CAJ | 학술논문

目的探讨肿瘤细胞对光动力的敏感度,同时为体外细胞培养的光动力实验研究选择最佳光剂量和最佳光密度提供实验依据.方法将人永生化食管上皮细胞系SHEE及其癌变细胞系SHEEC分别随机分成21组,接种24 h贴壁后,在30μg/ml的光敏剂photofrin-Ⅱ溶液中孵育150 min后,在15 min内应用Diomed-630型激光仪给予25 mW/cm~2,50 mW/cm~2、100 mw/cm~2 3个功率密度分别照射10,20,30,50,100,150,200 s,然后继续培养24 h,应用缩胆囊胆素八肽(CCK-8)法检测两个细胞系的存活率.结果在photofrin-Ⅱ 30 μg/ml浓度条件下,相同功率密度的光动力疗法(PDT)对细胞系SHEE和SHEEC的抑制率无显著性差异;在photofrin-Ⅱ 30μg/ml浓度条件下,随着光剂量的增加PDT对细胞系SHEE和SHEEC的抑制率呈增加趋势,达到2.5～3.0 J/cm~2后进入平台期.结论人永生化食管上皮细胞系SHEE及其癌变细胞系SHEEC对光动力的敏感度无显著性差异,提示肿瘤光动力治疗的靶向作用可能与肿瘤细胞对激光的敏感性无关.
목적 탐토종류세포대광동력적민감도,동시위체외세포배양적광동력실험연구선택최가광제량화최가광밀도제공실험의거.방법 장인영생화식관상피세포계SHEE급기암변세포계SHEEC분별수궤분성21조,접충24 h첩벽후,재30μg/ml적광민제photofrin-Ⅱ용액중부육150 min후,재15 min내응용Diomed-630형격광의급여25 mW/cm~2,50 mW/cm~2、100 mw/cm~2 3개공솔밀도분별조사10,20,30,50,100,150,200 s,연후계속배양24 h,응용축담낭담소팔태(CCK-8)법검측량개세포계적존활솔.결과 재photofrin-Ⅱ 30 μg/ml농도조건하,상동공솔밀도적광동력요법(PDT)대세포계SHEE화SHEEC적억제솔무현저성차이;재photofrin-Ⅱ 30μg/ml농도조건하,수착광제량적증가PDT대세포계SHEE화SHEEC적억제솔정증가추세,체도2.5～3.0 J/cm~2후진입평태기.결론 인영생화식관상피세포계SHEE급기암변세포계SHEEC대광동력적민감도무현저성차이,제시종류광동력치료적파향작용가능여종류세포대격광적민감성무관.
Objective To investigate the sensitivity of tumor cells to photodynamic therapy(PDT), and to select the optimal photo-dose and photo density for PDT to the cultured cells. Methods Cells of SHEE and SHEEC cell lines were divided into 21 groups randomly,after 24 h inoculation, the cells accreted on paries of culture capsule completely, we replaced M199 complete culture solution with 30 μg/rnl Photofrin-Ⅱ solution 100 μl and then replacd with M199 complete culture solution without Photofiin-Ⅱ after 150 min of incubation in Photofrin-Ⅱ. Within 15 min,we dealed the cells with PDT using Photofrin-Diomed 630 under three different power densities:25 raW/cm~2,50 mW/cm~2 and 100 mW/cm~2 for 10 s,20 9,30 s,50 s, 100 s, 150 s and 200 s respectively,and then continue for 24 h culture. We examined the inhibiting effect on cell line SHEE and SHEEC under PDT by the method of CCK-8. Results There was no significant difference in the inhibition ratio with same power density of PDT between cell line SHEE and SHEEC under the concentration of 30 μg/ml Photofrin-Ⅱ. However, the inhibition ratio increased with the raising of photo-dose of PDT,but there was a platform stage after the 2.5～30 J/cm~ 2 photo-dose. Conclu-sion The difference for photodynamic sensitivity between human immortalization esophageal epithelial cell line SHEE and the malignant transformation cell line SHEEC is not significant. The targeting of PDT to malignant tumor cell may not be involved in the photosensitivity for tumor cell.