白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2011年
9期
550-553
,共4页
范传波%王朝晖%王磊%胡锴勋%刘丽辉%孙琪云%边莉%乌庆超
範傳波%王朝暉%王磊%鬍鍇勛%劉麗輝%孫琪雲%邊莉%烏慶超
범전파%왕조휘%왕뢰%호개훈%류려휘%손기운%변리%오경초
间充质干细胞%猕猴属%免疫
間充質榦細胞%獼猴屬%免疫
간충질간세포%미후속%면역
Mesenchymal stem cells%Macaca%Immunity
目的 评价猕猴间充质干细胞( MSC)静脉异体输注后对细胞免疫功能的影响。方法分离培养MSC;不做其他处理,将异体MSC静脉输注给受者猴,通过定期监测外周血象、混合淋巴细胞反应( MLR)、T细胞亚群来判断MSC输注后受者细胞免疫功能的变化。结果成功培养了猕猴的MSC。异体MSC输注后,受者无明显毒性反应、排异表现及血象变化。可以在一定时间内(2周左右)抑制受者T细胞在MLR中的增殖活性,受者猴A2、A3及A4输注MSC的数量分别为4.0×105/kg、1.0×106/kg、2.0×106/kg,在输注后第14天时,MLR的相对反应值(RR)与输注前比较均明显降低,分别从(46.0±2.6)%、( 40.9±2.3)%、(48.3±2.0)%降至(40.4±1.73)%、(33.0±2.1)%、(39.0±1.0)%(F=1O.19,P=0.023;F=2.593,P= 0.013;F= 28.431,P=0.003),输注后第30天时RR均恢复到输注前水平;统计结果显示,抑制程度(△RR)与输注MSC数量呈正相关(F=27.413,P=0.038)。A4是输注MSC数量最多的受者,输注后第14天开始,外周血CD3+、CD3+ CD4+、CD3+CD8+细胞的百分比与输注前相比有所降低,在输注后第30天左右恢复至输注前水平。结论单纯体内输注异体MSC,可以在一定时间内抑制受者T细胞的免疫活性;免疫抑制程度与输注MSC数量呈正相关。MSC特殊的免疫学特性使其具有深远的临床应用价值。
目的 評價獼猴間充質榦細胞( MSC)靜脈異體輸註後對細胞免疫功能的影響。方法分離培養MSC;不做其他處理,將異體MSC靜脈輸註給受者猴,通過定期鑑測外週血象、混閤淋巴細胞反應( MLR)、T細胞亞群來判斷MSC輸註後受者細胞免疫功能的變化。結果成功培養瞭獼猴的MSC。異體MSC輸註後,受者無明顯毒性反應、排異錶現及血象變化。可以在一定時間內(2週左右)抑製受者T細胞在MLR中的增殖活性,受者猴A2、A3及A4輸註MSC的數量分彆為4.0×105/kg、1.0×106/kg、2.0×106/kg,在輸註後第14天時,MLR的相對反應值(RR)與輸註前比較均明顯降低,分彆從(46.0±2.6)%、( 40.9±2.3)%、(48.3±2.0)%降至(40.4±1.73)%、(33.0±2.1)%、(39.0±1.0)%(F=1O.19,P=0.023;F=2.593,P= 0.013;F= 28.431,P=0.003),輸註後第30天時RR均恢複到輸註前水平;統計結果顯示,抑製程度(△RR)與輸註MSC數量呈正相關(F=27.413,P=0.038)。A4是輸註MSC數量最多的受者,輸註後第14天開始,外週血CD3+、CD3+ CD4+、CD3+CD8+細胞的百分比與輸註前相比有所降低,在輸註後第30天左右恢複至輸註前水平。結論單純體內輸註異體MSC,可以在一定時間內抑製受者T細胞的免疫活性;免疫抑製程度與輸註MSC數量呈正相關。MSC特殊的免疫學特性使其具有深遠的臨床應用價值。
목적 평개미후간충질간세포( MSC)정맥이체수주후대세포면역공능적영향。방법분리배양MSC;불주기타처리,장이체MSC정맥수주급수자후,통과정기감측외주혈상、혼합림파세포반응( MLR)、T세포아군래판단MSC수주후수자세포면역공능적변화。결과성공배양료미후적MSC。이체MSC수주후,수자무명현독성반응、배이표현급혈상변화。가이재일정시간내(2주좌우)억제수자T세포재MLR중적증식활성,수자후A2、A3급A4수주MSC적수량분별위4.0×105/kg、1.0×106/kg、2.0×106/kg,재수주후제14천시,MLR적상대반응치(RR)여수주전비교균명현강저,분별종(46.0±2.6)%、( 40.9±2.3)%、(48.3±2.0)%강지(40.4±1.73)%、(33.0±2.1)%、(39.0±1.0)%(F=1O.19,P=0.023;F=2.593,P= 0.013;F= 28.431,P=0.003),수주후제30천시RR균회복도수주전수평;통계결과현시,억제정도(△RR)여수주MSC수량정정상관(F=27.413,P=0.038)。A4시수주MSC수량최다적수자,수주후제14천개시,외주혈CD3+、CD3+ CD4+、CD3+CD8+세포적백분비여수주전상비유소강저,재수주후제30천좌우회복지수주전수평。결론단순체내수주이체MSC,가이재일정시간내억제수자T세포적면역활성;면역억제정도여수주MSC수량정정상관。MSC특수적면역학특성사기구유심원적림상응용개치。
Objective To study the changes of cellular immunity caused by intravenous infusion of allogenic rhesus mesenchymal stem cells (MSCs). Methods MSCs were isolated and cultured. Then the immunomodulatory effects after MSCs infusion were evaluated by means of peripheral blood counts, mixed lymphocyte reaction (MLR) and analysis of lymphocytic subgroup. Results MSCs of rehsus were successfully cultivated. No acute toxicities or GVHD were observed in recipients. No obvious changes of peripheral blood counts were present. Recipients A2, A3, A4 were administered with MSC by 4.0 ×105/kg, 1.0 ×106/kg, 2.0×106/kg respectively and relative reaction (RR) of MLR decreased 14 days post MSCs infusion: from 46±2.6 %to 40.4±1.73 % (F =10.19, P =0.023), from (40.9±2.3) % to (33±2.1) % (F =2.593, P =0.013), from 48.3±2.0 % to 39±1.0 % (F =28.431, P =0.003) respectively. The decrease degree (ARR) was positively related to the amount of MSCs(F =27.413, P =0.038). RR was restored within 30 days post MSCs infusion. After MSCs infusion, CD3+ CD3+CD4+ and CD3+CD8+ T-lymphocytes decreased in recipient A4, who was administered with the largest number of MSCs, and restored within 30 days. Conclusion MSCs infusion without any other treatment could temporarily inhibit immunity of T lymphocytes in MLR and the immunity inhibition was positively related to the amount of MSCs. The specific immunological characteristics of MSCs were demonstrated with extensive prospect in clinical research.