CAJ | 학술논문

目的:观察大豆异黄酮对心肌梗死大鼠心室重构的影响.方法:雄性SD大鼠,结扎冠状动脉左前降支,造成心肌梗死.实验分为6组:假手术组,心肌梗死模型组,卡托普利组,大豆异黄酮低、中、高剂量组.在结扎冠状动脉后3 h开始,分别灌胃给予0.5%羧甲基纤维素钠(CMC-Na),0.5%CMC-Na,卡托普利50 mg·kg-1和大豆异黄酮30,90,270 mg·kg-1,qd,共35 d.给药容积均为10 mL·kg-1.最后一次给药后24 h,测定心率(HR)、左室舒张末期压(LVEDP)、左室收缩压峰值(Peak)、左室压最大上升速率/下降速率(±dp/dtmax)和心肌最大缩短速率(Vmax),测定全心室质量和体重比(TVW/BW),用天狼猩红染色,在图像分析系统下测量梗死范围(IS)、左心室内径(LVD)、室间隔厚度(ST),心肌间质胶原容积系数(ICVF)和血管周围胶原容积系数(PCVF).结果:卡托普利、大豆异黄酮90 mg·kg-1和270 mg·kg-1组大鼠的梗死范围分别是(31±s 4)%,(32±3)%和(31±5)%,均显著小于心肌梗死模型组大鼠[(38.9±2.9)%,P＜0.01].心肌梗死组大鼠的TVW/BW,LVD,ICVF和PCVF均比假手术组明显增加(P＜0.01),增加的TVW/BW,LVD,ICVF和PCVF用卡托普利和大豆异黄酮90 mg·kg-1,270 mg·kg-1治疗后明显减少[P＜0.01,P＜0.05(SI 90 mg·kg-1组的LVD)].心肌梗死大鼠的ST显著减小(P＜0.01),而给予卡托普利和大豆异黄酮可增加ST(P＜0.01).结扎LAD后,大鼠的Peak,±dp/dtmax和Vmax明显降低(P＜0.01),而LVEDP明显升高(P＜0.01),降低的Peak,±dp/dtmax和Vmax,在给予卡托普利、大豆异黄酮90 mg·kg-1和270 mg·kg-1后明显升高(P＜0.01);而升高的LVEDP仅在给予卡托普利治疗后降低.结论:大豆异黄酮能够改善心肌梗死大鼠的心功能和心室重构.
목적:관찰대두이황동대심기경사대서심실중구적영향.방법:웅성SD대서,결찰관상동맥좌전강지,조성심기경사.실험분위6조:가수술조,심기경사모형조,잡탁보리조,대두이황동저、중、고제량조.재결찰관상동맥후3 h개시,분별관위급여0.5%최갑기섬유소납(CMC-Na),0.5%CMC-Na,잡탁보리50 mg·kg-1화대두이황동30,90,270 mg·kg-1,qd,공35 d.급약용적균위10 mL·kg-1.최후일차급약후24 h,측정심솔(HR)、좌실서장말기압(LVEDP)、좌실수축압봉치(Peak)、좌실압최대상승속솔/하강속솔(±dp/dtmax)화심기최대축단속솔(Vmax),측정전심실질량화체중비(TVW/BW),용천랑성홍염색,재도상분석계통하측량경사범위(IS)、좌심실내경(LVD)、실간격후도(ST),심기간질효원용적계수(ICVF)화혈관주위효원용적계수(PCVF).결과:잡탁보리、대두이황동90 mg·kg-1화270 mg·kg-1조대서적경사범위분별시(31±s 4)%,(32±3)%화(31±5)%,균현저소우심기경사모형조대서[(38.9±2.9)%,P＜0.01].심기경사조대서적TVW/BW,LVD,ICVF화PCVF균비가수술조명현증가(P＜0.01),증가적TVW/BW,LVD,ICVF화PCVF용잡탁보리화대두이황동90 mg·kg-1,270 mg·kg-1치료후명현감소[P＜0.01,P＜0.05(SI 90 mg·kg-1조적LVD)].심기경사대서적ST현저감소(P＜0.01),이급여잡탁보리화대두이황동가증가ST(P＜0.01).결찰LAD후,대서적Peak,±dp/dtmax화Vmax명현강저(P＜0.01),이LVEDP명현승고(P＜0.01),강저적Peak,±dp/dtmax화Vmax,재급여잡탁보리、대두이황동90 mg·kg-1화270 mg·kg-1후명현승고(P＜0.01);이승고적LVEDP부재급여잡탁보리치료후강저.결론:대두이황동능구개선심기경사대서적심공능화심실중구.
AIM: To investigate the effect of soybean isoflavones (SI) on ventricular remodeling induced by myocardial infarction (MI). METHODS: MI was induced by permanent ligation of the left anterior descending coronary artery (LAD) in male Sprague-Dawley (SD)rats. Rats were randomly divided into six groups: shamThree hours after the operation, the drugs or solvent were administrated ig qd for 35 d. Twenty-four hours after the last administration, heart rate (HR), left ventricular end-diastolic pressure (LVEDP), peak systolic left ventricular pressure (Peak), maximal rate of left ventricular pressure rising (+dp/dtmax), maximal rate of left ventricular pressure declining (-dp/dtmax) and myocardial maximal shortening velocity (Vmax) were measured. After above hemodynamic parameters were measured, hearts were extracted. The ratio of total ventricle weight to body weight(TVW/BW) was calculated. Myocardial collagen was shown with the collagen-specific picrosirius red stain,myocardial interstitial collagen volume fraction(ICVF), perivascular collagen volume fraction(PCVF), infarct size,septal thickness (ST) and left ventricular diameter (LVD) were measured by image analysis system. RESULTS:(31 ± 5) %, respectively, all of which were significantly smaller than that in MI group (( 38.9 ± 2.9) %, P ＜ 0.01 ).In MI group, TVW/BW, LVD, ICVF and PCVF were remarkably increased compared with those in sham group (P ＜ 0.01 ). The increased TVW/BW, LVD, ICVF and PCVF were significantly reduced by treatment with eaprats in MI group was significantly decreased than that in sham group (P ＜ 0.01 ). The reduced ST could be in-dp/dtmax and Vmax were decreased (P ＜ 0.01 ), while LVEDP was increased (P ＜ 0.01 ), significantly. The reSION: SI can improve cardiac function and ventricular remodeling induced by myocardial infarction in the rat.