CAJ | 학술논문

为了检测抗生素提出了采用生物分子相互作用原理的检测方法.采用基于表面等离子体共振技术的光学传感器,通过自组装分子技术将单克隆抗体固定到传感器金膜表面用于检测对应抗原,即待测抗生素,并记录传感器表面液体折射率的变化曲线,其反映了被吸附抗生素的浓度的大小.对不同浓度的氨苄青霉素水溶液进行了测定,得到该传感器的工作曲线,整体工作曲线呈单调上升,且线性关系较好,同时得到了该检测方法的最低检测极限为1.25 ng/mL,明显低于欧盟规定的最大残留限量(4 μg/kg).进一步分析了流速、温度、pH值和离子浓度对检测的影响,获得了该检测方法的最佳测量条件,提高了该方法的检测精度,从而保障了该方法的有效性.
위료검측항생소제출료채용생물분자상호작용원리적검측방법.채용기우표면등리자체공진기술적광학전감기,통과자조장분자기술장단극륭항체고정도전감기금막표면용우검측대응항원,즉대측항생소,병기록전감기표면액체절사솔적변화곡선,기반영료피흡부항생소적농도적대소.대불동농도적안변청매소수용액진행료측정,득도해전감기적공작곡선,정체공작곡선정단조상승,차선성관계교호,동시득도료해검측방법적최저검측겁한위1.25 ng/mL,명현저우구맹규정적최대잔류한량(4 μg/kg).진일보분석료류속、온도、pH치화리자농도대검측적영향,획득료해검측방법적최가측량조건,제고료해방법적검측정도,종이보장료해방법적유효성.
A method based on bio-molecular interaction analysis(BIA)was proposed for the detection of antibiotics residues in milk by adopting the surface plasmon resonance(SPR)biosensor.Monoclonal antibodies were immobilized on the gold surface of SPR biosensor to detect corresponding antigens-antibiotics.Refractive index (RI)curves of different liquids,which are recorded by SPR biosensor,are related to concentrations of antibiotics in milk.Water solution with a series of ampicillin concentrations was detected and analyzed in this paper.The whole working curve with good linearity of the sensor was acquired.The lowest limit using this method reached 1.25 ng/mL,which was much lower than maximum residue limit(MRL:4 μg/kg)set by European Union.Furthermore,the influencing factors such as flow velocity of liquid,temperature,pH range and ion concentrations were discussed in order to get the optimum experiment conditions for the method and ensure its effectiveness and high accuracy.