CAJ | 학술논문

目的观察猪自体骨髓单个核细胞移植后心肌梗死区干细胞因子、基质细胞衍生因子-1、E-选择素、细胞间粘附分子-1、血管细胞粘附因子-1等细胞因子mRNA的表达.方法前降支球囊封堵法建立10头猪急性心肌梗死动物模型,按随机数字表法分为对照组(5头)和移植组(5头).造模1周后,移植组冠脉内移植自体骨髓单个核细胞,对照组注射1640培养基作为对照.6个月后取梗死区心脏组织观察局部细胞因子的mRNA表达.结果移植组梗死区能检测到基质细胞衍生因子-1、干细胞因子、E-选择素、细胞间粘附分子-1、血管细胞粘附分子-1等细胞因子的mRNA.其表达强度明显高于对照组.结论干细胞移植能促进局部的基质细胞衍生因子-1、干细胞因子、E-选择素、细胞间粘附分子-1和血管细胞粘附分子-1 mRNA的表达.
목적 관찰저자체골수단개핵세포이식후심기경사구간세포인자、기질세포연생인자-1、E-선택소、세포간점부분자-1、혈관세포점부인자-1등세포인자mRNA적표체.방법 전강지구낭봉도법건립10두저급성심기경사동물모형,안수궤수자표법분위대조조(5두)화이식조(5두).조모1주후,이식조관맥내이식자체골수단개핵세포,대조조주사1640배양기작위대조.6개월후취경사구심장조직관찰국부세포인자적mRNA표체.결과 이식조경사구능검측도기질세포연생인자-1、간세포인자、E-선택소、세포간점부분자-1、혈관세포점부분자-1등세포인자적mRNA.기표체강도명현고우대조조.결론 간세포이식능촉진국부적기질세포연생인자-1、간세포인자、E-선택소、세포간점부분자-1화혈관세포점부분자-1 mRNA적표체.
Objective To investigate the effects of bone marrow stem cells autologous transplantation after acute myocardial infarction on local mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1and VCAM-1.Methods Acute myocardial infarction models were established in 10 swine,which were randomized divided into placebo group (5 swine) and transplantation group (5 swine).After six months,hearts of placebo group and early transplantation group were harvested and mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1 and VCAM-1 in infarcted myocardial region was detected.Results The mRNAs of SDF-1,Kit ligand,E-selection,ICAM-1 and VCAM-1 were expressed in the infarcted myocardial region of transplantation group six months after myocardial infarction,but it was not found in the placebo group.Conclusions Bone marrow autologous transplantation after acute myocardial infarction can promote local mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1 and VCAM-1,which may be due to the paracrine effects of stem cells.