CAJ | 학술논문

目的研究硒化壳聚糖对体外培养的NB4细胞增殖和凋亡的影响,探讨这种作用与端粒酶活性和survivin蛋白的关系.方法 MTT法检测细胞增殖;细胞形态学观察和流式细胞仪检测细胞凋亡;PCR-ELISA法检测端粒酶活性;western blot法检测survivin蛋白含量.结果 50～200 mg/L硒化壳聚糖可时间剂量依赖性地抑制NB4细胞增殖,诱导其凋亡,降低端粒酶活性和下调survivin蛋白含量.结论硒化壳聚糖可通过抑制端粒酶活性,下调survivin蛋白含量,诱导细胞凋亡从而抑制体外培养的NB4细胞增殖.
목적 연구서화각취당대체외배양적NB4세포증식화조망적영향,탐토저충작용여단립매활성화survivin단백적관계.방법 MTT법검측세포증식;세포형태학관찰화류식세포의검측세포조망;PCR-ELISA법검측단립매활성;western blot법검측survivin단백함량.결과 50～200 mg/L서화각취당가시간제량의뢰성지억제NB4세포증식,유도기조망,강저단립매활성화하조survivin단백함량.결론 서화각취당가통과억제단립매활성,하조survivin단백함량,유도세포조망종이억제체외배양적NB4세포증식.
Objective To investigate the effect of selenium chitosan on proliferation and apoptosis in NB4 cells and explore its relationship with the telomerase activity and survivin protein. Methods MTT was used to assess the cell proliferation; Microscopic observation and flow cytometry were used to assess the induced apoptosis; Polymerase chain reaction enzyme-linked immunoassay (PCR-ELISA) was used to measure the telomerase activity; Western blot was used to examine the expression level of survivin protein. Results Exposure to 50～200 mg/L of selenium chitosan for 24～72 h inhibited proliferation and induced apoptosis in NB4 cells in dose and time dependent manners. The telomerase activity and expression of survivin protein were strongly inhibited in selenium chitosan treated NB4 cells. Conclusion Selenium chitosan inhibites NB4 cells proliferation in vitro via reducing telomerase activity and down-regulating survin protein and induces apoptosis.