国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2011年
6期
376-379,封3
,共5页
糖尿病%Kir6.2%黄芪多糖%表达
糖尿病%Kir6.2%黃芪多糖%錶達
당뇨병%Kir6.2%황기다당%표체
Diabetes%Kir6.2%Astragalus polysaccharide%Expression
目的 观察不同剂量的黄芪多糖对糖尿病大鼠胰腺Kir6.2表达的干预作用.方法 Wistar大鼠建立糖尿病模型后,随机分为模型组、对照组、低剂量干预组、高剂量干预组,进行糖耐量实验,检测胰腺Kir6.2 mRNA和蛋白表达变化.结果 模型组胰腺Kir6.2表达显著下降,两干预组Kir6.2的mRNA和蛋白表达水平较模型组有不同程度上调(P<0.05).结论 黄芪多糖对糖尿病大鼠胰腺Kir6.2表达有一定的上调作用,可能是其发挥降糖作用的机制之一.
目的 觀察不同劑量的黃芪多糖對糖尿病大鼠胰腺Kir6.2錶達的榦預作用.方法 Wistar大鼠建立糖尿病模型後,隨機分為模型組、對照組、低劑量榦預組、高劑量榦預組,進行糖耐量實驗,檢測胰腺Kir6.2 mRNA和蛋白錶達變化.結果 模型組胰腺Kir6.2錶達顯著下降,兩榦預組Kir6.2的mRNA和蛋白錶達水平較模型組有不同程度上調(P<0.05).結論 黃芪多糖對糖尿病大鼠胰腺Kir6.2錶達有一定的上調作用,可能是其髮揮降糖作用的機製之一.
목적 관찰불동제량적황기다당대당뇨병대서이선Kir6.2표체적간예작용.방법 Wistar대서건립당뇨병모형후,수궤분위모형조、대조조、저제량간예조、고제량간예조,진행당내량실험,검측이선Kir6.2 mRNA화단백표체변화.결과 모형조이선Kir6.2표체현저하강,량간예조Kir6.2적mRNA화단백표체수평교모형조유불동정도상조(P<0.05).결론 황기다당대당뇨병대서이선Kir6.2표체유일정적상조작용,가능시기발휘강당작용적궤제지일.
Objective To investigate the influence of astragalus polysaccharide administration on the the expression of pancreatic Kir6.2 in diabetes rats.Methods Wistar rats were induced diabetes mellitus,then divided into model group,control group,low dose group,and high dose group randomly.After OGTT of them were observed,determination of the Kir6.2 mRNA and protein expression levels in pancreas was performed by RT-PCR and Western blotting.Results The expression of Kir6.2 in model group decreased significantly,and the expressions of Kir6.2 in low dose and hish dose groups were higher than the model group (P<0.05).Conclusion Astragalus polysaccharide displayed up-regulation to the expression of pancreatic Kir6.2.which may be a mechanism on reducing blood glucose.
