中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2008年
6期
447-450
,共4页
李玉晓%陈冬%李添应%冯炼强%许庚%文卫平
李玉曉%陳鼕%李添應%馮煉彊%許庚%文衛平
리옥효%진동%리첨응%풍련강%허경%문위평
模型,动物%鼻炎,变应性,季节性%大蒜辣素%白细胞介素4%干扰素Ⅱ型
模型,動物%鼻炎,變應性,季節性%大蒜辣素%白細胞介素4%榦擾素Ⅱ型
모형,동물%비염,변응성,계절성%대산랄소%백세포개소4%간우소Ⅱ형
Models,animal%Rhinitis,allergic,seasonal%Alliein%Interleukin-4%Interferon type Ⅱ
目的 探讨大蒜新素对大鼠变应性鼻炎模型外周血中愀扇潘和白细胞介素4(IL-4)的影响.方法 健康雌性SD大鼠30只,随机分为对照组、空白组和实验组,每组10只.实验组用卵清蛋白辅以氢氧化铝和灭活百日咳杆菌佐剂建立变应性鼻炎模型,成功后连续腹腔注射大蒜新素10 d;对照组造模方法同实验组,成功后腹腔注射等体积的生理盐水;空白组除无卵清蛋白致敏和激发外,方法同对照组.颈内静脉取血,分离血浆,采用酶联免疫吸附试验(ELISA)检测血浆中IL-4和INF-愕暮俊＝峁实验组、对照组和空白组大鼠外周血浆中IL-4水平(-x±s,下同)分别为(22.81±8.79)pg/L、(41.43±4.93)pg/L和(9.93±2.07)pg/L;INF-愕乃椒直鹗.32±11.20)pg/L,(11.35±2.45)pg/L和(21.69±5.93)pg/L.实验组血浆中IL-4的水平低于对照组而高于空白组,差异具有统计学意义(t值分别为3.22及4.17,P值均<0.05);实验组血浆中INF-γ水平高于对照组(t=3.84,P<0.05),而与空白组相比差异无统计学意义(t=1.47,P>0.05).结论 大蒜新素能降低大鼠变应性鼻炎模型外周血中IL-4的水平,同时提高INF-γ的水平,对Th1和Th2细胞因子水平具有调节作用.
目的 探討大蒜新素對大鼠變應性鼻炎模型外週血中愀扇潘和白細胞介素4(IL-4)的影響.方法 健康雌性SD大鼠30隻,隨機分為對照組、空白組和實驗組,每組10隻.實驗組用卵清蛋白輔以氫氧化鋁和滅活百日咳桿菌佐劑建立變應性鼻炎模型,成功後連續腹腔註射大蒜新素10 d;對照組造模方法同實驗組,成功後腹腔註射等體積的生理鹽水;空白組除無卵清蛋白緻敏和激髮外,方法同對照組.頸內靜脈取血,分離血漿,採用酶聯免疫吸附試驗(ELISA)檢測血漿中IL-4和INF-愕暮俊＝峁實驗組、對照組和空白組大鼠外週血漿中IL-4水平(-x±s,下同)分彆為(22.81±8.79)pg/L、(41.43±4.93)pg/L和(9.93±2.07)pg/L;INF-愕迺椒直鶚.32±11.20)pg/L,(11.35±2.45)pg/L和(21.69±5.93)pg/L.實驗組血漿中IL-4的水平低于對照組而高于空白組,差異具有統計學意義(t值分彆為3.22及4.17,P值均<0.05);實驗組血漿中INF-γ水平高于對照組(t=3.84,P<0.05),而與空白組相比差異無統計學意義(t=1.47,P>0.05).結論 大蒜新素能降低大鼠變應性鼻炎模型外週血中IL-4的水平,同時提高INF-γ的水平,對Th1和Th2細胞因子水平具有調節作用.
목적 탐토대산신소대대서변응성비염모형외주혈중초선반화백세포개소4(IL-4)적영향.방법 건강자성SD대서30지,수궤분위대조조、공백조화실험조,매조10지.실험조용란청단백보이경양화려화멸활백일해간균좌제건립변응성비염모형,성공후련속복강주사대산신소10 d;대조조조모방법동실험조,성공후복강주사등체적적생리염수;공백조제무란청단백치민화격발외,방법동대조조.경내정맥취혈,분리혈장,채용매련면역흡부시험(ELISA)검측혈장중IL-4화INF-악모준＝묘실험조、대조조화공백조대서외주혈장중IL-4수평(-x±s,하동)분별위(22.81±8.79)pg/L、(41.43±4.93)pg/L화(9.93±2.07)pg/L;INF-악내초직악.32±11.20)pg/L,(11.35±2.45)pg/L화(21.69±5.93)pg/L.실험조혈장중IL-4적수평저우대조조이고우공백조,차이구유통계학의의(t치분별위3.22급4.17,P치균<0.05);실험조혈장중INF-γ수평고우대조조(t=3.84,P<0.05),이여공백조상비차이무통계학의의(t=1.47,P>0.05).결론 대산신소능강저대서변응성비염모형외주혈중IL-4적수평,동시제고INF-γ적수평,대Th1화Th2세포인자수평구유조절작용.
Objective To investigate the effect of garlicin on the levels of interferon interleukin 4(IL-4)in blood of alhrgic rhinitis rat model Methods Thirty healthy female SD rats were randomly divided into 3 groups:control group,negative control group and experimental group,10 rats for each group.Ten rata(experimental group)were sensitized and intranasally challenged by ovalbumin,aluminium hyrdroxide hydrate gel and Bordetella pertussis inactive microorganism suspension adjuvants,as allergic rhinitis models.and then injection of garliein(0.4 ml)intraperitoneally per day for 10 days.Control group rats were immunized as experimental group,and then injection of physiological saline 8.8 equal volume as garlicin.Negative control group rata were investigated using physiological saline.Blood of intrajugular vein of rat was extracted for separated plasma.Enzyme liked immunosorbent assay(ELISA)was utilized to pg/L,(41.43±4.93)pg/L,(9.93±2.07)pg/L,and those of IFN-.32±11.20)pg/L,(11.35±2.45)pg/L and(21.69±5.93)pg/L,respectively,among experimental group,control group and negative control group.The senun level of IL-4 in experimental group rata was lower than value of control group rats(t=3.22,P<0.05),while higher than negative control group(t=4.17,P<0.05).rI'lle serum experimental group and negative control group(t:1.47,P>0.05).Conclusions Garlicin could increase selq.1m level of INF- rat modeL It played an important role on regulating serum levels of cytokines of Thl and Th2.
