中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2011年
16期
4-6
,共3页
李乃静%李伟%李岩%李胜岐%何平
李迺靜%李偉%李巖%李勝岐%何平
리내정%리위%리암%리성기%하평
大环内酯类%生物膜%克雷杆菌属%β内酰胺酶类
大環內酯類%生物膜%剋雷桿菌屬%β內酰胺酶類
대배내지류%생물막%극뢰간균속%β내선알매류
Macrolides%Biofilms%Klebsiella%Beta-lactamases
目的 探讨不同浓度阿奇霉素、红霉素、罗红霉素对生物被膜肺炎克雷伯杆菌产β-内酰胺酶的影响.方法 应用改进的平板培养法建立肺炎克雷伯杆菌生物被膜模型,用扫描电镜观察鉴定.取肺炎克雷伯杆菌30株,经37 ℃ 24h孵育,比浊法配制0.5麦氏比浊标准(MCF)菌悬液,分为A组(生物被膜组)、B.组[1最低抑菌浓度(MIC)阿奇霉素生物被膜组]、B2组(1/8 MIC阿奇霉素生物被膜组)、C,组(1 MIC红霉素生物被膜组)、C2组(1/8 MIC红霉素生物被膜组)、D1组(1 MIC罗红霉素生物被膜组)、D2组(1/8 MIC 罗红霉素生物被膜组)七组,每组200μ1.分别检测各组产β-内酰胺酶的活性.结果 A组、B1组、B2组、C1组、C2组、D1组、D2组β-内酰胺酶的活性分别为0.6534、0.3196、0,4260、0.5028、0.5108、0.6524、0.6470 U/mg,B1组、B2组、C1组、C2组β-内酰胺酶活性均明显低于A组,差异有统计学意义(P＜0.05);D1组、D2组β-内酰胺酶活性与A组比较差异无统计学意义(P＞0.05).B1组β-内酰胺酶活性明显低于B2组,差异有统计学意义(P＜0.05);C1组与C2组比较差异无统计学意义(P＞0.05).结论 高浓度的阿奇霉素对生物被膜有较强的破坏作用.
目的 探討不同濃度阿奇黴素、紅黴素、囉紅黴素對生物被膜肺炎剋雷伯桿菌產β-內酰胺酶的影響.方法 應用改進的平闆培養法建立肺炎剋雷伯桿菌生物被膜模型,用掃描電鏡觀察鑒定.取肺炎剋雷伯桿菌30株,經37 ℃ 24h孵育,比濁法配製0.5麥氏比濁標準(MCF)菌懸液,分為A組(生物被膜組)、B.組[1最低抑菌濃度(MIC)阿奇黴素生物被膜組]、B2組(1/8 MIC阿奇黴素生物被膜組)、C,組(1 MIC紅黴素生物被膜組)、C2組(1/8 MIC紅黴素生物被膜組)、D1組(1 MIC囉紅黴素生物被膜組)、D2組(1/8 MIC 囉紅黴素生物被膜組)七組,每組200μ1.分彆檢測各組產β-內酰胺酶的活性.結果 A組、B1組、B2組、C1組、C2組、D1組、D2組β-內酰胺酶的活性分彆為0.6534、0.3196、0,4260、0.5028、0.5108、0.6524、0.6470 U/mg,B1組、B2組、C1組、C2組β-內酰胺酶活性均明顯低于A組,差異有統計學意義(P＜0.05);D1組、D2組β-內酰胺酶活性與A組比較差異無統計學意義(P＞0.05).B1組β-內酰胺酶活性明顯低于B2組,差異有統計學意義(P＜0.05);C1組與C2組比較差異無統計學意義(P＞0.05).結論 高濃度的阿奇黴素對生物被膜有較彊的破壞作用.
목적 탐토불동농도아기매소、홍매소、라홍매소대생물피막폐염극뢰백간균산β-내선알매적영향.방법 응용개진적평판배양법건립폐염극뢰백간균생물피막모형,용소묘전경관찰감정.취폐염극뢰백간균30주,경37 ℃ 24h부육,비탁법배제0.5맥씨비탁표준(MCF)균현액,분위A조(생물피막조)、B.조[1최저억균농도(MIC)아기매소생물피막조]、B2조(1/8 MIC아기매소생물피막조)、C,조(1 MIC홍매소생물피막조)、C2조(1/8 MIC홍매소생물피막조)、D1조(1 MIC라홍매소생물피막조)、D2조(1/8 MIC 라홍매소생물피막조)칠조,매조200μ1.분별검측각조산β-내선알매적활성.결과 A조、B1조、B2조、C1조、C2조、D1조、D2조β-내선알매적활성분별위0.6534、0.3196、0,4260、0.5028、0.5108、0.6524、0.6470 U/mg,B1조、B2조、C1조、C2조β-내선알매활성균명현저우A조,차이유통계학의의(P＜0.05);D1조、D2조β-내선알매활성여A조비교차이무통계학의의(P＞0.05).B1조β-내선알매활성명현저우B2조,차이유통계학의의(P＜0.05);C1조여C2조비교차이무통계학의의(P＞0.05).결론 고농도적아기매소대생물피막유교강적파배작용.
Objective To investigate the effect of azithromycin,erythromycin and roxithromyein at varying concentration on the β-lactamase produced in the biofilm of Klebsiella pneumoniae.Methods Models of Klebsiella pneumoniae were built up with the modified flat-board method and identified with the confocal scanning laser microscopy.Thirty Klebsiella pneumoniae were incubated at 37 ℃for 24 h,and allocated into 7 groups:biofilm (group A),by 1 MIC azithromycin (group B1),by 1/8 MIC azithromycin (group B2),by 1 MIC erythromycin (group C1),by 1/8 MIC erythromycin (group C2),by 1 MIC roxithromycin (group D1),and biofilm induced by 1/8 MIC roxithromycin (group D2),with 200 μ1 each.The β-lactamase activity in each group was quantitated.Results The β-lactamase activity in group A,group B1,group B2,group C1,group C2,group D1 and group D2 was 0.6534,0.3196,0.4260,0.5028,0.5108,0.6524 and 0.6470 U/mg respectively.The β-lactamase activity in group B1,group B2,group C1 and group C2 was lower than that in group A,and statistically significant difference was observed (P＜0.05).The B-lactamase activity in group D1 and group D2 was similar to that in group A,and no statistically significant differences was observed between them (P＞0.05).The β-lactamase activity in group B1 was lower than that in group B2,and statistically significant difference was observed (P <0.05).No statistically significant difference was observed between group C1 and group C2 (P＞0.05).Conclusion Azithromycin at higher concentration exerts better effects on biofilm formation than other macrolides.
