CAJ | 학술논문

背景:冠脉支架后较高的早期急性再闭塞及晚期再狭窄已经成为研究的热点,而生物降解材料携带对抑制再狭窄有效的药物-紫杉醇金属涂层支架有望解决这一问题.目的:寻找出一种用于制作生物可降解支架的生物可降解材料.设计、时间及地点:对比观察的细胞学实验,于2003-07/2005-07在吉林大学公共卫生学院毒理实验室(卫生部放射牛物学重点实验室、吉林省毒理学重点实验室)完成.材料:紫杉醇可降解材料(左旋丙素酯与聚乙交酯的比例为9:1)由中国科学院长春应用化学研究所提供.人脐动脉平滑肌细胞为武汉博士德生物有限公司产品.方法:将培养所得第6代平滑肌细胞用0.25%胰蛋白酶在室温下消化3 min,加入10%牛血清BMEM培养液10 mL,制成平滑肌细胞悬液.将表面处理后用60Co消毒的低温保存材料经水浴加热至37℃,取出置于6孔培养板中,将细胞悬液滴加至材料中部,植入细胞浓度约5×106/cm2,使细胞悬液缓慢向材料周围扩散,最后加入10%BMEM牛血清培养液,放入5%CO2孵箱37℃中培养,每天用倒置显微镜观察材料中及周边细胞生长状态.主要观察指标:分别于培养后第3,6,12,19,26,34天后将材料取出,真空干燥、称质量,比较培养前后材料重量的损失,并计算材料平均降解速率.结果:倒置显微镜观察降解材料对人脐动脉平滑肌细胞的生长没有影响,体外培养的平均降解速率为0.45%/d.结论:此紫杉醇降解材料具有良好的细胞相容性,符合制作血管内支架材料的要求.
배경:관맥지가후교고적조기급성재폐새급만기재협착이경성위연구적열점,이생물강해재료휴대대억제재협착유효적약물-자삼순금속도층지가유망해결저일문제.목적:심조출일충용우제작생물가강해지가적생물가강해재료.설계、시간급지점:대비관찰적세포학실험,우2003-07/2005-07재길림대학공공위생학원독리실험실(위생부방사우물학중점실험실、길림성독이학중점실험실)완성.재료:자삼순가강해재료(좌선병소지여취을교지적비례위9:1)유중국과학원장춘응용화학연구소제공.인제동맥평활기세포위무한박사덕생물유한공사산품.방법:장배양소득제6대평활기세포용0.25%이단백매재실온하소화3 min,가입10%우혈청BMEM배양액10 mL,제성평활기세포현액.장표면처리후용60Co소독적저온보존재료경수욕가열지37℃,취출치우6공배양판중,장세포현액적가지재료중부,식입세포농도약5×106/cm2,사세포현액완만향재료주위확산,최후가입10%BMEM우혈청배양액,방입5%CO2부상37℃중배양,매천용도치현미경관찰재료중급주변세포생장상태.주요관찰지표:분별우배양후제3,6,12,19,26,34천후장재료취출,진공간조、칭질량,비교배양전후재료중량적손실,병계산재료평균강해속솔.결과:도치현미경관찰강해재료대인제동맥평활기세포적생장몰유영향,체외배양적평균강해속솔위0.45%/d.결론:차자삼순강해재료구유량호적세포상용성,부합제작혈관내지가재료적요구.
BACKGROUND: The problem of the high rate of acute restenosis at an early and advanced stage has been a hot spot, while the stent with paclitaxel (poly-L-lactide, PLLA/polyglycolic acid, PGA) degradable material will resolve it. OBJECTIVE: To seek an ideal biologic degradable material used in biologic degradable stent. DESIGN, TIME AND SETTING: The comparative cytological study was performed at the Laboratory of Toxicology, Institute of Public Health, Jilin University (Key Laboratory of Radiobiology of Ministry of Public Health of China, Key Laboratory of Toxicology of Jilin Province) from July 2003 to July 2005. MATERIALS: Paclitaxel degradable material (PLLA/PGA) (PLLA:PGA= 9: l ) were offered by Changchun Applied Chemistry Institute of Chinese Academy of Science). Human umbilical arterial smooth muscle cells (SMCs) were purchased from Boster, Wuhan, China. METHODS: The sixth passage of SMCs were digested by 0.25% Trypsin under ambient temperature for 3 minutes, incubated in 10 mL 10% ox serum BMEM, and made into SMC suspension. The materials kept in hypothermal disinfectant Co60 were heated to 37 ℃ by waterbath after surface treatment, and then placed in a 6-well culture plate. SMC suspension was added into the middle of the materials. The density of cells was about 5×106/cm2. Cell suspension diffused around the materials gradually. At last, 10% ox serum BMEM culture solution was added into it, and cultivated in a 5% CO2 incubator at 37 ℃. The growth of cells in and surrounding the materials was observed by inverted microscope everyday. MAIN OUCOME MEASURES: The materials were obtained after culture for 3, 6, 12, 19, 26, 34 days and vacuum dehydration, and were weighed. The weight loss of materials was compared before and after culture. The average degradable rate of materials was calculated. RESULTS: Degradable material had no influence on the development of SMCs. The average degradation rate ex vivo was 0.45% per day. CONCLUSION: PLLA/PGA with good cellular compatibility could be applied to intravascular stents.