中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
36期
2578-2581
,共4页
结肠肿瘤%骨桥蛋白%细胞间隙连接通信
結腸腫瘤%骨橋蛋白%細胞間隙連接通信
결장종류%골교단백%세포간극련접통신
Colorectal neoplasms%Osteopontin%Gap junctional intercellular communication
目的 检测骨桥蛋白( Osteopontin，OPN)转染后大肠癌SW-480细胞株的细胞间隙连接通信( gap junctional intercellular communication，GJIC)改变。方法 以OPN真核表达质粒转染大肠癌SW-480细胞株后，利用激光共聚焦显微镜（laser scanning confocal microscope，LSCM），运用荧光淬灭后恢复技术（flurescence redistribution after photobleaching，FRAP）检测OPN转染后SW-480细胞的GJIC功能变化。结果 转染OPN后，高表达OPN的SW-480-pcDNA3.1(+)-OPN细胞株淬灭5 min 后荧光恢复率为24.65％±4.08％，而对照组SW-480-pcDNA3.1(+)为44.74％ ±6.23％，P＜0.001；10 min后荧光恢复率SW-480-pcDNA3.1(+)-OPN仅为25.98％±4.48％，SW-480-pcDNA3.1(+)已上升至64.92％±5.39％，P＜0.001。转染后高表达OPN的SW-480细胞，GJIC功能被抑制，细胞之间通信减弱，淬灭后恢复率相比较对照组明显降低。结论 OPN减弱大肠癌细胞间的通信能力，可能与大肠癌细胞的侵袭转移相关。
目的 檢測骨橋蛋白( Osteopontin，OPN)轉染後大腸癌SW-480細胞株的細胞間隙連接通信( gap junctional intercellular communication，GJIC)改變。方法 以OPN真覈錶達質粒轉染大腸癌SW-480細胞株後，利用激光共聚焦顯微鏡（laser scanning confocal microscope，LSCM），運用熒光淬滅後恢複技術（flurescence redistribution after photobleaching，FRAP）檢測OPN轉染後SW-480細胞的GJIC功能變化。結果 轉染OPN後，高錶達OPN的SW-480-pcDNA3.1(+)-OPN細胞株淬滅5 min 後熒光恢複率為24.65％±4.08％，而對照組SW-480-pcDNA3.1(+)為44.74％ ±6.23％，P＜0.001；10 min後熒光恢複率SW-480-pcDNA3.1(+)-OPN僅為25.98％±4.48％，SW-480-pcDNA3.1(+)已上升至64.92％±5.39％，P＜0.001。轉染後高錶達OPN的SW-480細胞，GJIC功能被抑製，細胞之間通信減弱，淬滅後恢複率相比較對照組明顯降低。結論 OPN減弱大腸癌細胞間的通信能力，可能與大腸癌細胞的侵襲轉移相關。
목적 검측골교단백( Osteopontin，OPN)전염후대장암SW-480세포주적세포간극련접통신( gap junctional intercellular communication，GJIC)개변。방법 이OPN진핵표체질립전염대장암SW-480세포주후，이용격광공취초현미경（laser scanning confocal microscope，LSCM），운용형광쉬멸후회복기술（flurescence redistribution after photobleaching，FRAP）검측OPN전염후SW-480세포적GJIC공능변화。결과 전염OPN후，고표체OPN적SW-480-pcDNA3.1(+)-OPN세포주쉬멸5 min 후형광회복솔위24.65％±4.08％，이대조조SW-480-pcDNA3.1(+)위44.74％ ±6.23％，P＜0.001；10 min후형광회복솔SW-480-pcDNA3.1(+)-OPN부위25.98％±4.48％，SW-480-pcDNA3.1(+)이상승지64.92％±5.39％，P＜0.001。전염후고표체OPN적SW-480세포，GJIC공능피억제，세포지간통신감약，쉬멸후회복솔상비교대조조명현강저。결론 OPN감약대장암세포간적통신능력，가능여대장암세포적침습전이상관。
ObjectiveLiver metastasis is a major cause of mortality in patients with colorectal cancer. However, the mechanisms underlying this process remain largely unknown. Osteopontin (OPN) is a secreted phosphorylated glycoprotein known to be involved in tumor migration and metastasis. Gap junction intercellular communication (GJIC) is a pathway of direct cell to cell communication. This study analyzed gap junctional intercellular communication(GJIC)changes in colon cancer SW-480 cell lines after osteopontin had been transfected in. MethodsTransfect sense-osteopontin eukaryotic expression plasmids into SW-480 cell lines and detect the GJIC changes in two different cell lines by flurescence redistribution after photobleaching ( FRAP).ResultsCompared with control, signal exchange is impaired and GJIC function is inhibited in SW-480-pcDNA3. 1 ( + )-OPN cell lines. FRAP after 5 min in SW-48O-pcDNA3. 1 ( + )-OPN is 24. 65％ ± 4. 08％, the control SW-480-pcDNA3. 1 ( + ) is 44. 74％ ± 6. 23％, P ＜ 0. 001 ;FRAP after 10 min in SW-480-pcDNA3. 1 ( + )-OPN is 25.98％ ±4.48％, while SW-480-pcDNA3. 1 is up to 64. 92％ ± 5.39％, P ＜ 0.001. ConclusionOPN could inhibit GJIC function of colon cells to accelerate the metastatic process in colorectal cancer.
