CAJ | 학술논문

目的研究肉苁蓉多糖(CDPS)对小鼠淋巴细胞增殖的影响.方法 MTT法检测小鼠脾淋巴细胞的增殖.环磷酰胺(Cy)复制免疫功能低下的动物模型,分别测定正常及免疫低下动物脾脏、胸腺指数.胸腺细胞增殖法测定白细胞介素-2(IL-2)活性.结果 CDPS对丝裂原(ConA及LPS)活化淋巴细胞及未活化正常细胞均有明显促增殖作用,并促进淋巴细胞IL-2的分泌.腹腔给药显示CDPS具明显提高正常及免疫低下小鼠的脾指数,对因Cy所致胸腺指数的降低也有显著的对抗作用.结论 CDPS可显著促进小鼠脾淋巴细胞增殖,该作用可能与其促IL-2分泌有关.
목적 연구육종용다당(CDPS)대소서림파세포증식적영향.방법 MTT법검측소서비림파세포적증식.배린선알(Cy)복제면역공능저하적동물모형,분별측정정상급면역저하동물비장、흉선지수.흉선세포증식법측정백세포개소-2(IL-2)활성.결과 CDPS대사렬원(ConA급LPS)활화림파세포급미활화정상세포균유명현촉증식작용,병촉진림파세포IL-2적분비.복강급약현시CDPS구명현제고정상급면역저하소서적비지수,대인Cy소치흉선지수적강저야유현저적대항작용.결론 CDPS가현저촉진소서비림파세포증식,해작용가능여기촉IL-2분비유관.
Objective To study the effect of Cistanche deserticola Y C Ma (CDPS) on lymphocyte proliferation in mice. Methods The lymphocyte proliferation with or without mitogen was assessed by MTT assay in vitro. The immunosuppressed mice were induced by cyclophosphamide,and the spleen and thymus were weighted to determine the immune organ indexes in the normal or immunosuppressed mice. Thymocyte proliferation was employed to assess the activity of IL-2. Results CDPS significantly promoted both non-activated splenic lymphocytes and lymphocytes activated by ConA or LPS,and CDPS increased the secretion of IL-2 by splenic lymphocytes. CDPS (ip) remarkably increased indexes of spleen in normal or immunosuppressed mice,and also improved the indexes of immunosuppressed mice induced by cyclophosphamide. Conclusion CDPS can significantly promote the proliferation of splenic lymphocytes,and it may be related with promotion of secretion of IL-2 by splenocytes.