안산、정안산농도재출생후최저,1～3개월시최고,차후개시하강,4～6개월후수평은정;혈병안산화감안산농도분별재9세녀해화11세남해중체도제2고봉.조안산/정안산、과안산/정안산화조안산/과안산적비치출생시교고,차후축점강저,3개월～1세후유지은정;분병안산/락안산비치재출생시최저,이후축점승고,6개월～1세후유지은정.7개월～15세년령조적혈안기산수평여1 d～1개월년령조화/혹2～6개월년령조간차이균유통계학의의(P＜0.05혹P＜0.01).1 d～1개월년령조화전년령조녀성안기산농도균명현고우남성(P＜0.05혹P＜0.01).결론 1세이내혈중안기산농도화구성비변화교대,재평개안기산영양상태화진단안기산대사병시요고필년령인소.
Objective To investigate the blood levels of amino acids in children aged 0-15 year, with an attempt to provide evidence for evaluating amino acid status and diagnosing metabolic diseases of amino acid.Methods The blood levels of eleven amino acids in 1900 children aged 0-15 years were determined by tandem mass spectrometry (MS/MS). Results The blood levels of leucine & isoleucine, valine, phenylalanine tyrosine,glycine, proline, ornithine, and alanine gradually decreased after birth, reaching the lowest levels at the ages of 4-6 months, and then gradually increased, reaching the normal range at the ages of 7 months-1 year. The blood levels of alanine and glycine reach the second peaks on the ages of 9 years in girls and on 11 years in boys. The blood levels of methionine and arginine were lowest in the first week of age, became highest in 1-3 months, decreased to the normal ranges after 4-6 months, and kept the level afterwards. The ratios between prosomatic amino acid and productive amino acid, between ornithine and arginine, between citrulline and arginine, and between ornithin and citrulline were highest in the first week of age and decreased to normal values after 3 to 12 months. The concentrations of amino acids in group of 7 months-15 years were significantly different from the group of 1 day-1 month and group of 2-6 months (P ＜0. 05 or P ＜0. 01 ). The concentrations of amino acids were significantly higher in females than in males in the group of 1 day-1 month and in all age groups (P ＜0. 05 or P ＜0. 01 ) . Conclusions The concentrations and profiles of amino acids change remarkably during the first year of age. Age should be carefully considered when evaluating the nutritional status of amino acid and diagnosing metabolic diseases of amino acids.
