中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
6期
1056-1058
,共3页
詹以安%王共先%汪泱%胡红林%傅斌
詹以安%王共先%汪泱%鬍紅林%傅斌
첨이안%왕공선%왕앙%호홍림%부빈
前列腺癌%β干扰素%间充质干细胞%基因治疗
前列腺癌%β榦擾素%間充質榦細胞%基因治療
전렬선암%β간우소%간충질간세포%기인치료
Prostate carcinoma%Interferon beta%Mesenchymal stem cell%Gene therapy
目的 观察人β干扰素(IFN-β)基因转染的人骨髓间充质干细胞(hMSCs)对前列腺癌细胞株PC-3增殖及凋亡的影响.方法 脂质体介导入IFN-β基因转染的hMSCs( IFN-β-h MSCs)与PC-3体外共培养,共培养后第1、3、5天对PC-3进行锥虫蓝染色,计数存活细胞数目.流式细胞仪分析PC-3细胞凋亡.结果 IFN-β-hMSCs可成功分泌表达IFN-β.hMSCs、空质粒转染hMSCs、IFN-β-hMSCs组与PC-3在共培养后第5天,PC-3存活细胞计数分别为(10.81±0.33)×105、(9.90±0.41) ×105、(4.63±0.32)× 105个,PC-3存活细胞差异有统计学意义(P＜0.01)；流式细胞仪检测示实验组PC-3凋亡率为(22.79±2.71)％,较各对照组增加.结论 IFN-β-hMSCs能成功分泌表达IFN-β,体外共培养可有效抑制PC-3增殖,促进其凋亡.
目的 觀察人β榦擾素(IFN-β)基因轉染的人骨髓間充質榦細胞(hMSCs)對前列腺癌細胞株PC-3增殖及凋亡的影響.方法 脂質體介導入IFN-β基因轉染的hMSCs( IFN-β-h MSCs)與PC-3體外共培養,共培養後第1、3、5天對PC-3進行錐蟲藍染色,計數存活細胞數目.流式細胞儀分析PC-3細胞凋亡.結果 IFN-β-hMSCs可成功分泌錶達IFN-β.hMSCs、空質粒轉染hMSCs、IFN-β-hMSCs組與PC-3在共培養後第5天,PC-3存活細胞計數分彆為(10.81±0.33)×105、(9.90±0.41) ×105、(4.63±0.32)× 105箇,PC-3存活細胞差異有統計學意義(P＜0.01)；流式細胞儀檢測示實驗組PC-3凋亡率為(22.79±2.71)％,較各對照組增加.結論 IFN-β-hMSCs能成功分泌錶達IFN-β,體外共培養可有效抑製PC-3增殖,促進其凋亡.
목적 관찰인β간우소(IFN-β)기인전염적인골수간충질간세포(hMSCs)대전렬선암세포주PC-3증식급조망적영향.방법 지질체개도입IFN-β기인전염적hMSCs( IFN-β-h MSCs)여PC-3체외공배양,공배양후제1、3、5천대PC-3진행추충람염색,계수존활세포수목.류식세포의분석PC-3세포조망.결과 IFN-β-hMSCs가성공분비표체IFN-β.hMSCs、공질립전염hMSCs、IFN-β-hMSCs조여PC-3재공배양후제5천,PC-3존활세포계수분별위(10.81±0.33)×105、(9.90±0.41) ×105、(4.63±0.32)× 105개,PC-3존활세포차이유통계학의의(P＜0.01)；류식세포의검측시실험조PC-3조망솔위(22.79±2.71)％,교각대조조증가.결론 IFN-β-hMSCs능성공분비표체IFN-β,체외공배양가유효억제PC-3증식,촉진기조망.
Objective To investigate the effect of human interferon-β (IFN-β) gene cDNA engineered human bone marrow mesenchymal stem cells (hMSCs) on the proliferation and apoptosis of prostate cancer cell line PC-3.Methods The pCDNA3.1-IFN-β vector containing human IFN-β gene was constructed and transfected into hMSCs by lipofectamine.IFN-β-expressing mesenchymal stem cells (IFN-β-hMSCs) were incubated with PC-3 cells.PC-3 cells were stained by trypan blue and viable cells were counted by hemocytometer respectively at the 1 st,3rd,and 5 th day after co-culture.The apoptosis of PC-3 cells was analyzed by flow cytometry.Results IFN-β-hMSCs could express IFN-3 successfully.Five days after co-culture,the number of viable PC-3 cells in hMSCs,mock-hMSCs,IFN-β-hMSCs groups was ( 10.81 ± 0.33) × 105,(9.90 ± 0.41 ) × 105,and (4.63 ± 0.32 ) × 105 respectively.IFN-β-hMSCs resulted in a significant decrease in PC-3 cells survival as compared with control group (P ＜0.01 ),and apoptosis rate of PC-3 cells was (22.79 ±2.71 )％,which was increased as compared with control group.Conclusion The IFN-β-hMSCs can express IFN-β successfully and inhibit the growth of prostate cancer cells significantly.
