CAJ | 학술논문

背景:人类β-防御素主要定位于多种组织的表皮或上皮.在眼表中也存在防御素,但其在眼表中的分布及眼表疾病中的作用尚不十分清楚.目的:观察人类β-防御素在眼表组织中的分布,分析其在眼表疾病中的潜在作用.设计、时间及地点:以眼表组织为观察对象体外对照实验,于2006-10/2007-12在上海交通大学医学院附属新华医院中心实验室和中国科学院上海分院细胞生化所完成.材料:18个炎性结膜样本中包括6个胬肉表面球结膜、4个球结膜囊肿、4个酸烧伤结膜组织、2个热烧伤结膜组织和2个结膜肉芽肿组织;15个炎性角膜样本中包括6个病毒性角膜炎、4个霉菌性角膜炎、3个细菌性角膜炎和2个角膜穿孔伤后摘除眼球;9个尸体眼正常球结膜样本,8个尸体眼正常角膜样本.方法:RT-PCR法及免疫组织化学法检测50份样本人类β-防御素表达.主要观察指标:人类β-防御素1～3三种蛋白因子在正常及炎症眼表组织的表达和定位.结果:RT-PCR检测发现,人类β-防御素1,3在所有被检角结膜组织中均显示阳性;人类β-防御素2在大部分炎症眼表组织中显示阳性,在正常眼表组织几乎不表达.免疫组织化学结果显示,大多数炎性眼表组织中均同时表达人类β-防御素1,2,分布于上皮细胞层,以基底层为主,偶见基质细胞的浸润,仅少数无人类β-防御素2表达;正常角膜和球结膜样本中均有人类β-防御素1表达,分布于上皮细胞层,以基底层为主,仅极少数同时存在人类β-防御素2表达.结论:人类β-防御素1,3呈组成式表达于正常及炎性眼表组织上皮细胞表皮与基底上皮细胞表层和基底层,人类β-防御素2则呈诱导式表达于大多数炎性眼表组织上皮细胞表皮与基底上皮细胞表层和基底层,均以基底层居多;3种人类β-防御素在对抗眼表感染和促进眼表损伤修复中可能起重要作用.
배경:인류β-방어소주요정위우다충조직적표피혹상피.재안표중야존재방어소,단기재안표중적분포급안표질병중적작용상불십분청초.목적:관찰인류β-방어소재안표조직중적분포,분석기재안표질병중적잠재작용.설계、시간급지점:이안표조직위관찰대상체외대조실험,우2006-10/2007-12재상해교통대학의학원부속신화의원중심실험실화중국과학원상해분원세포생화소완성.재료:18개염성결막양본중포괄6개노육표면구결막、4개구결막낭종、4개산소상결막조직、2개열소상결막조직화2개결막육아종조직;15개염성각막양본중포괄6개병독성각막염、4개매균성각막염、3개세균성각막염화2개각막천공상후적제안구;9개시체안정상구결막양본,8개시체안정상각막양본.방법:RT-PCR법급면역조직화학법검측50빈양본인류β-방어소표체.주요관찰지표:인류β-방어소1～3삼충단백인자재정상급염증안표조직적표체화정위.결과:RT-PCR검측발현,인류β-방어소1,3재소유피검각결막조직중균현시양성;인류β-방어소2재대부분염증안표조직중현시양성,재정상안표조직궤호불표체.면역조직화학결과현시,대다수염성안표조직중균동시표체인류β-방어소1,2,분포우상피세포층,이기저층위주,우견기질세포적침윤,부소수무인류β-방어소2표체;정상각막화구결막양본중균유인류β-방어소1표체,분포우상피세포층,이기저층위주,부겁소수동시존재인류β-방어소2표체.결론:인류β-방어소1,3정조성식표체우정상급염성안표조직상피세포표피여기저상피세포표층화기저층,인류β-방어소2칙정유도식표체우대다수염성안표조직상피세포표피여기저상피세포표층화기저층,균이기저층거다;3충인류β-방어소재대항안표감염화촉진안표손상수복중가능기중요작용.
BACKGROUND: Human β-defensin is mainly located in various tissues epidermis or epithelium, also exists in ocular surface, but its ocular surface and its role of ocular surface diseases remain poorly understood.OBJECTIVE: To observe the distribution of human β-defensins in ocular surface tissue, and to analyze their potential effects on ocular surface disease. DESIGN, TIME AND SETTING: In vitro controlled observation with regard to ocular surface tissue was performed at the Central Laboratory, Xinhua Hospital, Medical College of Shanghai Jiao Tong University and Cell Biochemistry Institute, Chinese Academy of Sciences Shanghai Branch, between October 2006 and December 2007.MATERIALS: A total of 18 inflammatory conjunctival specimens consisted of 6 pterygium surface bulbar conjunctiva, 4 bulbar conjunctival cysts, 4 acid burn conjunctiva, 2 thermal burn conjunctiva and 2 conjunctival granuloma; 15 inflammatory corneal specimens included 6 viral keratitis, 4 fungal keratitis, 3 bacterial keratitis and 2 eye removal following corneal perforation; 9 cadaver normal bulbar conjunctiva samples, 8 cadaver normal corneal samples. METHODS: RT-PCR method and immunohistochemistry were applied to detect human β-defensin expression in 50 samples. MAIN OUTCOME MEASURES: Distribution and location of human β-defensin proteins in normal and inflammatory ocular surface tissues. RESULTS: RT-PCR showed that human β-defensin 1 and human β-defensin 3 were positive in all of the tested samples, whereas human β-defensin 2 existed in a majority of inflammatory ocular surface tissues and no expression was observed in normal ocular surface tissues. Immunohistochemistry analysis revealed most of inflammatory ocular surface tissues expressed human β-defensins 1 and 2, distributing in epithelial cell layer and predominantly in basal lamina, occasionally infiltration of stromal cells was observed, only a small number of human β-defensin 2 expression was absent; normal cornea and conjunctiva samples presented with human β-defensin 1 expression, distributing in epithelial cells and predominantly in basal lamina, only few expressed human β-defensin 2.CONCLUSION: Human β-defensin 1 and 3 appear to be constitutively expressed in surface epithelial cells and basal lamina of normal and inflammatory ocular surface tissues, while human β-defensin 2 may be induced to express in the majority of inflammatory ocular surface tissues. Three human β-defensins expression plays a pivotal role in preventing ocular surface infection and promoting ocular surface injury repair.