中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2009年
4期
207-210
,共4页
汪亮%沈彤%周承藩%于均峰%朱启星
汪亮%瀋彤%週承藩%于均峰%硃啟星
왕량%침동%주승번%우균봉%주계성
三氯乙烯%皮肤%一氧化氮%维生素E
三氯乙烯%皮膚%一氧化氮%維生素E
삼록을희%피부%일양화담%유생소E
Trichloroethylene%Skin%Nitrogen monoxidum%Vitamin E
目的 研究三氯乙烯(TCE)刺激对BALB/c裸鼠皮肤一氧化氮(NO)含量的影响,并观察银杏叶提取物(GbE)和维生素E(VE)的保护作用.方法 将132只BALB/c裸鼠随机分成空白对照组、溶剂对照组(橄榄油)、TCE组(20%TCE、40%TCE、80%TCE和100%TCE)、GbE保护组(0.1%GbE、1%GbE和10%GbE)和VE保护组(5%VE、10%VE和20%VE),每组11只,其中5只用于急性刺激试验,6只用于累积性刺激试验,观察皮肤刺激反应,并检测背部皮肤组织中NO的含量.结果 (1)各浓度TCE处理后裸鼠背部皮肤均产生不同程度的红斑和水肿,随着TCE浓度的升高以及染毒时间延长,皮肤的炎症反应程度逐渐加重.10%GbE组和20%VE组皮肤基本保持完好状态.(2)在急性刺激试验中,80%、100%TCE组NO含量分别为(69.895±9.605)和(77.273±9.290)μmol/mg蛋白.与空白对照组和溶剂对照组比较,差异有统计学意义(P<0.05或P<0.01);各浓度GbE和VE预处理均能降低NO含量.与100%TCE组比较.差异均有统计学意义(P<0.05或P<0.01).(3)在累积性刺激试验中,80%、100%TCE组NO含量分别为(60.362±9.817)和(68.027±9.354)μmol/mg蛋白,与空白对照和溶剂对照组相比,差异均有统计学意义(P<0.05或P<0.01);1%GbE和10%GbE组以及10%VE和20%VE组NO含量明显降低,与100%TCE组比较.差异有统计学意义(P<0.05或P<0.01).结论 TCE对BALB/c裸鼠皮肤产生刺激作用,并诱导皮肤中NO含量升高;GbE和VE对TCE所引起的皮肤损伤有一定的保护作用.
目的 研究三氯乙烯(TCE)刺激對BALB/c裸鼠皮膚一氧化氮(NO)含量的影響,併觀察銀杏葉提取物(GbE)和維生素E(VE)的保護作用.方法 將132隻BALB/c裸鼠隨機分成空白對照組、溶劑對照組(橄欖油)、TCE組(20%TCE、40%TCE、80%TCE和100%TCE)、GbE保護組(0.1%GbE、1%GbE和10%GbE)和VE保護組(5%VE、10%VE和20%VE),每組11隻,其中5隻用于急性刺激試驗,6隻用于纍積性刺激試驗,觀察皮膚刺激反應,併檢測揹部皮膚組織中NO的含量.結果 (1)各濃度TCE處理後裸鼠揹部皮膚均產生不同程度的紅斑和水腫,隨著TCE濃度的升高以及染毒時間延長,皮膚的炎癥反應程度逐漸加重.10%GbE組和20%VE組皮膚基本保持完好狀態.(2)在急性刺激試驗中,80%、100%TCE組NO含量分彆為(69.895±9.605)和(77.273±9.290)μmol/mg蛋白.與空白對照組和溶劑對照組比較,差異有統計學意義(P<0.05或P<0.01);各濃度GbE和VE預處理均能降低NO含量.與100%TCE組比較.差異均有統計學意義(P<0.05或P<0.01).(3)在纍積性刺激試驗中,80%、100%TCE組NO含量分彆為(60.362±9.817)和(68.027±9.354)μmol/mg蛋白,與空白對照和溶劑對照組相比,差異均有統計學意義(P<0.05或P<0.01);1%GbE和10%GbE組以及10%VE和20%VE組NO含量明顯降低,與100%TCE組比較.差異有統計學意義(P<0.05或P<0.01).結論 TCE對BALB/c裸鼠皮膚產生刺激作用,併誘導皮膚中NO含量升高;GbE和VE對TCE所引起的皮膚損傷有一定的保護作用.
