CAJ | 학술논문

目的观察脂质体携载前列腺素E1 (liposome prostaglandin E1,Lipo PGE1)对扩张皮瓣缺血-再灌注损伤的影响.方法采用中国小型猪背部扩张随意皮瓣缺血再灌注损伤模型,实验分为两组:实验组(Lipo PGE1干预组)及对照组.在其背部形成8 cm×2 cm扩张皮瓣,通过不同时间远端扩张皮瓣组织匀浆中髓过氧化酶(MPO)及丙二醛(MDA)含量测定,以及皮瓣微血管密度(MVD)、皮瓣存活率和皮温测定,观察Lipo PGE1对皮瓣成活的影响.结果实验组皮瓣存活率明显高于对照组(P＜0.05),术后两组MPO及MDA含量差异有统计学意义(P＜0.05),实验组明显低于对照组.两组MVD含量差异无统计学意义(P＞0.05).实验组用药后皮肤温度明显升高.结论应用Lipo PGE1可以减轻缺血-再灌注损伤,提高扩张皮瓣存活率.
목적 관찰지질체휴재전렬선소E1 (liposome prostaglandin E1,Lipo PGE1)대확장피판결혈-재관주손상적영향.방법 채용중국소형저배부확장수의피판결혈재관주손상모형,실험분위량조:실험조(Lipo PGE1간예조)급대조조.재기배부형성8 cm×2 cm확장피판,통과불동시간원단확장피판조직균장중수과양화매(MPO)급병이철(MDA)함량측정,이급피판미혈관밀도(MVD)、피판존활솔화피온측정,관찰Lipo PGE1대피판성활적영향.결과 실험조피판존활솔명현고우대조조(P＜0.05),술후량조MPO급MDA함량차이유통계학의의(P＜0.05),실험조명현저우대조조.량조MVD함량차이무통계학의의(P＞0.05).실험조용약후피부온도명현승고.결론 응용Lipo PGE1가이감경결혈-재관주손상,제고확장피판존활솔.
Objective To investigate the influence of the liposome PGE1 on the ischemia-reperfusion injury of the expanded flap.Methods The minipigs were set up as an animal model.They were divided into 2 groups.The expanded flaps (8 cm × 2 cm ) was designed and elevated on the back of the minipigs.An image analysis technology was used to evaluate the survival rate of the expanded flap,while a biochemistry method was used to detect the concentrations of malondialdehyde (MDA),myeloperoxidase (MPO) and microvessel density (MVD) in the tissue.Skin tempreture was measured after the drug administration.Results The survival area of the expanded flap in the Lipo PGE1 group was significantly enlarged (P＜0.05),compared with the control group.The MPO and MDA concen tration in the tissue was statistically different between two groups (P＜ 0.05),but there was no difference of the MVD concentration between two groups.The skin temperature was higher in the Lipo PGE1 group.Conclusions The Lipo PGE1 could promote survival rate in the expanded flap and protect it from ischemic injury.