中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
14期
212-213,插图14-8
,共3页
许卫红%林建华%吴朝阳%王长升
許衛紅%林建華%吳朝暘%王長升
허위홍%림건화%오조양%왕장승
骨/损伤%干细胞%骨髓细胞%细胞培养
骨/損傷%榦細胞%骨髓細胞%細胞培養
골/손상%간세포%골수세포%세포배양
背景:迄今为止临床上对较大范围骨缺损的修复仍未得到有效的解决.目的:探讨自体骨髓基质干细胞修复兔桡骨远端骨缺损的可行性.设计:以实验动物为研究对象,自身对照设计的探索性研究.单位:一所医科大学医院骨科.对象:实验于2002-10/2003-04在福建省医科所实验动物中心完成.健康新西兰大白兔12只,雌雄各半,体质量3.1~3.4 kg.方法:取兔髂骨骨髓,体外分离培养扩增骨髓基质干细胞,与生物材料混合后植入体内修复兔桡骨远端骨缺损,应用X射线片、大体标本、组织切片观察修复效果.主要观察指标:主要结局:①大体标本及X射线片.②组织学观察.次要结局:骨髓基质干细胞分离与培养.结果:移植后12周,X射线片显示高密度新骨形成,大体标本上见白色皮质骨充满骨缺损处,组织切片显示骨缺损修复处骨小梁丰富,骨髓腔形成.结论:自体骨髓基质干细胞移植是修复兔桡骨远端骨缺损的理想方法.
揹景:迄今為止臨床上對較大範圍骨缺損的脩複仍未得到有效的解決.目的:探討自體骨髓基質榦細胞脩複兔橈骨遠耑骨缺損的可行性.設計:以實驗動物為研究對象,自身對照設計的探索性研究.單位:一所醫科大學醫院骨科.對象:實驗于2002-10/2003-04在福建省醫科所實驗動物中心完成.健康新西蘭大白兔12隻,雌雄各半,體質量3.1~3.4 kg.方法:取兔髂骨骨髓,體外分離培養擴增骨髓基質榦細胞,與生物材料混閤後植入體內脩複兔橈骨遠耑骨缺損,應用X射線片、大體標本、組織切片觀察脩複效果.主要觀察指標:主要結跼:①大體標本及X射線片.②組織學觀察.次要結跼:骨髓基質榦細胞分離與培養.結果:移植後12週,X射線片顯示高密度新骨形成,大體標本上見白色皮質骨充滿骨缺損處,組織切片顯示骨缺損脩複處骨小樑豐富,骨髓腔形成.結論:自體骨髓基質榦細胞移植是脩複兔橈骨遠耑骨缺損的理想方法.
배경:흘금위지림상상대교대범위골결손적수복잉미득도유효적해결.목적:탐토자체골수기질간세포수복토뇨골원단골결손적가행성.설계:이실험동물위연구대상,자신대조설계적탐색성연구.단위:일소의과대학의원골과.대상:실험우2002-10/2003-04재복건성의과소실험동물중심완성.건강신서란대백토12지,자웅각반,체질량3.1~3.4 kg.방법:취토가골골수,체외분리배양확증골수기질간세포,여생물재료혼합후식입체내수복토뇨골원단골결손,응용X사선편、대체표본、조직절편관찰수복효과.주요관찰지표:주요결국:①대체표본급X사선편.②조직학관찰.차요결국:골수기질간세포분리여배양.결과:이식후12주,X사선편현시고밀도신골형성,대체표본상견백색피질골충만골결손처,조직절편현시골결손수복처골소량봉부,골수강형성.결론:자체골수기질간세포이식시수복토뇨골원단골결손적이상방법.
BACKGROUND: There is no effective measure to repair the large scope bone defect in clinic till now.OBJECTIVE: To explore the feasibility on repairing bone defect of rabbit radius by autologous bone marrow stem cells.DESIGN: Self-controlled groping study focused on experimental animals.SETTING: Department of orthopaedics of a university hogpital.MATERIALS: The study was completed in the Experimental Animal Centre of Fujian Medical Science Institute. Twelve healthy New Zealand rabbits with half sex each were selected with body mass of 3.1 to 3.4 kg.METHODS: The marrow of rabbit ilium was collected and separated, cultured in vitro to prolifeiate bone marrow stromal stem cells and then mixed with biological materials to transplant the distal bone defect area of rabbit radius. X-ray, gross samples and histological section were used to observe the repairing effects.ture of bone marrow stromal stem cellsRESULTS: X-ray showed that new bone with high density was formed 12weeks after transplant and white cortical bone was seen in the defected area from gross sample while histological sections showed that there were plenty of bone trabecules and the marrow cavity was formed.CONCLUSION: Transplant of autogenous bone marrow stromal stem cells is an ideal method to repair distal bone defect of rabbit radius.