목적 연구삼록을희(TCE)자격대BALB/c라서피부일양화담(NO)함량적영향,병관찰은행협제취물(GbE)화유생소E(VE)적보호작용.방법 장132지BALB/c라서수궤분성공백대조조、용제대조조(감람유)、TCE조(20%TCE、40%TCE、80%TCE화100%TCE)、GbE보호조(0.1%GbE、1%GbE화10%GbE)화VE보호조(5%VE、10%VE화20%VE),매조11지,기중5지용우급성자격시험,6지용우루적성자격시험,관찰피부자격반응,병검측배부피부조직중NO적함량.결과 (1)각농도TCE처리후라서배부피부균산생불동정도적홍반화수종,수착TCE농도적승고이급염독시간연장,피부적염증반응정도축점가중.10%GbE조화20%VE조피부기본보지완호상태.(2)재급성자격시험중,80%、100%TCE조NO함량분별위(69.895±9.605)화(77.273±9.290)μmol/mg단백.여공백대조조화용제대조조비교,차이유통계학의의(P<0.05혹P<0.01);각농도GbE화VE예처리균능강저NO함량.여100%TCE조비교.차이균유통계학의의(P<0.05혹P<0.01).(3)재루적성자격시험중,80%、100%TCE조NO함량분별위(60.362±9.817)화(68.027±9.354)μmol/mg단백,여공백대조화용제대조조상비,차이균유통계학의의(P<0.05혹P<0.01);1%GbE화10%GbE조이급10%VE화20%VE조NO함량명현강저,여100%TCE조비교.차이유통계학의의(P<0.05혹P<0.01).결론 TCE대BALB/c라서피부산생자격작용,병유도피부중NO함량승고;GbE화VE대TCE소인기적피부손상유일정적보호작용.
Objective To study the changes of nitric oxide (NO) in the BALB/c hairless mice skin after trichloroethylene (TCE) irritation and the protection of ginkgo biloba extract(GbE) and vitamin E (VE).Methods 132 BALB/c hairless mice were randomly divided into blank control group, solvent group (olive oil) , TCE groups(20%TCE, 40%TCE, 80%TCE and 100%TCE),GbE groups(0.1%GbE,1%GbE and 10% GbE) and VE groups (5%VE,10% VE and 20% VE),with 11 animals in each group, 5 for acute irritation test and 6 for the cumulative irritation test. The skin irritation was observed, and the levels of NO in the dorsal skin of BALB/C hairless mice were detected. The kit of NO was used to detect the levels of NO in the dorsal skin of BALB/c hairless mice. Results (1) The skin presented erythema and edema after TICE ir-ritation both in acute irritation and cumulative irritation test and the skin inflammation showed time-dose ef-fect relationship; the mice skin was protected in GbE or VE groups. (2) In the acute stimulation test, the levels of NO in 80%TCE group (69.895±9.605 μmol/mg pro) and 100%TCE group (77.273±9.290 μmol/mg pro) were significantly different compared with blank control group and solvent control group (P<0.05 or P<0.01). In the protection group, the NO level were reduced, with the statistically significant differences. (3) In acute irritation test, the levels of NO in 80%TCE group (60.362±9.817 μmol/mg pro) and 100%TCE group (68.027±9.354 μmol/mg pro) were significantly different compared with blank control group and solvent control group,(P<0.05 or P<0.01); In the protection group, 1% GbE ,10% GbE ,10% VE and 20%VE could re-duce the levels of NO, with statistically significant differences. Conclusion TCE can produce the irritation on the dorsal skin of BALB/c hairless mice and induce the significant increase of the NO levels. GbE and VE can protect the skin from TCE irritation damage.
