CAJ | 학술논문

目的测定帕金森病(PD)患者尿中8-异前列腺素F2α(8-iso-PGF2α)和血中低密度脂蛋白体外氧化延迟时间的变化. 方法运用病例一对照观察,根据Caine标准选取31例男性PD患者,同时选取31例年龄、影响因素等相当的健康男性为对照.通过铜氧化共轭双烯法在波长234nm测定血中低密度脂蛋白体外氧化延迟时间以及酶联免疫法测定尿中8-iso-PGF2α浓度. 结果 PD患者组尿中8-iso-PGF2α含量比对照组明显增高[(81.1±1.6)ng/mmol肌酐vs(46.9±1.1)ng/mmol肌酐],差异有统计学意义(P<0.05);而血中低密度脂蛋白体外氧化延迟时间明显减低[(63.5±6.0)minvs(84.4±8.8)min],差异有统计学意义(P<0.05). 结论增高的氧化压力与降低的抗氧化能力在PD的病理过程中具有一定的作用.提示有效的抗氧化治疗可能对控制疾病的发展具有一定的意义.
목적 측정파금삼병(PD)환자뇨중8-이전렬선소F2α(8-iso-PGF2α)화혈중저밀도지단백체외양화연지시간적변화. 방법 운용병례일대조관찰,근거Caine표준선취31례남성PD환자,동시선취31례년령、영향인소등상당적건강남성위대조.통과동양화공액쌍희법재파장234nm측정혈중저밀도지단백체외양화연지시간이급매련면역법측정뇨중8-iso-PGF2α농도. 결과 PD환자조뇨중8-iso-PGF2α함량비대조조명현증고[(81.1±1.6)ng/mmol기항vs(46.9±1.1)ng/mmol기항],차이유통계학의의(P<0.05);이혈중저밀도지단백체외양화연지시간명현감저[(63.5±6.0)minvs(84.4±8.8)min],차이유통계학의의(P<0.05). 결론 증고적양화압력여강저적항양화능력재PD적병리과정중구유일정적작용.제시유효적항양화치료가능대공제질병적발전구유일정적의의.
Objective To measure the changes in urinary 8-iso-prostaglandin-F2α (8-iso-PGF2α) and oxidized low-density lipoprotein (ox-LDL) lag time in patients with Parkinson's disease (PD). Methods A case-control study was performed involving 31 male patients with PD (mean age of 59.7 years) diagnosed according to the Calne criteria and 31 age-matched healthy male subjects with comparable status of smoking and life style. For each subject, urinary 8-iso-PGF2α was measured quantitatively using enzyme-linked immunosorbent assay, and LDL oxidizability was measured by determining LDL oxidation lag time in conjugated diene product at 234 nm using Cu-stimulated oxidation. Results Compared to the levels in the control subjects, the PD patients showed significantly increased urinary 8-iso-PGF2α (81.1±1.6 vs 46.9±1.1 ng/mmol creatinine, P<0.05) and significantly reduced LDL oxidation lag time (63.5±6.0 vs 84.4±8.8 min, P<0.05). Conclusion Increased 8-iso-PGF2α and decreased anti-oxidant ability are implicated in the pathogenesis of PD, suggesting the value of appropriate antioxidant therapy in controlling the progression of PD.