中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2011年
9期
599-602
,共4页
缺氧诱导因子-1α%低氧状态%前列腺癌细胞PC-3%增殖%侵袭
缺氧誘導因子-1α%低氧狀態%前列腺癌細胞PC-3%增殖%侵襲
결양유도인자-1α%저양상태%전렬선암세포PC-3%증식%침습
Hypoxia-inducible factor-1α%Hypoxic%Prostate cancer PC-3%Proliferation%Invasion
目的 研究缺氧诱导因子-1α(HIF-1α)对低氧状态下前列腺癌PC-3细胞株增殖及侵袭的影响。 方法 利用转染试剂Fermentas将人HIF-1α重组表达质粒pcDNA3.1-HIF-1α转染PC-3细胞株后，低氧环境培养，采用G418筛选，建立稳定表达HIF-1α基因的细胞株，分别命名为pcD-NA3.1-HIF-1 α-PC-3、pcDNA3.1-PC-3及PC-3组。采用RT-PCR和蛋白质印迹法检测3组细胞HIF-1α mRNA和蛋白的表达情况；噻唑盐法测定细胞生长；transwell小室检测侵袭能力。 结果 与pcDNA3.1-PC-3组和PC-3组相比，pcDNA3.1-HIF-1 α-PC-3组细胞内HIF-1α mRNA条带增强不明显，pcDNA3.1-HIF-1α-PC-3组细胞内HIF-1α蛋白的条带明显增强，HIF-1α过表达的PC-3细胞增殖速度明显增快，侵袭细胞数明显增多。 结论 HIF-1α过表达对PC-3细胞株的增殖及侵袭具有促进作用。
目的 研究缺氧誘導因子-1α(HIF-1α)對低氧狀態下前列腺癌PC-3細胞株增殖及侵襲的影響。 方法 利用轉染試劑Fermentas將人HIF-1α重組錶達質粒pcDNA3.1-HIF-1α轉染PC-3細胞株後，低氧環境培養，採用G418篩選，建立穩定錶達HIF-1α基因的細胞株，分彆命名為pcD-NA3.1-HIF-1 α-PC-3、pcDNA3.1-PC-3及PC-3組。採用RT-PCR和蛋白質印跡法檢測3組細胞HIF-1α mRNA和蛋白的錶達情況；噻唑鹽法測定細胞生長；transwell小室檢測侵襲能力。 結果 與pcDNA3.1-PC-3組和PC-3組相比，pcDNA3.1-HIF-1 α-PC-3組細胞內HIF-1α mRNA條帶增彊不明顯，pcDNA3.1-HIF-1α-PC-3組細胞內HIF-1α蛋白的條帶明顯增彊，HIF-1α過錶達的PC-3細胞增殖速度明顯增快，侵襲細胞數明顯增多。 結論 HIF-1α過錶達對PC-3細胞株的增殖及侵襲具有促進作用。
목적 연구결양유도인자-1α(HIF-1α)대저양상태하전렬선암PC-3세포주증식급침습적영향。 방법 이용전염시제Fermentas장인HIF-1α중조표체질립pcDNA3.1-HIF-1α전염PC-3세포주후，저양배경배양，채용G418사선，건립은정표체HIF-1α기인적세포주，분별명명위pcD-NA3.1-HIF-1 α-PC-3、pcDNA3.1-PC-3급PC-3조。채용RT-PCR화단백질인적법검측3조세포HIF-1α mRNA화단백적표체정황；새서염법측정세포생장；transwell소실검측침습능력。 결과 여pcDNA3.1-PC-3조화PC-3조상비，pcDNA3.1-HIF-1 α-PC-3조세포내HIF-1α mRNA조대증강불명현，pcDNA3.1-HIF-1α-PC-3조세포내HIF-1α단백적조대명현증강，HIF-1α과표체적PC-3세포증식속도명현증쾌，침습세포수명현증다。 결론 HIF-1α과표체대PC-3세포주적증식급침습구유촉진작용。
Objective WE transfected the recombinant expression plasmid of pcDNA3. 1-HIF-1α into the prostate cancer cells, to research the effect of HIF-1α on proliferation of prostate cancer cell PC-3.Methods We selected a stable expression cell line with G418 were selected by transfection of the recombinant expression plasmid of pcDNA3. 1-HIF-1α into the prostate cancer. The protein and mRNA expression of HIF-1α was assayed by western - blot and RT-PCR. The cells growth curves were described by MTT and the ability of invasion was assayed by Transwell.Results The expression of HIF-1α mRNA was not obviously increased compared to the untransfected prostate cancer cell by RT-PCR, but the expression of HIF-1α protein was up-regulated by western-blot after the recombinant expression plasmid transfected into PC-3. The ability of cell proliferation and invasion was significantly enhanced by MTT and Transwell assays.Conclusion The stable expression cell model of HIF-1α was successfully constructed, which enhanced the proliferation and invasion of prostate cancer cell PC-3.
