癌症
癌癥
암증
CHINESE JOURNAL OF CANCER
2010年
1期
52-58
,共7页
苏铭%黎乐群%彭涛%郭雅%肖开银%尚丽明%徐邦浩%李仕来%苏智雄%叶新平
囌銘%黎樂群%彭濤%郭雅%肖開銀%尚麗明%徐邦浩%李仕來%囌智雄%葉新平
소명%려악군%팽도%곽아%초개은%상려명%서방호%리사래%소지웅%협신평
肝肿瘤%肝内转移%多中心发生%多结节性%蛋白质组%比较蛋白质组学分析
肝腫瘤%肝內轉移%多中心髮生%多結節性%蛋白質組%比較蛋白質組學分析
간종류%간내전이%다중심발생%다결절성%단백질조%비교단백질조학분석
liver neoplasm%intrahepatic metastasis%multicentric occurrence%multinodular hepatocellular carcinoma%proteomics%comparatine proteomicanalysis
背景与目的:多结节肝癌的起源既有单一原发肿瘤的肝内转移,又有各个结节单独存在的多中心性发生.我们对肝内转移组和多中心发生组多结节肝癌组织的全蛋白质表达谱进行比较分析,希望筛选出在多结节肝癌细胞克隆起源过程中发挥重要作用的差异蛋白分子.方法:应用双向凝胶电泳分离肝内转移组及多中心发生组多结节肝癌组织总蛋白,对两组间蛋白差异点应用质谱进行分析及数据库搜素、鉴定.结果:肝内转移组平均检测到(1025±52)个蛋白点(n=3),多中心发生组平均检测到(900±98)个蛋白点(n=3).筛选出差异蛋白点25个,应用质谱鉴定出其中核纤层蛋白A/C异构体2等20种差异蛋白质.结论:多中心发生组与肝内转移组多结节肝癌的蛋白表达谱存在差异.鉴定出的20个差异表达蛋白可能参与肝癌的发生、发展、侵袭、转移,有可能成为潜在的、有应用价值的多结节肝癌细胞克隆起源鉴别分子标志.
揹景與目的:多結節肝癌的起源既有單一原髮腫瘤的肝內轉移,又有各箇結節單獨存在的多中心性髮生.我們對肝內轉移組和多中心髮生組多結節肝癌組織的全蛋白質錶達譜進行比較分析,希望篩選齣在多結節肝癌細胞剋隆起源過程中髮揮重要作用的差異蛋白分子.方法:應用雙嚮凝膠電泳分離肝內轉移組及多中心髮生組多結節肝癌組織總蛋白,對兩組間蛋白差異點應用質譜進行分析及數據庫搜素、鑒定.結果:肝內轉移組平均檢測到(1025±52)箇蛋白點(n=3),多中心髮生組平均檢測到(900±98)箇蛋白點(n=3).篩選齣差異蛋白點25箇,應用質譜鑒定齣其中覈纖層蛋白A/C異構體2等20種差異蛋白質.結論:多中心髮生組與肝內轉移組多結節肝癌的蛋白錶達譜存在差異.鑒定齣的20箇差異錶達蛋白可能參與肝癌的髮生、髮展、侵襲、轉移,有可能成為潛在的、有應用價值的多結節肝癌細胞剋隆起源鑒彆分子標誌.
배경여목적:다결절간암적기원기유단일원발종류적간내전이,우유각개결절단독존재적다중심성발생.아문대간내전이조화다중심발생조다결절간암조직적전단백질표체보진행비교분석,희망사선출재다결절간암세포극륭기원과정중발휘중요작용적차이단백분자.방법:응용쌍향응효전영분리간내전이조급다중심발생조다결절간암조직총단백,대량조간단백차이점응용질보진행분석급수거고수소、감정.결과:간내전이조평균검측도(1025±52)개단백점(n=3),다중심발생조평균검측도(900±98)개단백점(n=3).사선출차이단백점25개,응용질보감정출기중핵섬층단백A/C이구체2등20충차이단백질.결론:다중심발생조여간내전이조다결절간암적단백표체보존재차이.감정출적20개차이표체단백가능삼여간암적발생、발전、침습、전이,유가능성위잠재적、유응용개치적다결절간암세포극륭기원감별분자표지.
Background and Objective:Multinodular hepatocellular carcinoma (HCC) might originate from multicentric occurrence (MO) or intrahepatic metastasis (IM).This study was to find out proteins which play important roles in clonal origin of multinodular hepatocellular carcinoma by screening the differentially expressed proteins between the MO and IM tissues using comparative proteomic analysis.Methods.Total protein extracted from the MO and IM tissues was separated by two-dimensional gel electrophoresis (2-DE).2-DE protein patterns between the MO and IM tissues were compared using computerized image analysis.Proteins exhibiting significant alternations were subsequently isolated and identified by mass spectrometry.Results:A total of (1025±52) and (900±98) spots could be detected in the protein profile in IM and MO tissues,respectively.The expression levels of 25 protein spots were statistically different between the two groups.Twenty of 25 protein spots were identified by MALDI-TOF-MS and bioinformatics.Conclusions:The protein profile of MO HCC tissues was different from that of IM HCC tissues.The twenty differentially expressed proteins might play key roles in the carcinogenesis and progression of multinodular HCC.These newly identified proteins might be potential and valuable biomarkers for identifying the clonal origin of multinodular HCC.
