CAJ | 학술논문

目的初步探讨不同浓度阿奇霉素作用下沙眼衣原体的超微结构形态变化.方法采用McCoy细胞培养方法,在沙眼衣原体实验菌株(D/UW-3/Cx)体外培养过程中添加含阿奇霉素质量浓度为0.0667、0.1340、0.1900、0.2680、0.3330 mg/L的培养液,不加阿奇霉素的培养液作为空白对照组.在透射电镜下观察McCoy细胞和沙眼衣原体超微结构的变化.结果在接种D/UW-3/Cx时加入阿奇霉素培养48 h后,随阿奇霉素浓度的增加,透射电镜下可见包涵体内外囊泡增多;有异常增大的网状体,部分存在分裂异常,甚至网状体坏死崩解;原体数目减少,体积增大,外膜皱褶增多.阿奇霉素质量浓度为0.3330 mg/L时见不到包涵体.结论阿奇霉素可引起沙眼衣原体外膜膨大,形成囊泡;网状体异常增大,甚至崩解;原体减少.
목적 초보탐토불동농도아기매소작용하사안의원체적초미결구형태변화.방법 채용McCoy세포배양방법,재사안의원체실험균주(D/UW-3/Cx)체외배양과정중첨가함아기매소질량농도위0.0667、0.1340、0.1900、0.2680、0.3330 mg/L적배양액,불가아기매소적배양액작위공백대조조.재투사전경하관찰McCoy세포화사안의원체초미결구적변화.결과 재접충D/UW-3/Cx시가입아기매소배양48 h후,수아기매소농도적증가,투사전경하가견포함체내외낭포증다;유이상증대적망상체,부분존재분렬이상,심지망상체배사붕해;원체수목감소,체적증대,외막추습증다.아기매소질량농도위0.3330 mg/L시견불도포함체.결론 아기매소가인기사안의원체외막팽대,형성낭포;망상체이상증대,심지붕해;원체감소.
Objective To observe the ultrastructural changes of Chlamydia trachomatis after treatment with azithromycin.Methods The Chlamydia trachomatis laboratory strain (D/UW-3/Cx) was cultured in McCoy cells with or without the presence of azithromycin of 0.0667,0.1340,0.1900,0.2680 and 0.3330 mg/L for 48 hours.The ultrastructural changes of host cells and Chlamydia trachomatis were observed by transmission electron microscopy.Results After 48-hour culture,vesicles increased in number both inside and outside of the inclusion bodies with the rise in azithromycin concentration; there were abnormally large reticulate bodies,some of which experienced abnormal division and even necrosis or breakdown; the number of elementary bodies was decreased,while their size was enlarged,with a more wrinkled outer membrane.No inclusion bodies were seen when the concentration of azithromycin was 0.333 mg/L. Conclusions Azithromycin can induce an increment in the outer membrane of Chlamydia trachomatis,formation of vesicles,abnormal enlargement or breakdown of reticulate bodies,and a decrease in elementary bodies.