CAJ | 학술논문

目的建立种植体植入动物模型,研究低强度脉冲超声(low intensity pulsed ultrasound,LIPUS)对种植体骨结合的促进作用.方法选用雌性SD大鼠24只,每只大鼠双侧胫骨干骺端内侧植入螺纹状钛种植体.植入术后第2天对右侧胫骨种植体周围进行LIPUS刺激作为实验侧,每日20 min,左侧胫骨种植体植入部位不刺激作为自身对照侧.超声刺激后第4、8、12 周分批处死动物,每批8只,获取双侧胫骨标本.采用X线片、显微CT、组织切片等观察LIPUS对种植体骨结合的作用.结果 3个时间点种植体与骨组织之间均形成了不同程度的骨结合.显微CT组织形态计量学分析结果显示,种植体周骨结合率在实验侧第4、8、12周分别为(45.708±3.316)%、(46.231±1.954)%、(46.807±1.451)%,在对照侧分别为(43.021±3.558)%、(44.272±3.023)%、(44.894±4.215)%,第4周时差异具有统计学意义(P<0.05).种植体周骨体积分数在第4、8周,种植体周骨小梁厚度及第8周、第12周各向异性程度,实验侧均高于自身对照侧,差异有统计学意义(P<0.05).组织学切片可见实验侧种植体周新生骨组织厚度明显大于自身对照侧,骨结合量更多.结论 LIPUS可以促进种植体周围骨组织的愈合,加快种植体骨结合,并能促进骨小梁早期呈一定方向改建.
목적 건립충식체식입동물모형,연구저강도맥충초성(low intensity pulsed ultrasound,LIPUS)대충식체골결합적촉진작용.방법 선용자성SD대서24지,매지대서쌍측경골간후단내측식입라문상태충식체.식입술후제2천대우측경골충식체주위진행LIPUS자격작위실험측,매일20 min,좌측경골충식체식입부위불자격작위자신대조측.초성자격후제4、8、12 주분비처사동물,매비8지,획취쌍측경골표본.채용X선편、현미CT、조직절편등관찰LIPUS대충식체골결합적작용.결과 3개시간점충식체여골조직지간균형성료불동정도적골결합.현미CT조직형태계량학분석결과현시,충식체주골결합솔재실험측제4、8、12주분별위(45.708±3.316)%、(46.231±1.954)%、(46.807±1.451)%,재대조측분별위(43.021±3.558)%、(44.272±3.023)%、(44.894±4.215)%,제4주시차이구유통계학의의(P<0.05).충식체주골체적분수재제4、8주,충식체주골소량후도급제8주、제12주각향이성정도,실험측균고우자신대조측,차이유통계학의의(P<0.05).조직학절편가견실험측충식체주신생골조직후도명현대우자신대조측,골결합량경다.결론 LIPUS가이촉진충식체주위골조직적유합,가쾌충식체골결합,병능촉진골소량조기정일정방향개건.
Objective To establish an animal model of titanium implant and to investigate the effects of low intensity pulsed ultrasound(LIPUS)on the implant osseointegration. Methods Twenty-four female Sprague Dawley(SD)rats were used. Titanium implants were inserted into tibia metaphysis bilaterally in each SD rat. Since the second day following implant surgery, LIPUS stimulation were carried out around the implant on the right side for 20 minutes per day, and implants on the left side remain untouched as self-control. Eight rats were sacrificed and tibiae specimens were harvested on the end of the 4th, 8th, and 12th week respectively. X-ray picture,micro-CT analysis and histological sections were performed to evaluate the effects of LIPUS on the implant osseointegration. Results Osseointegration at the interface between the implant and bone tissue was observed bilaterally. In the micro-CT histomorphometry analysis, the percentage of osseointegration(%OI)of experiment side were (45.708±3.316)%, (46.231±1.954)%, and (46.807±1.451)% on the end of the 4th, 8th, and 12th week. Meanwhile, the %OI of control side were (43.021±3.558)%, (44.272±3.023)%, and (44.894±4.215)% respectively. The %OI on the 4th weekend, bone volume ratio(BV/TV)on the 4th and 8th weekend,trabecular thickness(Tb. Th)and degree of anisotropy(DA)on the 8th and 12th weekend of the experiment side tibia were significantly greater than those on the control side(P<0.05). The histological examination showed that the thickness of new bone around the implant and the bone-implant contact area was obviously greater in the LIPUS treated side compared to the control side.Conclusions The LIPUS therapy may accelerate the bone healing and osseointegration at the interface between titanium implant and bone, and promote remodeling of bone trabecula on the early stage.