中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
3期
318-321
,共4页
吕宝勇%闫军%李宁%张叶峰
呂寶勇%閆軍%李寧%張葉峰
려보용%염군%리저%장협봉
急性胰腺炎%精氨酸%瘦素%核因子-κB%肿瘤坏死因子-α
急性胰腺炎%精氨痠%瘦素%覈因子-κB%腫瘤壞死因子-α
급성이선염%정안산%수소%핵인자-κB%종류배사인자-α
Acute pancreatitis%Arginine%Leptin%Nuclear factor-κB%Tumor necrosis factor-α
目的 探讨精氨酸联合瘦素治疗大鼠急性胰腺炎的疗效.方法 Wister大鼠60只,随机分为假手术组(A组)、急性胰腺炎组(B组)、精氨酸治疗组(C组)、瘦素治疗组(D组)和联合治疗组(E组),每组12只;采用5%牛磺胆酸钠胰胆管逆行注入制作急性胰腺炎模型.每组大鼠均在腹腔注射治疗6 h后行腹主动脉采血、取胰腺标本;检测每组血清肿瘤坏死因子-α(TNF-α)的含量、淀粉酶;胰腺组织核因子-κB(NF-κB)活性,并对胰腺损伤进行病理评分.结果 A组TNF-α的含量、淀粉酶、NF-κB活性、病理评分依次为(1.206±0.446)μg/L、(1617.2±18.8)U/L、0.174±0.013、0.6±0.2;B组依次为(3.201±0.321)μg/L、(4643.8±303.4)U/L、0.397±0.025、7.8±1.3;C组依次为(1.845±0.404)μg/L、(3370.9±173.2)U/L、0.255±0.026、3.9±1.1;D组依次为(1.996±0.374)μg/L、(3693.7±208.0)U/L、0.274±0.062、4.2±1.2;E组依次为(1.440±0.287)μg/L、(2501.0±771.4)U/L、0.211±0.027、2.3±1.0.与A组相比,B组上述指标均增高明显(P均<0.05);与B组相比,C组和D组以上指标均降低(P均<0.05),而C组与D组间差异均无统计学意义(P均>0.05);E组较C组和D组的上述指标均明显降低(P均<0.05).且NF-κB与其余指标均呈正相关(P<0.01).结论 精氨酸和瘦素在治疗大鼠急性胰腺炎过程中疗效确切且两药联合治疗效果优于单一用药.
目的 探討精氨痠聯閤瘦素治療大鼠急性胰腺炎的療效.方法 Wister大鼠60隻,隨機分為假手術組(A組)、急性胰腺炎組(B組)、精氨痠治療組(C組)、瘦素治療組(D組)和聯閤治療組(E組),每組12隻;採用5%牛磺膽痠鈉胰膽管逆行註入製作急性胰腺炎模型.每組大鼠均在腹腔註射治療6 h後行腹主動脈採血、取胰腺標本;檢測每組血清腫瘤壞死因子-α(TNF-α)的含量、澱粉酶;胰腺組織覈因子-κB(NF-κB)活性,併對胰腺損傷進行病理評分.結果 A組TNF-α的含量、澱粉酶、NF-κB活性、病理評分依次為(1.206±0.446)μg/L、(1617.2±18.8)U/L、0.174±0.013、0.6±0.2;B組依次為(3.201±0.321)μg/L、(4643.8±303.4)U/L、0.397±0.025、7.8±1.3;C組依次為(1.845±0.404)μg/L、(3370.9±173.2)U/L、0.255±0.026、3.9±1.1;D組依次為(1.996±0.374)μg/L、(3693.7±208.0)U/L、0.274±0.062、4.2±1.2;E組依次為(1.440±0.287)μg/L、(2501.0±771.4)U/L、0.211±0.027、2.3±1.0.與A組相比,B組上述指標均增高明顯(P均<0.05);與B組相比,C組和D組以上指標均降低(P均<0.05),而C組與D組間差異均無統計學意義(P均>0.05);E組較C組和D組的上述指標均明顯降低(P均<0.05).且NF-κB與其餘指標均呈正相關(P<0.01).結論 精氨痠和瘦素在治療大鼠急性胰腺炎過程中療效確切且兩藥聯閤治療效果優于單一用藥.
