CAJ | 학술논문

将150例行髓芯减压加自体干细胞移植股骨头坏死患者按侧别分为单侧和双侧髂骨取血两组,按取血量冉分为3个亚组(200、300和400 ml组),并于取髂骨血前、后行单核细胞记数.单侧髂骨取血200、300和400 ml组分离后单核细胞记数分别为(3.11 4-1.42)×1010/L、(2.62±1.31)x 1010/L和(2.13±0.91)x 1010/L,取血400 ml组单核细胞记数显著低于取血200 ml组(t=5.118,P<0.01);双侧髂骨取血200、300和400 ml组分离后单核细胞计数分别为(4.51±2.21)x1010/L、(3.89±2.06)x 1010/L和(2.98±1.39)x 1010/L,取血400 ml组单核细胞记数显著低于取血200 ml组(t=6.446,P<0.01);双侧髂骨取血200、300和400 ml分离后单核细胞浓度明显高于单侧取血,且差异有统计学意义(t=3.119,P<0.01;t=5.544,P<0.01;t=2.207,P<0.05).提示髂骨取血所分离单核细胞浓度受不同取血方式影响:双侧高于单侧;随着取血剂量增加,分离单核细胞浓度降低.
장150례행수심감압가자체간세포이식고골두배사환자안측별분위단측화쌍측가골취혈량조,안취혈량염분위3개아조(200、300화400 ml조),병우취가골혈전、후행단핵세포기수.단측가골취혈200、300화400 ml조분리후단핵세포기수분별위(3.11 4-1.42)×1010/L、(2.62±1.31)x 1010/L화(2.13±0.91)x 1010/L,취혈400 ml조단핵세포기수현저저우취혈200 ml조(t=5.118,P<0.01);쌍측가골취혈200、300화400 ml조분리후단핵세포계수분별위(4.51±2.21)x1010/L、(3.89±2.06)x 1010/L화(2.98±1.39)x 1010/L,취혈400 ml조단핵세포기수현저저우취혈200 ml조(t=6.446,P<0.01);쌍측가골취혈200、300화400 ml분리후단핵세포농도명현고우단측취혈,차차이유통계학의의(t=3.119,P<0.01;t=5.544,P<0.01;t=2.207,P<0.05).제시가골취혈소분리단핵세포농도수불동취혈방식영향:쌍측고우단측;수착취혈제량증가,분리단핵세포농도강저.
One hundred and fifty femoral head necrosis patients with intramedullary decompression received autologous stem cell transplantation. The blood was taken from unilateral or bilateral iliac, in each group the volumes of blood taken were 200, 300 or 400 ml respectively. The number of mononuclear cells was counted before and after centrifugation. The mononuclear cell count in subgroup of 200 ml,300 ml and 400 ml taken from unilateral iliac after centrifugation was (3. 11 ±1. 42) × 1010/L, (2. 62 ±1. 31 ) × 1010/L and(2. 13 ±. 91) × 1010/L. The concentration was significantly lower in subgroup of 400 ml than that of 200 ml (t=5. 118, P<0. 01). The mononuclear cells count in subgroup of 200 ml,300 ml and 400 ml taken from bilateral iliac was(4. 51 ±. 21) × 1010/L, (3. 89 ±. 06) × 1010/L and (2. 98 ±1. 39) × 1010/L, the concentration was significantly lower in subgroup of 400 ml than that of subgroup of 200 ml (t = 6. 446, P < 0. 01). Whether 200 ml or 300 ml or 400 ml blood were taken, the mononuclear cell count in blood taken from bilateral iliac after centrifugation was significantly higher than that from unilateral iliac(t = 3. 119,P < 0. 01; t = 5. 544, P < 0. 01 ;t = 2. 207 ,P < 0. 05). The results indicate that the concentration of isolated bone marrow mononuclear cells is higher from bilateral iliac than unilateral iliac. The concentration of isolated monocytes is reduced gradually with the increased blood volume.