稀有金属
稀有金屬
희유금속
CHINESE JOURNAL OF RARE METALS
2010年
2期
257-263
,共7页
开链冠醚%稀土配合物%光谱性质%插入作用
開鏈冠醚%稀土配閤物%光譜性質%插入作用
개련관미%희토배합물%광보성질%삽입작용
amide-based open-chain crown ether%rare earth complex%spectrum property%intercalative binding mode
合成了苦味酸铕与N,N.二苄基-N'-苯基-1,1'-联萘-2,2'-二(氧杂乙酰胺)(L)的稀土配合物.经元素分析,IR,TG-DTA和摩尔电导率的表征,确定其组成为Eu(pic)_3L·2H_2O.测定了配合物在不同溶剂中的荧光强度,发现其荧光强度随溶剂配位能力增强而减弱.通过紫外吸收光谱、荧光发射光谱、粘度法,稳态荧光猝灭方法及其与溴化乙啶(EB)的竞争实验研究了配合物与ct-DNA的作用情况,结果显示,配合物与ct-DNA作用时,其紫外吸收产生明显增色效应,荧光强度增强;EB-DNA体系的荧光强度随配合物的加入迅速减弱;在ct-DNA存在下,配合物被猝灭剂K_4[Fe(CN)_6]的发光猝灭程度减小;配合物的加入使ct-DNA的粘度增加;实验结果证明,配合物与ct-DNA以插入方式结合,其结合常数K_q=2.665×10~4L·mol~(-1).
閤成瞭苦味痠銪與N,N.二芐基-N'-苯基-1,1'-聯萘-2,2'-二(氧雜乙酰胺)(L)的稀土配閤物.經元素分析,IR,TG-DTA和摩爾電導率的錶徵,確定其組成為Eu(pic)_3L·2H_2O.測定瞭配閤物在不同溶劑中的熒光彊度,髮現其熒光彊度隨溶劑配位能力增彊而減弱.通過紫外吸收光譜、熒光髮射光譜、粘度法,穩態熒光猝滅方法及其與溴化乙啶(EB)的競爭實驗研究瞭配閤物與ct-DNA的作用情況,結果顯示,配閤物與ct-DNA作用時,其紫外吸收產生明顯增色效應,熒光彊度增彊;EB-DNA體繫的熒光彊度隨配閤物的加入迅速減弱;在ct-DNA存在下,配閤物被猝滅劑K_4[Fe(CN)_6]的髮光猝滅程度減小;配閤物的加入使ct-DNA的粘度增加;實驗結果證明,配閤物與ct-DNA以插入方式結閤,其結閤常數K_q=2.665×10~4L·mol~(-1).
합성료고미산유여N,N.이변기-N'-분기-1,1'-련내-2,2'-이(양잡을선알)(L)적희토배합물.경원소분석,IR,TG-DTA화마이전도솔적표정,학정기조성위Eu(pic)_3L·2H_2O.측정료배합물재불동용제중적형광강도,발현기형광강도수용제배위능력증강이감약.통과자외흡수광보、형광발사광보、점도법,은태형광졸멸방법급기여추화을정(EB)적경쟁실험연구료배합물여ct-DNA적작용정황,결과현시,배합물여ct-DNA작용시,기자외흡수산생명현증색효응,형광강도증강;EB-DNA체계적형광강도수배합물적가입신속감약;재ct-DNA존재하,배합물피졸멸제K_4[Fe(CN)_6]적발광졸멸정도감소;배합물적가입사ct-DNA적점도증가;실험결과증명,배합물여ct-DNA이삽입방식결합,기결합상수K_q=2.665×10~4L·mol~(-1).
A kind of rare earth complex with europium picrate and N, N-dibenzyl-N'-phenyl-1, l'-dinaphthyl-2,2'-Bis ( oxaacet-amide) (L) was synthesized. The complex had the constitution of Eu(pic)3L·2H_20 as characterized by elemental analysis, IR, TG-DTA, and molar conductivity. The fluorescence property of the complex in different solutions was studied. It was found that the fluores-cence intensity of the complex reduced with the raising coordination ability of solvent. DNA-binding properties of the complex were in-vestigated using UV spectra, fluorescent spectra, viscometry, steady-state emission quenching and DNA competitive binding with ethid-ium bromide(EB). The results showed that the intensity of absorption peaks and fluorescence peaks increased when the complex inter-acted with ct-DNA. The complex could make the fluorescence intensity of EB-DNA system decreased sharply; The effect of quencher K_4 [ Fe(CN)_6 ] on the emission of complex-DNA system weakened more than that of the complex. The relative specific viscosity of ct-DNA was increased with the increasing concentration of complex. All the experimental results indicated that the binding-mode between complex and ct-DNA was intercalation. The constant k_q of complex binding to et-DNA was 2.665 × 10~4 L·mol~(-1).
