CAJ | 학술논문

从高粱(Sorghum bicolor L. var.R1ll)幼苗中提取总RNA,利用RT-PCR和cDNA的3'末端的快速扩增方法(3'RACE),第一次克隆了高粱隐花色素2基因(CRY2)的cDNA序列.该序列包括了一个完整的开放阅读框,编码大小为690个氨基酸残基的蛋白质,与水稻、番茄和拟南芥CRY2蛋白质的同源性分别为8 7%、5 7%和45.5%.高粱CRY2基因组DNA含有3个内含子和4个外显子.RT-PCR检测结果表明,高粱CRY2基因在根、茎和叶中都有转录.Western blotting结果显示CRY2蛋白在根、茎和叶中表达,并在黑暗中积累,蓝光下降解.高粱CRY2可能在蓝光诱导的幼苗去黄化反应中起作用.
종고량(Sorghum bicolor L. var.R1ll)유묘중제취총RNA,이용RT-PCR화cDNA적3'말단적쾌속확증방법(3'RACE),제일차극륭료고량은화색소2기인(CRY2)적cDNA서렬.해서렬포괄료일개완정적개방열독광,편마대소위690개안기산잔기적단백질,여수도、번가화의남개CRY2단백질적동원성분별위8 7%、5 7%화45.5%.고량CRY2기인조DNA함유3개내함자화4개외현자.RT-PCR검측결과표명,고량CRY2기인재근、경화협중도유전록.Western blotting결과현시CRY2단백재근、경화협중표체,병재흑암중적루,람광하강해.고량CRY2가능재람광유도적유묘거황화반응중기작용.
CRY2 is the apoprotein of cryptochrome 2 and CRY2 cDNA has been cloned from Sorghum bicolor in this study. The results of sequence analysis shows that it contains a complete open reading frame encoding a predicted protein of 690 amino acids sharing 87% identity with the CRY2 of rice, but only 45.5% with that of Arabidopsis and 57% with that of tomato. The DNA sequence of sorghum CRY2 contains three introns and four exons. RT-PCR results show that CRY2 has been transcribed in root, leaf and mesocotyl in sorghum seedlings. CRY2 protein has been shown by Western blot analysis to be present in mesocotyl, leaf and root, and to be accumulated in the dark and to be degraded under blue light.