CAJ | 학술논문

目的:评估褪黑素预处理对油酸引起的大鼠急性肺损伤的拮抗作用.方法:24只大鼠随机分为生理盐水组、油酸组和褪黑素预处理组3组,检测大鼠肺泡灌洗蛋白、肺的湿干比重、丙二醛、超氧化物岐化酶水平,并进行肺的病理组织学检查.结果:(1)静脉注射0.15 mL/kg油酸引起肺泡灌洗蛋白、肺的湿干比重显著增高(P＜0.01),病理组织学检查发现有肺出血、肺水肿、肺泡间隔增厚以及肺泡腔出现炎性细胞,导致严重的急性肺损伤; (2)提前60 min腹腔注射20 mg/kg褪黑素明显减轻上述的症状,与丙二醛的降低(P＜0.01)和超氧化物岐化酶的升高(P＜0.01)相一致.结论:褪黑素预处理可通过清除和防止自由基的形成,进一步降低肺泡毛细血管膜通透性的增加,从而减轻油酸引起的大鼠急性肺损伤.提示褪黑素可能在急性肺损伤的防治中具有效的.
목적:평고퇴흑소예처리대유산인기적대서급성폐손상적길항작용.방법:24지대서수궤분위생리염수조、유산조화퇴흑소예처리조3조,검측대서폐포관세단백、폐적습간비중、병이철、초양화물기화매수평,병진행폐적병리조직학검사.결과:(1)정맥주사0.15 mL/kg유산인기폐포관세단백、폐적습간비중현저증고(P＜0.01),병리조직학검사발현유폐출혈、폐수종、폐포간격증후이급폐포강출현염성세포,도치엄중적급성폐손상; (2)제전60 min복강주사20 mg/kg퇴흑소명현감경상술적증상,여병이철적강저(P＜0.01)화초양화물기화매적승고(P＜0.01)상일치.결론:퇴흑소예처리가통과청제화방지자유기적형성,진일보강저폐포모세혈관막통투성적증가,종이감경유산인기적대서급성폐손상.제시퇴흑소가능재급성폐손상적방치중구유효적.
AIM:To assess the protective role of melatonin(MEL)in a rat model of oleic-induced acute lung injury.METHODS:Twenty-four rats were randomly allocated to three groups as follows:saline(NS)injection group,oleic acid(OA)injection group and MEL plus OA injection group,the lavage protein,lung wet-to-dry weight ratio,malondialdehyde(MDA)content,superoxide dismutase(SOD)activity and lung histopathology were examined.RESULTS:(1)Injection 0.15 mL/kg of OA led to a severe acute lung injury(ALI),characterized by significantly increasing in lavage protein,lung coefficient(P＜0.01),and by histopathological alterations which presented hemorrhage,edema.thickened alveolar septum and the existence of inflammatory cells in alveolar spaces;(2)Infusion of MEL(20 mg/kg,intraperitoneally for 60 min before the oleic acid)markedly alleviated above-mentioned symptom induced by OA,consistent with decrease of MDA level(P＜0.01) and the increase of SOD activty(P＜0.01).CONCLUSION:Pre-treatment with MEL can attenuate the OA -induced ALI in rats via cleaning and preventing the formation of free radicals and further lessening the increase of alveolocapillary membrane permeability,these data suggest that MEL may be effective in the prevention of ALI.