CAJ | 학술논문

目的观察大鼠脊髓损伤后继发性损伤中过氧化物酶体增殖物激活受体γ(PPARγ)表达的变化.方法将72只雄性SD大鼠按照随机数字表法分为损伤组(建立大鼠脊髓损伤模型)和对照组(仅显露脊髓,不做打击伤),每组各36只.实时免疫荧光定量多聚酶链反应(FQ-PCR)检测脊髓损伤后1 d、3 d、7 d、14 d、28 d、56 dPPARγmRNA的表达变化,每个时间点取6只大鼠.蛋白印记检测脊髓损伤后1 d、3 d、7 d、14 d、28 d、56 d PPARγ的表达变化.结果与对照组比较,损伤组脊髓组织中PPARγmRNA和PPARγ表达均明显增加,差异均有统计学意义(P＜0.05),其表达高峰在脊髓损伤后14d.结论脊髓损伤后在继发性损伤中PPARγ的表达明显增加,表达高峰在脊髓损伤后14d.
목적 관찰대서척수손상후계발성손상중과양화물매체증식물격활수체γ(PPARγ)표체적변화.방법 장72지웅성SD대서안조수궤수자표법분위손상조(건립대서척수손상모형)화대조조(부현로척수,불주타격상),매조각36지.실시면역형광정량다취매련반응(FQ-PCR)검측척수손상후1 d、3 d、7 d、14 d、28 d、56 dPPARγmRNA적표체변화,매개시간점취6지대서.단백인기검측척수손상후1 d、3 d、7 d、14 d、28 d、56 d PPARγ적표체변화.결과 여대조조비교,손상조척수조직중PPARγmRNA화PPARγ표체균명현증가,차이균유통계학의의(P＜0.05),기표체고봉재척수손상후14d.결론 척수손상후재계발성손상중PPARγ적표체명현증가,표체고봉재척수손상후14d.
Objective To observe the expression changes of peroxisome proliferator activated receptor γ(PPARγ) following the secondary injuries in rats with spinal cord injury (SCI). Methods Seventy-two male SD rats were equally randomized into control group and SCI group. Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed to detect the mRNA expression changes of PPARγ on the 1st, 3nd, 7th 14th, 28th and 56th d of SCI. Western blotting wasemployed to detect the protein expression changes of PPARγ Results Compared with those in the control group, the mRNA and protein expressions of PPARγ in the SCI group on the 1st, 3rd, 7th, 14th and 28th d of SCI were significantly increased (P＜0.05), reaching its peak level 14 d after SCI; on the 56th d of SCI, their expression levels in the SCI group were still higher than those in the control group, but no significant differences were noted (P＞0.05). Conclusion The expressions of PPARγare significantly increased following secondary injuries in rats with spinal cord injury, reaching its peak level 14 d after SCI.