中国医药
中國醫藥
중국의약
CHINA MEDICINE
2009年
2期
84-86
,共3页
于金玲%张成明%张岩%赵恒珂%王洪静
于金玲%張成明%張巖%趙恆珂%王洪靜
우금령%장성명%장암%조항가%왕홍정
镇痛%大鼠%布托啡诺%白细胞介素6%白细胞介素-10
鎮痛%大鼠%佈託啡諾%白細胞介素6%白細胞介素-10
진통%대서%포탁배낙%백세포개소6%백세포개소-10
Analgesia%Rat%Butorphanol%Interleukin-6%Interleukin-10
目的 研究鞘内注射布托啡诺对大鼠急性疼痛时血清细胞因子水平的影响,探讨鞘内注射布托啡诺治疗急性疼痛的机制.方法 取清洁级SD雄性成年大鼠45只,随机数字表法分为空白组、对照组与布托啡诺组,每组15只;每组再平均分为3个亚组,每亚组5只.空白组不实施任何手术;对照组及布托啡诺组制造慢性缩窄性损伤模型及鞘内置管后,分别鞘内注射生理盐水10 μl及布托啡诺24 μg.3组分别自内眦取血,双抗体夹心酶联免疫吸附法检测血清白细胞介素(IL)-6与IL-10水平.结果 术后48 h内对照组和布托啡诺组IL-6水平均高于术前,差异有统计学意义(P<0.05或P<0.01);对照组IL-6在术后6 h达峰值[(114.35±9.04)ng/L],而布托啡诺组的峰值[(40.55±5.97)ng/L]延迟到术后12 h,且布托啡诺组各时点的IL-6均低于对照组,差异有统计学意义(均P<0.01).对照组血清IL-10水平在术后12 h开始高于术前,布托啡诺组则均在术后9 h开始高于术前,且术后9～48 h各时点布托啡诺组IL-10水平均高于对照组,差异有统计学意义(均P<0.01).对照组血清IL-10水平在术后18 h达峰值[(29.53±3.14)ng/L],布托啡诺组血清IL-10水平在术后15 h达峰值[(50.39±2.74)ng/L].结论 鞘内注射布托啡诺可降低IL-6水平,并明显增高IL-10水平,并阻断IL-6恶性循环,延缓疼痛高峰时相及增高疼痛阈值,促进促/抑炎症细胞因子平衡的恢复.
目的 研究鞘內註射佈託啡諾對大鼠急性疼痛時血清細胞因子水平的影響,探討鞘內註射佈託啡諾治療急性疼痛的機製.方法 取清潔級SD雄性成年大鼠45隻,隨機數字錶法分為空白組、對照組與佈託啡諾組,每組15隻;每組再平均分為3箇亞組,每亞組5隻.空白組不實施任何手術;對照組及佈託啡諾組製造慢性縮窄性損傷模型及鞘內置管後,分彆鞘內註射生理鹽水10 μl及佈託啡諾24 μg.3組分彆自內眥取血,雙抗體夾心酶聯免疫吸附法檢測血清白細胞介素(IL)-6與IL-10水平.結果 術後48 h內對照組和佈託啡諾組IL-6水平均高于術前,差異有統計學意義(P<0.05或P<0.01);對照組IL-6在術後6 h達峰值[(114.35±9.04)ng/L],而佈託啡諾組的峰值[(40.55±5.97)ng/L]延遲到術後12 h,且佈託啡諾組各時點的IL-6均低于對照組,差異有統計學意義(均P<0.01).對照組血清IL-10水平在術後12 h開始高于術前,佈託啡諾組則均在術後9 h開始高于術前,且術後9～48 h各時點佈託啡諾組IL-10水平均高于對照組,差異有統計學意義(均P<0.01).對照組血清IL-10水平在術後18 h達峰值[(29.53±3.14)ng/L],佈託啡諾組血清IL-10水平在術後15 h達峰值[(50.39±2.74)ng/L].結論 鞘內註射佈託啡諾可降低IL-6水平,併明顯增高IL-10水平,併阻斷IL-6噁性循環,延緩疼痛高峰時相及增高疼痛閾值,促進促/抑炎癥細胞因子平衡的恢複.
목적 연구초내주사포탁배낙대대서급성동통시혈청세포인자수평적영향,탐토초내주사포탁배낙치료급성동통적궤제.방법 취청길급SD웅성성년대서45지,수궤수자표법분위공백조、대조조여포탁배낙조,매조15지;매조재평균분위3개아조,매아조5지.공백조불실시임하수술;대조조급포탁배낙조제조만성축착성손상모형급초내치관후,분별초내주사생리염수10 μl급포탁배낙24 μg.3조분별자내자취혈,쌍항체협심매련면역흡부법검측혈청백세포개소(IL)-6여IL-10수평.결과 술후48 h내대조조화포탁배낙조IL-6수평균고우술전,차이유통계학의의(P<0.05혹P<0.01);대조조IL-6재술후6 h체봉치[(114.35±9.04)ng/L],이포탁배낙조적봉치[(40.55±5.97)ng/L]연지도술후12 h,차포탁배낙조각시점적IL-6균저우대조조,차이유통계학의의(균P<0.01).대조조혈청IL-10수평재술후12 h개시고우술전,포탁배낙조칙균재술후9 h개시고우술전,차술후9～48 h각시점포탁배낙조IL-10수평균고우대조조,차이유통계학의의(균P<0.01).대조조혈청IL-10수평재술후18 h체봉치[(29.53±3.14)ng/L],포탁배낙조혈청IL-10수평재술후15 h체봉치[(50.39±2.74)ng/L].결론 초내주사포탁배낙가강저IL-6수평,병명현증고IL-10수평,병조단IL-6악성순배,연완동통고봉시상급증고동통역치,촉진촉/억염증세포인자평형적회복.
Objective To measure serum IL-6 and IL-10 in acute painful rats of intratheeal butorphanol and to investigate theraputic mechanisms of butorphanol on acute pain. Methods Fourty-five healthy male SD rats were randomly divided into three groups. Every group was equally divided into three subgroups with5 animals in each subgroup. Group B accepted the same basic anesthesia without any operation and blood was obtained from epieathus of rats. Group C received intrathecal normal saline 10 μl after CCI. Group T received intrathecal butorphanol 24 μg after CCI. In subgroup 1 blood samples were drawn from epicathus before the CCI, 9 hours, 18 hours after CCI. Similarly, blood samples in subgroup 2 were obtained at hours 3, 12, 24 while subgroup3 on hour6, 15,48, respectively. Serum IL-6 and IL-10 in each sample were measured by enzyme linked immunosorbent assay. Results Serum IL-6 in group C and group T within 48 hours after CCI increased after operation. In group C, the peak time of serum IL-6 was 6 hours after operation with 12 hours in group T. The serum IL-6 in group T were lower than that in group C at the same time point. Serum IL-10 in group C began to increase 12 hours after CCI. Serum IL-IO production in group C reached peak value at 18h after CCI, with 15h in group T. Conclusion Intratheeal butorphanol decreases the level of serum IL-6 and increases serum IL-10 ,accelerating the balance of promotion or inhibitor inflammatory factors.
