中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2009年
11期
837-842
,共6页
富小红%孟立强%唐嘉薇%屈磊%李晓玫
富小紅%孟立彊%唐嘉薇%屈磊%李曉玫
부소홍%맹립강%당가미%굴뢰%리효매
黄芪%当归%NADPH氧化酶%硝基酪氨酸%纤维化
黃芪%噹歸%NADPH氧化酶%硝基酪氨痠%纖維化
황기%당귀%NADPH양화매%초기락안산%섬유화
Astragalus membranaceus%Angelica sinensis%NADPH oxidase%Nitrotyrosine%Fibrosis
目的 利用梗阻性肾病大鼠模型探讨黄芪当归合剂(A&A)对肾间质纤维化过程中活性氧生成和清除的影响及其相关机制.方法 雄性Wistar大鼠随机分为对照、假手术(Sham)、模型(UUO)和模型+A&A治疗(UAA)组.UAA组给予A&A(相当于黄苠和当归生药各14 g·kg~(-1)·d~(-1))灌胃,Sham和UUO组给予等量清水.造模后第3天观察肾脏组织标本病理损伤程度;分光光度法测定肾组织匀浆总抗氧化能力(T-AOC)、CuZn超氧化物歧化酶(CuZn.SOD)活力;Western印迹方法分析肾组织中NADPH氧化酶亚基p47-phox、p22-phox及硝基酪氨酸的表达.结果 UUO组出现严重间质炎性细胞浸润伴轻微肾小管萎缩及间质纤维化,A&A减轻了肾脏病理损伤.与Sham组相比,UUO组T-AOC无明显变化,但硝基酪氨酸、NADPH氧化酶亚基p47-phox和p22-phox表达显著上调(P<0.05).与UUO组相比,UAA组T-AOC表达上调(2.5±1.1比1.5±0.5,P<0.05);硝基酪氨酸表达下调(P<0.05);NADPH氧化酶p47-phox、p22-phox表达均下调(均P<0.05);而CuZn-SOD无明显变化.结论 梗阻性肾病中,A&A通过抑制肾组织NADPH氧化酶亚基p47-phox和p22-phox表达,减少活性氧的生成;减轻肾组织氧化损伤;抑制肾间质纤维化.
目的 利用梗阻性腎病大鼠模型探討黃芪噹歸閤劑(A&A)對腎間質纖維化過程中活性氧生成和清除的影響及其相關機製.方法 雄性Wistar大鼠隨機分為對照、假手術(Sham)、模型(UUO)和模型+A&A治療(UAA)組.UAA組給予A&A(相噹于黃苠和噹歸生藥各14 g·kg~(-1)·d~(-1))灌胃,Sham和UUO組給予等量清水.造模後第3天觀察腎髒組織標本病理損傷程度;分光光度法測定腎組織勻漿總抗氧化能力(T-AOC)、CuZn超氧化物歧化酶(CuZn.SOD)活力;Western印跡方法分析腎組織中NADPH氧化酶亞基p47-phox、p22-phox及硝基酪氨痠的錶達.結果 UUO組齣現嚴重間質炎性細胞浸潤伴輕微腎小管萎縮及間質纖維化,A&A減輕瞭腎髒病理損傷.與Sham組相比,UUO組T-AOC無明顯變化,但硝基酪氨痠、NADPH氧化酶亞基p47-phox和p22-phox錶達顯著上調(P<0.05).與UUO組相比,UAA組T-AOC錶達上調(2.5±1.1比1.5±0.5,P<0.05);硝基酪氨痠錶達下調(P<0.05);NADPH氧化酶p47-phox、p22-phox錶達均下調(均P<0.05);而CuZn-SOD無明顯變化.結論 梗阻性腎病中,A&A通過抑製腎組織NADPH氧化酶亞基p47-phox和p22-phox錶達,減少活性氧的生成;減輕腎組織氧化損傷;抑製腎間質纖維化.
목적 이용경조성신병대서모형탐토황기당귀합제(A&A)대신간질섬유화과정중활성양생성화청제적영향급기상관궤제.방법 웅성Wistar대서수궤분위대조、가수술(Sham)、모형(UUO)화모형+A&A치료(UAA)조.UAA조급여A&A(상당우황민화당귀생약각14 g·kg~(-1)·d~(-1))관위,Sham화UUO조급여등량청수.조모후제3천관찰신장조직표본병리손상정도;분광광도법측정신조직균장총항양화능력(T-AOC)、CuZn초양화물기화매(CuZn.SOD)활력;Western인적방법분석신조직중NADPH양화매아기p47-phox、p22-phox급초기락안산적표체.결과 UUO조출현엄중간질염성세포침윤반경미신소관위축급간질섬유화,A&A감경료신장병리손상.여Sham조상비,UUO조T-AOC무명현변화,단초기락안산、NADPH양화매아기p47-phox화p22-phox표체현저상조(P<0.05).여UUO조상비,UAA조T-AOC표체상조(2.5±1.1비1.5±0.5,P<0.05);초기락안산표체하조(P<0.05);NADPH양화매p47-phox、p22-phox표체균하조(균P<0.05);이CuZn-SOD무명현변화.결론 경조성신병중,A&A통과억제신조직NADPH양화매아기p47-phox화p22-phox표체,감소활성양적생성;감경신조직양화손상;억제신간질섬유화.
Objective To investigate the influence of a combination of Chinese herbs,Astragalus membranaceus var.mongholicus and A ngelica sinensis (A&A)on the production and elimination of reactive oxygen species (ROS)and the underlying mechanism during the process of renal interstitial fibrosis in the obstructive kidneys of rats with unilateral ureteral obstruction(uuo).Method Male Wistar rats were randomly divided into control,sham,UUO and UAA (UUO+A&A)groups.The rats in UAA group were administered with A&A(14 g/kg)by oral gavage once daily:the ones in sham and UUO group were given with equal volumes of water.Three days after setting up models,pathological injury of renal tissue was evaluated.Level of total antioxidant capacity(T-AOC)and activity of CuZn superoxide dismutase(CuZn-SOD)in renal homogenates were measured bv spectrophotometry.Expression of NADPH oxidase subunits p47-phox,p22-phox and nintrotyrosine was analyzed by Western blot. Results Severe interstitial inflammatory cells infiltration, mild tubular atrophy and interstitial fibrosis were found in UUO group, which were alleviated by A&A administration. Compared to sham group, T-AOC of UUO group was not significantly changed, but the expression of the nitrotyrosine, NADPH oxidase subunits p47-phox and p22-phox was increased significantly (P<0.05). After A&A administration, T-AOC level was increased (2.5±1.1 vs 1.5±0.5, P<0.05) and the expression of nitrotyrosine was decreased (P< 0.05) in UAA group compared with UUO group. Additionally, the expression of p47-phox was reduced at day 3 (P<0.05), accompanied with a reduced expression of p22-phox (P< 0.05). CuZn-SOD activity was not significantly changed among the groups. Conclusion The inhibition of A&A in NADPH oxidase subunits p47-phox and p22-phox, which is responsible for reduction of oxidative stress, is associated with the alleviation of renal fibrosis in obstructive rat kidney.
