CAJ | 학술논문

以6个桃品种为研究对象,采用RAPD技术对其进行鉴定和亲缘关系分析.25个随机引物共获得213条DNA谱带,其中65条(30.5%)为多态性带.通过单引物和引物组合可以将6个品种区分开来.RAPD分析结果表明;"秋红晚蜜"和"秋红早蜜"先聚在-起,然后再与"秋红蜜"聚在-起,三者间遗传相似系数为0.983～0.984,为共同起源的地方性品种;"燕红"和"西妃"先聚在-起,然后又与"乐林早红"聚在-起,三者相似系数范围为0.913～0.980,其中`燕红"和"西妃"的相似系数为0.980.推测"西妃"是"燕红"的芽变或者亲本来源相同.
이6개도품충위연구대상,채용RAPD기술대기진행감정화친연관계분석.25개수궤인물공획득213조DNA보대,기중65조(30.5%)위다태성대.통과단인물화인물조합가이장6개품충구분개래.RAPD분석결과표명;"추홍만밀"화"추홍조밀"선취재-기,연후재여"추홍밀"취재-기,삼자간유전상사계수위0.983～0.984,위공동기원적지방성품충;"연홍"화"서비"선취재-기,연후우여"악림조홍"취재-기,삼자상사계수범위위0.913～0.980,기중`연홍"화"서비"적상사계수위0.980.추측"서비"시"연홍"적아변혹자친본래원상동.
In this study,6 peach cuhivars were analyzed using random amplified polymorphicDNA markers.213 DNA bands were amplified with 25 primers,65 of which(30.5%)Werepolymorphic.Six cuhivars can be differentiated through single primer and double primers.Theresult of cluster analysis showed that'Qiuhongwanmi'and'Qiuhongzaomi'were clustered 'first,and then clustered with'Qiuhongmi'.and the genetic similarity coefficients of the threecuhivars ranged from 0.983 to 0.984;They were local cuhivars originated from the same culti-var.'Yanhong'and'Xifei'were clustered first,and then clustered with'Laolinzaohong'.The range of genetic similarity coefficients of the three cuhivars was from 0.913 to 0.980,'Yanhong'and'Xifei'of which were 0.980.'Xifei'maybe sport from'Yanhong'or origina-ted from the same parents presumably.