CAJ | 학술논문

目的研究溶胶-凝胶生物活性玻璃/胶原组织工程支架的力学性能及降解性能,为胶原基复合支架的进一步应用,提供理论基础.方法以纳米溶胶.凝胶生物活性玻璃为添加相,利用冷冻干燥法制备了4种溶胶-凝胶生物活性玻璃/胶原基复合多孔组织工程支架.结果 (1)溶液中胶原纤维的聚集状态,制备出具有直径约为400～600 nm的粗胶原纤维束支架材料,这种粗胶原纤维束对改善胶原基组织工程支架的力学强度和减慢其降解速度具有重要作用,其中胶原与生物活性玻璃质量比为40:60时,具有最高的抗压强度(1.5469±0.0995)MPa.(2)利用FTIR和Raman等技术综合分析研究了溶胶-凝胶生物活性玻璃对胶原蛋白的二级结构的影响,当复合材料中胶原含鼍小于20%时,胶原蛋白二级结构破坏严重.结论当胶原与生物活性玻璃质量比为40:60时,所制备的复合支架具有最好的抗压性能和降解性能,为进一步应用提供了研究基础.
목적 연구용효-응효생물활성파리/효원조직공정지가적역학성능급강해성능,위효원기복합지가적진일보응용,제공이론기출.방법 이납미용효.응효생물활성파리위첨가상,이용냉동간조법제비료4충용효-응효생물활성파리/효원기복합다공조직공정지가.결과 (1)용액중효원섬유적취집상태,제비출구유직경약위400～600 nm적조효원섬유속지가재료,저충조효원섬유속대개선효원기조직공정지가적역학강도화감만기강해속도구유중요작용,기중효원여생물활성파리질량비위40:60시,구유최고적항압강도(1.5469±0.0995)MPa.(2)이용FTIR화Raman등기술종합분석연구료용효-응효생물활성파리대효원단백적이급결구적영향,당복합재료중효원함타소우20%시,효원단백이급결구파배엄중.결론 당효원여생물활성파리질량비위40:60시,소제비적복합지가구유최호적항압성능화강해성능,위진일보응용제공료연구기출.
Objective To investigate the mechanical prosperity and degradation rate of the scaffolds with compounding collagen and the nano sol-gel derived bioactive glass were studied,and provide the theoretical basis for the further application of collagen based scaffolds.Method The scaffold with compounding collagen and the nano sol-gel derived bioactive glass(58S)were prepared using the freeze-drying techniques with the bioactive glass as phase addition.By affecting the aggregation state of the collagen fibers with adjusting the supplementation of bioactive glass to change the microstructures of the compound scaffolds and finatly the compound scaffolds with different mechanical properties were prepared.Results (1)As the aggregation state of the collagen fibers changes,the scaffolds with the coarser collagen fibers is prepared with the diameters 400-600 nm approximately.The coarser collagen fibers will play an important role in improving the mechanical property and slowing down the degradation rate of the collagen based scaffolds.(2)The interactions between bioactive glass and collagen are studied by FTIR and Raman technologies.When the quality of content of collagen in the compound scaffolds is lower than 20%,the secondary structure of collagen is damaged severely.Conclusion The composite scaffolds with the mass ratio of collagen to bioactive glass 40:60 has the best performance in mechanical property and degradation,which will be helpful for further applications.