CAJ | 학술논문

目的检测胃下部癌患者常规病理阴性第11P组淋巴结微转移的情况,分析淋巴结微转移与临床病理因素的关系.方法应用连续切片法和端粒酶重复扩增-ELISA方法检测43例胃下部癌常规病理阴性的43枚第11P组淋巴结,结合临床病理资料进行统计学分析. 结果本组43例胃下部癌患者常规病理阴性第11P组淋巴结经连续切片法检出有4例4枚淋巴结发生微转移,微转移发生率为9%;应用端粒酶重复扩增-ELISA法检测微转移发生率为44%,其中包括应用连续切片法检测出有微转移的4枚淋巴结.端粒酶重复扩增-ELISA法微转移检出率明显高于连续切片法(x 2 =13.07,P<0.05).胃下部癌第11P组淋巴结微转移与原发肿瘤大小(x 2 =8.488,P<0.05)、浸润深度(x 2 =6.473,P<0.05)及临床分期(x 2 =12.022,P<0.05)有关,与患者年龄、性别、大体分型、组织分化程度尤关.结论胃下部癌常规病理阴性第11P组淋巴结中存在较高的微转移发生率,其微转移发生率与原发肿瘤大小、浸润深度及临床分期有关.
목적 검측위하부암환자상규병리음성제11P조림파결미전이적정황,분석림파결미전이여림상병리인소적관계.방법 응용련속절편법화단립매중복확증-ELISA방법 검측43례위하부암상규병리음성적43매제11P조림파결,결합림상병리자료진행통계학분석. 결과 본조43례위하부암환자상규병리음성제11P조림파결경련속절편법검출유4례4매림파결발생미전이,미전이발생솔위9%;응용단립매중복확증-ELISA법검측미전이발생솔위44%,기중포괄응용련속절편법검측출유미전이적4매림파결.단립매중복확증-ELISA법미전이검출솔명현고우련속절편법(x 2 =13.07,P<0.05).위하부암제11P조림파결미전이여원발종류대소(x 2 =8.488,P<0.05)、침윤심도(x 2 =6.473,P<0.05)급림상분기(x 2 =12.022,P<0.05)유관,여환자년령、성별、대체분형、조직분화정도우관.결론 위하부암상규병리음성제11P조림파결중존재교고적미전이발생솔,기미전이발생솔여원발종류대소、침윤심도급림상분기유관.
Objective To evaluate the status of lymph node micrometastases in "non-metastatic" No11P lymph nodes as judged by conventional pathology in the lower third of gastric cancer. Methods In this study 43 No11P lymph nodes harvested from 43 patients which was histologically free of metastasis were examined by consecutive sections and TRAP( telomeric repeat amplification protocol)-ELISA (enzyme linked immunosorbent assay). The data were statistically analyzed according to the clinicopathological features of the patients. Results Micrometastasis was discovered in 4 lymph nodes from 4 patients by consecutive sections. The micrometastatic rate of the conventional pathologic non-metastatic No11P lymph nodes was 9%. The micrometastatic rate of the conventional pathologic non-metastasis No11P lymph nodes detected by TRAP-ELISA was 44%, including 4 lymph nodes observed by consecutive sections It revealed that lymph nodes micrometastases were correlated with the size of the tumor( x2 = 8. 488, P < 0. 05 )、and tumor stage (x2 = 12. 022,P < 0. 05 ). It also showed that the micrometastatic rate increased proportionally to tumor infiltration depth(x2 =6. 473, P <0. 05), not correlated with patients' demographic features, general type and histological differentiation of the tumor. Conclusions There was a high rate of micrometastasis in No11P lymph nodes. This lymph nodes micrometastasis was correlated with the size of the tumor, invasion depth of primary tumor and patients' clinical stage.