CAJ | 학술논문

目的观察大鼠视神经夹伤后对闪光视觉诱发电位(F-VEP)的影响及睫状神经营养因子(ciliary neurotrophic factor,CNTF)、睫状神经营养因子受体(ciliary neurotrophic factor recepter,CNTFR)、少突胶质细胞髓鞘糖蛋白(oligodendrocyte myelin glycoprotein,OMgp)在视网膜中的表达变化.方法采用夹持视神经方法建立大鼠视神经不完全损伤模型,在夹伤后1、3、7、14和28d剥离视网膜,提取总RNA用半定量逆转录聚合酶反应(rt-PCR)方法测定视网膜中CNTF,CNTFR,OMgp mRNA的表达,同时观测术后1、7、14和28dF-VEP波形改变.结果大鼠视神经损伤后视网膜中CNTF,CNTFR,OMgp mRNA有所增加,正常大鼠视网膜中CNTF,CNTFR,OMgp少量表达.视神经损伤后F-VEP潜伏期延长,波幅降低,波形低而宽,14d后有所恢复.结论大鼠视神经损伤后,CNTF,CNTFR,OMgp表达均增加,而CNTFR的增加可为外源性CNTF治疗视神经损伤提供依据.F-VEP的振幅,潜时伤后变化与时间相关,伤后14d变化最明显,以后有恢复迹象.
목적 관찰대서시신경협상후대섬광시각유발전위(F-VEP)적영향급첩상신경영양인자(ciliary neurotrophic factor,CNTF)、첩상신경영양인자수체(ciliary neurotrophic factor recepter,CNTFR)、소돌효질세포수초당단백(oligodendrocyte myelin glycoprotein,OMgp)재시망막중적표체변화.방법 채용협지시신경방법건립대서시신경불완전손상모형,재협상후1、3、7、14화28d박리시망막,제취총RNA용반정량역전록취합매반응(rt-PCR)방법측정시망막중CNTF,CNTFR,OMgp mRNA적표체,동시관측술후1、7、14화28dF-VEP파형개변.결과 대서시신경손상후시망막중CNTF,CNTFR,OMgp mRNA유소증가,정상대서시망막중CNTF,CNTFR,OMgp소량표체.시신경손상후F-VEP잠복기연장,파폭강저,파형저이관,14d후유소회복.결론 대서시신경손상후,CNTF,CNTFR,OMgp표체균증가,이CNTFR적증가가위외원성CNTF치료시신경손상제공의거.F-VEP적진폭,잠시상후변화여시간상관,상후14d변화최명현,이후유회복적상.
Objective To investigate the effect of the optic injury to F-VEP and the change of CNTF CNTFR and OMgpmRNA.Mthod establish model of inadequate injury of rats'optic nerve and use rt-PCR technique to measure the expression of CNTF CNTFR and OMgpmRNA at the time of 1, 3,7,14,28 day after the injury.We also analyse the change of rats'F-VEP.Result the 7d's expressions of CNTF, CNTFR,OMgp mRNA were more than the 2w'expessions.And mRNA of CNTF, CNTFR, OMgp were expressed in normal PEGs.Compared with that of the normal rats, the latency of F-VEP in optic nerve crushed rats increased and the wave of F-VEP became lower and wider.And recovery response was observed after 14 days.Conclusions After the optic nerve injury, levels of CNTF CNTFR and OMgpmRNA increased. CNTF CNTFR mRNA are likely to protect the degeneration of nerve cells by optic nerve injury, So exogenous CNTF might provide a supportive emviroment for axonal regeneration. F-VEP correlate with the time of postinjury.14days changed prominently and recovery start after 14 days.