CAJ | 학술논문

目的研究一氧化氮合酶(NOS)在犬下颌骨牵张成骨及骨创伤修复过程的表达和意义.方法 28只犬随机分成牵张组和直接延长组各12只及正常对照组4只,用免疫组化法检测牵张第6天、牵张后固定2周和8周NOS表达水平的变化.结果牵张第6天牵张组可见牵开区组织内炎性细胞浸润,血管周围和间质内较多红细胞漏出.牵张及固定早期,牵张组和直接延长组诱导型NOS(iNOS)与内皮型NOS(eNOS)阳性表达均明显高于正常对照组,直接延长组的iNOS和eNOS阳性表达低于牵张组,差异均有统计学意义(P＜0.05);牵张后固定8周iNOS和eNOS阳性表达3组间比较差异无统计学意义(P>0.05).结论 NOS在牵张早期表达升高,结合在牵张早期组织内红细胞漏出,提示牵张成骨过程中存在某种程度的微创伤,这种微创伤可能是牵张成骨的重要启动因素之一.
목적 연구일양화담합매(NOS)재견하합골견장성골급골창상수복과정적표체화의의.방법 28지견수궤분성견장조화직접연장조각12지급정상대조조4지,용면역조화법검측견장제6천、견장후고정2주화8주NOS표체수평적변화.결과 견장제6천견장조가견견개구조직내염성세포침윤,혈관주위화간질내교다홍세포루출.견장급고정조기,견장조화직접연장조유도형NOS(iNOS)여내피형NOS(eNOS)양성표체균명현고우정상대조조,직접연장조적iNOS화eNOS양성표체저우견장조,차이균유통계학의의(P＜0.05);견장후고정8주iNOS화eNOS양성표체3조간비교차이무통계학의의(P>0.05).결론 NOS재견장조기표체승고,결합재견장조기조직내홍세포루출,제시견장성골과정중존재모충정도적미창상,저충미창상가능시견장성골적중요계동인소지일.
Objective To investigate the expression and significance of nitric oxide synthase(NOS)during mandibular distraction osteogenesis and bone trauma healing,to futher study the mechanism of distraction osteogenesis.Methods Twenty-eight adult dogs were randomly divided into DO group(12 dogs),acutely lengthening group(12 dogs)and control group(4dogs).Immunohistochemical examination were carried out to test the expression of NOS during the sixth day in distraction period,the second and eighth week of consolidation.Results In DO group infiltration of inflammatory cell was found in the distraction gap,more red blood cells(RBC)leak out around vascellum and matrix in the sixth day in distraction period.The expression of local iNOS(inducible NOS)and eNOS(endothelinal NOS)in DO gruoup and acutely lengthening group was higher than that in the control group(P<0.05),the expression of local iNOS and eNOS in acutely lengthening group was lower than that in DO group(P<0.05)in the early of distraction period and the consolidation.The expression of local iNOS and eNOS was no statistic difference between three groups(P>0.05)in the eighth week of consolidation.Conclusion NOS as a sensitive index of tissue trauma are highly expressed,and RBC was found leaking out in the early of distraction,indicating that micro-trauma to some extent may occurr during DO procedure,the micro-trauma may be one of the significant factors which increasing regeneration of bone during DO.