中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2009年
7期
863-865
,共3页
刘林林%郭彩霞%孙宝胜%李艳博%龚守良%怀淑君
劉林林%郭綵霞%孫寶勝%李豔博%龔守良%懷淑君
류림림%곽채하%손보성%리염박%공수량%부숙군
γ干扰素%原核表达载体%分泌型
γ榦擾素%原覈錶達載體%分泌型
γ간우소%원핵표체재체%분비형
IFN-γ%prokaryotic expression vector%secretion type
目的 构建小鼠分泌型γ干扰素(IFN-γ)原核表达载体pFLAG-IFN-γ.方法 利用分子生物学方法,从pcDNA3.1-Egr1-IFN-γ质粒上将IFN-γ切下,然后连接到原核表达载体pFLAG-shift12TM上,构建成pFLAG-IFN-γ表达载体,进行PCR、酶切鉴定.结果 经鉴定,所构建的质粒pFLAG-IFN-γ的鉴定结果与预期的一致.结论 成功地构建了带有分泌信号的原核表达载体pFLAG-IFN-γ.
目的 構建小鼠分泌型γ榦擾素(IFN-γ)原覈錶達載體pFLAG-IFN-γ.方法 利用分子生物學方法,從pcDNA3.1-Egr1-IFN-γ質粒上將IFN-γ切下,然後連接到原覈錶達載體pFLAG-shift12TM上,構建成pFLAG-IFN-γ錶達載體,進行PCR、酶切鑒定.結果 經鑒定,所構建的質粒pFLAG-IFN-γ的鑒定結果與預期的一緻.結論 成功地構建瞭帶有分泌信號的原覈錶達載體pFLAG-IFN-γ.
목적 구건소서분비형γ간우소(IFN-γ)원핵표체재체pFLAG-IFN-γ.방법 이용분자생물학방법,종pcDNA3.1-Egr1-IFN-γ질립상장IFN-γ절하,연후련접도원핵표체재체pFLAG-shift12TM상,구건성pFLAG-IFN-γ표체재체,진행PCR、매절감정.결과 경감정,소구건적질립pFLAG-IFN-γ적감정결과여예기적일치.결론 성공지구건료대유분비신호적원핵표체재체pFLAG-IFN-γ.
Objective To construct the prokaryotic expression vector pFLAG-IFN-γ which contains the mouse secreting type interferon-gamma (IFN-γ).Methods The IFN-γ fragment was acquired from the plasmid pcDNA3.1-Egr1- IFN-γ,and then it was ligated to the prokaryotic expression vector pFLAG-shift12TM which finally constructed the recombinant plasmid pFLAG-IFN-γ.Furthermore,PCR and enzyme digestion were used to identify it.Results The fragments acquired by PCR and enzyme digestion identification were all in concordance with the expected results that indicated that the pFLAG-IFN-γ was correctly constructed.Conclusion The recombinant plasmid pFLAG-IFN-γ which contains mouse secretion type IFN-γ was constructed successfully.
