CAJ | 학술논문

目的:观察姜黄素-聚维酮固体分散体新剂型(Cur-K30)的体内外抗肿瘤作用及其对肿瘤血管形成的影响.方法:应用MTT法测定Cur-K30体外对多种肿瘤细胞的抑制作用;观察Cur-K30不同剂量[50,100,200 mg/(kg·d)]口服给药对小鼠荷瘤模型的抑瘤作用;应用免疫组织化学方法检测瘤组织的微血管密度(CD34)和血管内皮生长因子(VEGF)的表达,并应用Imageproplus免疫组织化学软件定量分析.结果:Cur-K30抑制多种肿瘤细胞的增殖,其48 h的IC50为6.6～12.12 μg/mL;200 mg/(kg·d) Cur-K30灌胃对小鼠肝癌H22移植瘤、小鼠黑色素瘤B16移植瘤和裸鼠SW480的抑制率分别为43.2%,53.1%, 59.8%,抑瘤率高于同剂量姜黄素(Cur)混悬液灌胃组,差别均具有统计学意义(P<0.01);200 mg/(kg·d) Cur-K30组的瘤组织中CD34和VEGF的表达较对照组明显下调(P<0.01).结论:Cur-K30具有明显的体内抗肿瘤作用,其对裸鼠人癌细胞SW480移植瘤的抑制作用显著.Cur-K30的抗肿瘤作用可能与其下调瘤组织中VEGF的表达及抑制肿瘤血管生成有关.
목적:관찰강황소-취유동고체분산체신제형(Cur-K30)적체내외항종류작용급기대종류혈관형성적영향.방법:응용MTT법측정Cur-K30체외대다충종류세포적억제작용;관찰Cur-K30불동제량[50,100,200 mg/(kg·d)]구복급약대소서하류모형적억류작용;응용면역조직화학방법검측류조직적미혈관밀도(CD34)화혈관내피생장인자(VEGF)적표체,병응용Imageproplus면역조직화학연건정량분석.결과:Cur-K30억제다충종류세포적증식,기48 h적IC50위6.6～12.12 μg/mL;200 mg/(kg·d) Cur-K30관위대소서간암H22이식류、소서흑색소류B16이식류화라서SW480적억제솔분별위43.2%,53.1%, 59.8%,억류솔고우동제량강황소(Cur)혼현액관위조,차별균구유통계학의의(P<0.01);200 mg/(kg·d) Cur-K30조적류조직중CD34화VEGF적표체교대조조명현하조(P<0.01).결론:Cur-K30구유명현적체내항종류작용,기대라서인암세포SW480이식류적억제작용현저.Cur-K30적항종류작용가능여기하조류조직중VEGF적표체급억제종류혈관생성유관.
Objective To evaluate the anti-proliferation and anti-angiogenesis effect of curcumin-K30 solid dispersion (Cur-K30) on tumors in vivo. Methods Growth inhibition rates of the tumor cells was measured with MTT method. Tumor inhibition was detected by tumors transplanted subcutaneously in mice treated with Cur-K30 ［50， 100， and 200 mg/(kg·d)］. The expressions of CD34 and vascular endothelial growth factor (VEGF) were assessed by immunohistochemical study, and analyzed by Imageproplus software. Results Cur-K30 had inhibitory effect on different tumor cell lines in a dose dependent manner with IC50 values from 6.6 to 12.12 μg/mL. The in vivo study showed that the inhibitory rates of the 200 mg/(kg·d) Cur-K30 group on H22, B16, and SW480 were 43.2%, 53.1%, and 59.8%, respectively, which were all much higher than the inhibitory rates of curcumin suspension group with the same dose. Compared with the control group， the expression of CD34 and VEGF in SW480 tumors was down-regulated in the 200 mg/(kg·d) Cur-K30 group (P<0.01). Conclusion The proliferation inhibition of Cur-K30 is higher than curcumin in vivo， and the most significant effect is obtained in SW480 tumors transplanted subcutaneously in nude mice. Down-regulation of VEGF and decreased microvascular density may contribute to the anti-tumor effect of Cur-K30.