CAJ | 학술논문

通过序列比对分析鉴定出9个GSK同源基因(命名为OsGSK1 -9),它们分布在水稻的6条染色体上.聚类分析表明预测的OsGSK蛋白和其他植物中的GSK蛋白可被分为4个亚组.通过实时定量PCR进一步分析了OsGSK基因家族的基因在水稻各种组织和器官以及在多种逆境胁迫和植物激素处理条件下的表达量.结果表明:大多数OsGSK基因在水稻全生育期都有较高的表达量并且受多种激素(如脱落酸、生长素、油菜素内酯)和逆境(如干旱和盐胁迫)胁迫诱导表达,表明OsGSK基因家族在水稻发育和逆境适应过程中可能起重要作用.
통과서렬비대분석감정출9개GSK동원기인(명명위OsGSK1 -9),타문분포재수도적6조염색체상.취류분석표명예측적OsGSK단백화기타식물중적GSK단백가피분위4개아조.통과실시정량PCR진일보분석료OsGSK기인가족적기인재수도각충조직화기관이급재다충역경협박화식물격소처리조건하적표체량.결과표명:대다수OsGSK기인재수도전생육기도유교고적표체량병차수다충격소(여탈락산、생장소、유채소내지)화역경(여간한화염협박)협박유도표체,표명OsGSK기인가족재수도발육화역경괄응과정중가능기중요작용.
The glycogen synthase kinase 3 (GSK3)/SHAGGY-like kinases are serine/threonine protein kinases involved in a variety of biological processes.In this study,nine GSK3-like genes (OsGSK1-9) were identified in rice,and they are distributed on six chromosomes,and the distribution pattern is related to the chromosomal block duplication events in rice.The OsGSK proteins can be classified into four subgroups.The expression patterns of OsGSK genes were investigated in various tissues and organs of rice and in the seedlings treated with phytohormones and abiotic stresses.The results suggest that most of OsGSK genes have high expression level in the whole life cycle,and they are responsive not only to different phytohormones (such as abscisic acid,auxin,and brassinosteroid) but also to drought and salt stresses,implying that OsGSK genes may have important roles in development and stress responses in rice.