목적 탐토정안산연합수소치료대서급성이선염적료효.방법 Wister대서60지,수궤분위가수술조(A조)、급성이선염조(B조)、정안산치료조(C조)、수소치료조(D조)화연합치료조(E조),매조12지;채용5%우광담산납이담관역행주입제작급성이선염모형.매조대서균재복강주사치료6 h후행복주동맥채혈、취이선표본;검측매조혈청종류배사인자-α(TNF-α)적함량、정분매;이선조직핵인자-κB(NF-κB)활성,병대이선손상진행병리평분.결과 A조TNF-α적함량、정분매、NF-κB활성、병리평분의차위(1.206±0.446)μg/L、(1617.2±18.8)U/L、0.174±0.013、0.6±0.2;B조의차위(3.201±0.321)μg/L、(4643.8±303.4)U/L、0.397±0.025、7.8±1.3;C조의차위(1.845±0.404)μg/L、(3370.9±173.2)U/L、0.255±0.026、3.9±1.1;D조의차위(1.996±0.374)μg/L、(3693.7±208.0)U/L、0.274±0.062、4.2±1.2;E조의차위(1.440±0.287)μg/L、(2501.0±771.4)U/L、0.211±0.027、2.3±1.0.여A조상비,B조상술지표균증고명현(P균<0.05);여B조상비,C조화D조이상지표균강저(P균<0.05),이C조여D조간차이균무통계학의의(P균>0.05);E조교C조화D조적상술지표균명현강저(P균<0.05).차NF-κB여기여지표균정정상관(P<0.01).결론 정안산화수소재치료대서급성이선염과정중료효학절차량약연합치료효과우우단일용약.
Objective To investigate the effects of arginine combined with leptin in treatment of rats with a-cute pancreatitis. Methods Sixty Wister rats were randomly divided into sham operation group (group A), acute pancreatitis group (group B),arginine group (group C),leptin group (group D),arginine plus leptin group (group E),with 12 rats in each group. Acute panereatitis model was developed by retrograde injection of 5% sodium tauro-cholate into cholangiopancreatography in rats. After 6 hours, medicine were given by intraperitoneal injection, ab-dominal aortic blood and pancreatic specimens were taken for measurement of tumor necrosis factor-α(TNF-α), ser-um amylase,nuclear factor-κB (NF-κB) activity of pancreatic tissue, and pathological damage score of pancreatic tissue. Results TNF-α,Amylase,NF-κB activity, pathologic scores were(1.206±0.446)μg/L, (1617.2±18.8) U/L,0.174±0.013,0.6±0. 2 in group A;(3.201±0.321) μg/L, (4643.8±303.4) U/L,0.397±0. 025,7.8±1.3 in group B;(1.845±0.404) μg/L,(3370.9±173.2)U/L,0.255±0.026,3.9±1.1 in group C;(1.996±0.374) μg/L, (3693.7±208.0)U/L,0.274±0.062,4.2±1.2 in group D;(1.440±0.287)μg/L, (2501.0±771.4)U/L,0.211±0.027,2.3±1.0 in group E. Compared with group A, the indicators were significantly in-creased in group B (P < 0.05). Compared with group B, these indicators were reduced in group C and D (P <0.05). There was no significant difference between group C and group D. These indicators in group E were signifi-cantly lower than those in group C and D (P < 0.05). NF-κB in pancreatic tissue were positively correlated with other indicators(P <0.01). Conclusions In treatment of acme pancreatitis in rats, arginine combined with leptin has idenditfied effect, and the effect of combination therapy is better than that of separate medicine.
