中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
7期
492-495
,共4页
李宜儒%姚卫锋%盛彩虹%冯斌%展小飞%李玲杰%尤聪%李艳飞%刘原君%齐蔓莉%刘全忠
李宜儒%姚衛鋒%盛綵虹%馮斌%展小飛%李玲傑%尤聰%李豔飛%劉原君%齊蔓莉%劉全忠
리의유%요위봉%성채홍%풍빈%전소비%리령걸%우총%리염비%류원군%제만리%류전충
衣原体,沙眼%细菌外膜蛋白质类%疫苗%动物实验
衣原體,沙眼%細菌外膜蛋白質類%疫苗%動物實驗
의원체,사안%세균외막단백질류%역묘%동물실험
Chlamydia trachomatis Bacterial outer membrane proteins%Vaccines%Animal experimentation
[目的]探讨E型重组主外膜蛋白(rMOMP)对恒河猴诱导产生的特异性免疫应答效应.[方法]恒河猴分3组,每组2只,分别为佐剂蛋白组、佐剂组、对照组.各组均于第0(免疫起始周)、2、4周双侧肱三头肌注射蛋白疫苗、佐剂和磷酸盐缓冲液(PBS).末次免疫后2周,酶联免疫吸附试验(ELISA)检测恒河猴血清中沙眼衣原体特异性IgG抗体和干扰素γ(IFN-γ),以及阴道冲洗液中沙眼衣原体特异性sIgA抗体,MTT法检测恒河猴淋巴细胞特异性增殖反应,观察恒河猴的迟发型超敏反应,以及恒河猴血清抗体中和试验,同源攻击后免疫荧光检测衣原体.用SPSS 14.0软件进行单向方差分析和LSD法进行组间多重比较.[结果]佐剂蛋白组恒河猴血清衣原体特异性IgG抗体A405值为1.718±0.213,淋巴细胞增殖指数为7.012±1.026,IFN-γ水平为(1086.000±121.730) ng/L,迟发型超敏反应皮丘直径为(11.000±2.134) mm;佐剂组分别为0.841±0.315、4.473±1.850、(409.000±53.440)ng/L、(3.000±0.914) mm;对照组分别为0.791±0.437、1.426±1.104、(162.000±48.046) ng/L、0.佐剂蛋白组各项指标与佐剂组和对照组比较,差异均有统计学意义.提示蛋白疫苗能诱导对沙眼衣原体血清E型株的特异性免疫应答.同源攻击后佐剂蛋白组衣原体持续阳性5周后转阴性,佐剂组及对照组持续阳性.[结论]沙眼衣原体rMOMP能刺激恒河猴产生特异性的体液和细胞免疫应答,能诱导恒河猴产生抗沙眼衣原体保护效应.
[目的]探討E型重組主外膜蛋白(rMOMP)對恆河猴誘導產生的特異性免疫應答效應.[方法]恆河猴分3組,每組2隻,分彆為佐劑蛋白組、佐劑組、對照組.各組均于第0(免疫起始週)、2、4週雙側肱三頭肌註射蛋白疫苗、佐劑和燐痠鹽緩遲液(PBS).末次免疫後2週,酶聯免疫吸附試驗(ELISA)檢測恆河猴血清中沙眼衣原體特異性IgG抗體和榦擾素γ(IFN-γ),以及陰道遲洗液中沙眼衣原體特異性sIgA抗體,MTT法檢測恆河猴淋巴細胞特異性增殖反應,觀察恆河猴的遲髮型超敏反應,以及恆河猴血清抗體中和試驗,同源攻擊後免疫熒光檢測衣原體.用SPSS 14.0軟件進行單嚮方差分析和LSD法進行組間多重比較.[結果]佐劑蛋白組恆河猴血清衣原體特異性IgG抗體A405值為1.718±0.213,淋巴細胞增殖指數為7.012±1.026,IFN-γ水平為(1086.000±121.730) ng/L,遲髮型超敏反應皮丘直徑為(11.000±2.134) mm;佐劑組分彆為0.841±0.315、4.473±1.850、(409.000±53.440)ng/L、(3.000±0.914) mm;對照組分彆為0.791±0.437、1.426±1.104、(162.000±48.046) ng/L、0.佐劑蛋白組各項指標與佐劑組和對照組比較,差異均有統計學意義.提示蛋白疫苗能誘導對沙眼衣原體血清E型株的特異性免疫應答.同源攻擊後佐劑蛋白組衣原體持續暘性5週後轉陰性,佐劑組及對照組持續暘性.[結論]沙眼衣原體rMOMP能刺激恆河猴產生特異性的體液和細胞免疫應答,能誘導恆河猴產生抗沙眼衣原體保護效應.
