中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
7期
479-482
,共4页
瘢痕,肥大性%激光%毛细血管%成纤维细胞%胶原
瘢痕,肥大性%激光%毛細血管%成纖維細胞%膠原
반흔,비대성%격광%모세혈관%성섬유세포%효원
Cicatrix,hypertrophic%Lasers%Capillaries%Fibroblasts%Collagen
目的 通过建立兔耳增生性瘢痕模型,评价长脉冲1064 nm激光治疗增生性瘢痕的疗效.方法 选用新西兰长耳白兔10只,雌雄各半,体质量2.0～2.5 kg.在所有兔耳腹侧面建立增生性瘢痕模型,每只兔耳4处1.5 cm x 1.5 cm正方形造模.10只兔子共80个创面形成增生性瘢痕74处,将左侧和右侧兔耳增生性瘢痕块分为对照组和治疗组,治疗组应用长脉冲1064 nm激光照射,对照组未予治疗.30 d后观察实验组及对照组瘢痕的颜色、质地;彩色超声测量瘢痕厚度;瘢痕取材,HE染色和CD31免疫组化染色,记数瘢痕成纤维细胞密度和微血管密度;Masson染色观察胶原纤维.结果 激光治疗组较对照组瘢痕颜色变浅,质地变柔软,瘢痕厚度变薄,对照组搬痕的平均厚度为3.089 mill,治疗组为2.137 mm,两组比较,t=5.72,P<0.01.对照组血管密度均值为68.056个/mm2,治疗组为38.333个/mm2,两组比较,t=4.93,P<0.01,治疗组血管密度较对照组明显降低.成纤维细胞数量均值对照组为355.000个/mm2,治疗组为166.940个/mm2,两组比较,t=13.36,P<0.01,治疗组成纤维细胞数量明显减少.Masson染色观察对照组胶原纤维排列紊乱,治疗组胶原纤维排列疏松,规则.结论 长脉冲1064 nm激光可以促进早期增生性瘢痕消退,对瘢痕增生具有抑制作用.
目的 通過建立兔耳增生性瘢痕模型,評價長脈遲1064 nm激光治療增生性瘢痕的療效.方法 選用新西蘭長耳白兔10隻,雌雄各半,體質量2.0～2.5 kg.在所有兔耳腹側麵建立增生性瘢痕模型,每隻兔耳4處1.5 cm x 1.5 cm正方形造模.10隻兔子共80箇創麵形成增生性瘢痕74處,將左側和右側兔耳增生性瘢痕塊分為對照組和治療組,治療組應用長脈遲1064 nm激光照射,對照組未予治療.30 d後觀察實驗組及對照組瘢痕的顏色、質地;綵色超聲測量瘢痕厚度;瘢痕取材,HE染色和CD31免疫組化染色,記數瘢痕成纖維細胞密度和微血管密度;Masson染色觀察膠原纖維.結果 激光治療組較對照組瘢痕顏色變淺,質地變柔軟,瘢痕厚度變薄,對照組搬痕的平均厚度為3.089 mill,治療組為2.137 mm,兩組比較,t=5.72,P<0.01.對照組血管密度均值為68.056箇/mm2,治療組為38.333箇/mm2,兩組比較,t=4.93,P<0.01,治療組血管密度較對照組明顯降低.成纖維細胞數量均值對照組為355.000箇/mm2,治療組為166.940箇/mm2,兩組比較,t=13.36,P<0.01,治療組成纖維細胞數量明顯減少.Masson染色觀察對照組膠原纖維排列紊亂,治療組膠原纖維排列疏鬆,規則.結論 長脈遲1064 nm激光可以促進早期增生性瘢痕消退,對瘢痕增生具有抑製作用.
목적 통과건립토이증생성반흔모형,평개장맥충1064 nm격광치료증생성반흔적료효.방법 선용신서란장이백토10지,자웅각반,체질량2.0～2.5 kg.재소유토이복측면건립증생성반흔모형,매지토이4처1.5 cm x 1.5 cm정방형조모.10지토자공80개창면형성증생성반흔74처,장좌측화우측토이증생성반흔괴분위대조조화치료조,치료조응용장맥충1064 nm격광조사,대조조미여치료.30 d후관찰실험조급대조조반흔적안색、질지;채색초성측량반흔후도;반흔취재,HE염색화CD31면역조화염색,기수반흔성섬유세포밀도화미혈관밀도;Masson염색관찰효원섬유.결과 격광치료조교대조조반흔안색변천,질지변유연,반흔후도변박,대조조반흔적평균후도위3.089 mill,치료조위2.137 mm,량조비교,t=5.72,P<0.01.대조조혈관밀도균치위68.056개/mm2,치료조위38.333개/mm2,량조비교,t=4.93,P<0.01,치료조혈관밀도교대조조명현강저.성섬유세포수량균치대조조위355.000개/mm2,치료조위166.940개/mm2,량조비교,t=13.36,P<0.01,치료조성섬유세포수량명현감소.Masson염색관찰대조조효원섬유배렬문란,치료조효원섬유배렬소송,규칙.결론 장맥충1064 nm격광가이촉진조기증생성반흔소퇴,대반흔증생구유억제작용.
Objective To evaluate the therapeutic effect of long-pulsed Nd:YAG 1064 laser on hyperplastic scars by using a rabbit ear model.Methods Five female and five male New Zealand long-ear white rabbits weighting 2.0-2.5 kg were used in this experiment.Four square full-thickness skin wounds sized 1.5 cm x1.5 cm were created on the ventral surface of each ear to develop a model of hyperplastic scar.Finally,a total of 74 hyperplastic scars developed on the 80 wounds,and the scars on the left and right ears served as the control (unirradiated) and treatment (irradiated with long-pulsed 1064 nm Nd:YAG laser) group,respectively.After 30 days of irradiation,the color and texture of scars were observed and the scar thickness was measured by color Doppler ultrasonogTaphy.Then,the scars were harvested followed by the analysis of density of fibroblasts and microvessels as well as the changes in collagen fibers in scars by HE staining,CD31 staining and Masson staining,respectively.Results A decrease was observed in the color,hardness and thickness of scars in the irradiated ears compared with the unirradiated ears.The average thickness of scars,microvessel density and fibroblast density in scars were significantly lower in the treatment group than in the control group(2.137vs.3.089 am,t=5.72,P<0.01;38.333/mm2vs.68.056/mm2,t=4.93,P<0.01;166.940/mm2vs.355.000/mm2,t=13.36.P<0.01).Masson staining revealed a disorganized arrangement of collagen fibers in the control group but a sparse and regular alignment in the treatment group.Conclusion Long-pulsed 1064 nm Nd:YAG laser may promote the shrinkage and suppress the hyperplasia of scars.
