CAJ | 학술논문

目的比较3H-TdR与125Ⅰ-UdR掺入淋巴细胞的增殖效应.方法用3H-TdR与125Ⅰ-UdR掺入法测定淋巴细胞和Daudi淋巴瘤细胞的增殖效应.结果 3H-TdR和125Ⅰ-UdR在正常淋巴细胞中的掺入率分别为20.95%±1.06%和1.00%±0.04%,在Daudi淋巴瘤细胞中的掺入率分别为29.94%±4.10%和6.02%±0.73%.3H-TdR在细胞中的掺入率明显高于125Ⅰ-UdR;且3H-TdR和125Ⅰ-UdR在淋巴瘤细胞中的掺入率高于正常淋巴细胞.结论就淋巴细胞而言,作为示踪剂125Ⅰ-UdR不能替代3H-TdR;但对于淋巴瘤细胞,能否代替3H-TdR有待于进一步研究.
목적 비교3H-TdR여125Ⅰ-UdR참입림파세포적증식효응.방법 용3H-TdR여125Ⅰ-UdR참입법측정림파세포화Daudi림파류세포적증식효응.결과 3H-TdR화125Ⅰ-UdR재정상림파세포중적참입솔분별위20.95%±1.06%화1.00%±0.04%,재Daudi림파류세포중적참입솔분별위29.94%±4.10%화6.02%±0.73%.3H-TdR재세포중적참입솔명현고우125Ⅰ-UdR;차3H-TdR화125Ⅰ-UdR재림파류세포중적참입솔고우정상림파세포.결론 취림파세포이언,작위시종제125Ⅰ-UdR불능체대3H-TdR;단대우림파류세포,능부대체3H-TdR유대우진일보연구.
Objective To compare the incorporation method of 3H-TdR and 125Ⅰ-UdR on determining the proliferation effect of lymphocytes. Methods The proliferation effects of lymphocyte and Daudi lymphoma cells were estimated by 3H-TdR and 125Ⅰ-UdR incorporation. Results The incorporating fraction of 3H-TdR and 125Ⅰ-UdR into lymphocyte was 20.95% ± 1.06% and 1.00% ±0.04%,respectively, and the incorporating fraction for the lymphoma cells was 29. 94% ± 4. 10% and 6. 02% ±0. 73% ,respectively. The incorporation fractions of 3H-TdR into lymphocyte and lymphoma cells were much higher than those of 125Ⅰ-UdR, but the incorporating fractions of 3H-TdR or 125Ⅰ-UdR into the lymphoma cells were much higher than those of lymphocytes. Conclusions For lymphocytes, 125Ⅰ-UdR cannot substitute 3H-TdR as a tracer agent. But for lymphoma cells, whether 125Ⅰ-UdR could be replace 3H-TdR or not needs further research.