农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2009年
3期
39-42,71
,共5页
庄新杰%段亚苹%黄怡%宁淑芳%张明
莊新傑%段亞蘋%黃怡%寧淑芳%張明
장신걸%단아평%황이%저숙방%장명
Buffalo%Endometrial epithelial cell%stromal cell%Immunocytochemistry
[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.