南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2010年
2期
232-235,238
,共5页
AY358935%生物信息学%分泌蛋白%双链RNA依赖蛋白激酶%水泡性口炎病毒
AY358935%生物信息學%分泌蛋白%雙鏈RNA依賴蛋白激酶%水泡性口炎病毒
AY358935%생물신식학%분비단백%쌍련RNA의뢰단백격매%수포성구염병독
AY358935%bioinformatics%secretary protein%double-stranded RNA-dependent protein kinase%vesicular stomatitis virus
目的 对新基因AY358935的功能性质进行生物信息分析,为深入研究该基因功能奠定基础.方法 利用生物信息学分析软件,分析新基因AY358935及其编码蛋白的性质、定位、结构、表达谱等特征,并根据相关信息预测其生物学功能.利用Western blotting检测病毒感染早期AY358935的表达变化.结果 AY358935基因进化保守,与马、小鼠、斑马鱼、非洲爪蟾的相似性分别为74%、60%、38%、33%.亚细胞定位于线粒体的可能性大.含有一个N末端信号肽序列和单次跨膜结构,多个磷酸化位点,二级结构主要是α螺旋和无规则卷曲.在正常组织和癌组织表达广泛,并受双链RNA依赖蛋白激酶的表达调控.AY358935蛋白表达在水泡性口炎病毒感染2h后即明显上调.结论初步预测AY358935为具有重要功能的小分子分泌蛋白,可能参与细胞增殖和抗病毒天然免疫调控.
目的 對新基因AY358935的功能性質進行生物信息分析,為深入研究該基因功能奠定基礎.方法 利用生物信息學分析軟件,分析新基因AY358935及其編碼蛋白的性質、定位、結構、錶達譜等特徵,併根據相關信息預測其生物學功能.利用Western blotting檢測病毒感染早期AY358935的錶達變化.結果 AY358935基因進化保守,與馬、小鼠、斑馬魚、非洲爪蟾的相似性分彆為74%、60%、38%、33%.亞細胞定位于線粒體的可能性大.含有一箇N末耑信號肽序列和單次跨膜結構,多箇燐痠化位點,二級結構主要是α螺鏇和無規則捲麯.在正常組織和癌組織錶達廣汎,併受雙鏈RNA依賴蛋白激酶的錶達調控.AY358935蛋白錶達在水泡性口炎病毒感染2h後即明顯上調.結論初步預測AY358935為具有重要功能的小分子分泌蛋白,可能參與細胞增殖和抗病毒天然免疫調控.
목적 대신기인AY358935적공능성질진행생물신식분석,위심입연구해기인공능전정기출.방법 이용생물신식학분석연건,분석신기인AY358935급기편마단백적성질、정위、결구、표체보등특정,병근거상관신식예측기생물학공능.이용Western blotting검측병독감염조기AY358935적표체변화.결과 AY358935기인진화보수,여마、소서、반마어、비주조섬적상사성분별위74%、60%、38%、33%.아세포정위우선립체적가능성대.함유일개N말단신호태서렬화단차과막결구,다개린산화위점,이급결구주요시α라선화무규칙권곡.재정상조직화암조직표체엄범,병수쌍련RNA의뢰단백격매적표체조공.AY358935단백표체재수포성구염병독감염2h후즉명현상조.결론초보예측AY358935위구유중요공능적소분자분비단백,가능삼여세포증식화항병독천연면역조공.
Objective To obtain the functional information of AY358935 gene. Methods The properties, subeellular location,and structure of AY358935 protein, and the expression profile of AY358935 gene were analyzed by bioinformatics software and the biological functions of the gene were predicted. AY358935 expression was detected by Western blot analysis in early virus infection. Results AY358935 was evolutionally conserved. The human AY358935 protein had an amino acid similarity of 74%, 60%, 38% and 33% with its counterpart in horses, mice, zebrafish and Xenopus laevis, respectively. Bioinformatics analysis indicated that AY358935 protein was located likely in the mitochondria. There was a N-terminal signal peptide and single transmembrane structure in AY358935 protein, which contained several phosphorylation sites. The secondary structure mainly comprised of alpha helices and random coils. AY358935 was ubiquitously expressed in normal tissues and carcinomas and regulated by the expression of double-stranded RNA-dependent protein kinase. AY358935 protein expression was obviously upregulated in cells 2 h after infection by vesicular stomatitis virus. Conclusion As a predicted secretary protein with a small molecular weight, AY358935 might have important functions in cellular proliferation and anti-viral innate immune regulation.