[목적]탐토E형중조주외막단백(rMOMP)대항하후유도산생적특이성면역응답효응.[방법]항하후분3조,매조2지,분별위좌제단백조、좌제조、대조조.각조균우제0(면역기시주)、2、4주쌍측굉삼두기주사단백역묘、좌제화린산염완충액(PBS).말차면역후2주,매련면역흡부시험(ELISA)검측항하후혈청중사안의원체특이성IgG항체화간우소γ(IFN-γ),이급음도충세액중사안의원체특이성sIgA항체,MTT법검측항하후림파세포특이성증식반응,관찰항하후적지발형초민반응,이급항하후혈청항체중화시험,동원공격후면역형광검측의원체.용SPSS 14.0연건진행단향방차분석화LSD법진행조간다중비교.[결과]좌제단백조항하후혈청의원체특이성IgG항체A405치위1.718±0.213,림파세포증식지수위7.012±1.026,IFN-γ수평위(1086.000±121.730) ng/L,지발형초민반응피구직경위(11.000±2.134) mm;좌제조분별위0.841±0.315、4.473±1.850、(409.000±53.440)ng/L、(3.000±0.914) mm;대조조분별위0.791±0.437、1.426±1.104、(162.000±48.046) ng/L、0.좌제단백조각항지표여좌제조화대조조비교,차이균유통계학의의.제시단백역묘능유도대사안의원체혈청E형주적특이성면역응답.동원공격후좌제단백조의원체지속양성5주후전음성,좌제조급대조조지속양성.[결론]사안의원체rMOMP능자격항하후산생특이성적체액화세포면역응답,능유도항하후산생항사안의원체보호효응.
[Objective] To observe the specific immune responses induced by the recombinant major outer membrane protein (rMOMP) vaccine against Chlamydia trachomatis E serotype in rhesus monkeys.[Methods] Six rhesus monkeys were equally divided into three groups:adjuvant and protein group vaccinated with purified rMOMP and Freund's adjuvants,adjuvant group immunized with Freund's adjuvants only,and control group immunized with phosphate buffer.All the rhesus monkeys were intramuscularly immunized in the triceps brachii for 3 times at a 2-week interval.Two weeks after the last vaccination,serum,vaginal wash and venous blood samples were collected from the rhesus monkeys,and lymphocytes were isolated from the blood samples.Enzyme linked immunosorbent assay (ELISA) was performed to determine the specific IgG antibody and interferon level in sera and secretory IgA (sIgA) level in wash samples,and methyl thiazolyl tetrazolium (MTT) assay to evaluate the proliferation of lymphocytes after stimulation with Chlaraydia trachomatis serotype E elementary bodies.Delayed hypersensitivity was observed in rhesus monkeys challenged by inactivated Chlamydia trachomatis serotype E elementary bodies.In vitro antibody neutralization assay was conducted with the serum from rhesus monkeys.Indirect immunofluorescenee was used to detect Chlamydia trachomatis in exfoliative vaginal cells from rhesus monkeys from week 1 to 10 after challenge with Chlamydia trachomatis.Data were statistically analyzed by using one-way analysis of variance and least significant difference (LSD) test with the SPSS 14.0 software.[Results] The adjuvant and protein group differed statistically from the adjuvant group and control group in the serum level of specific IgG antibody (1.718 ± 0.213 vs.0.841 ± 0.315 and 0.791 ±0.437,both P< 0.05),interferon ((1086 ± 121.730) ng/L vs.(409 + 53.440) ng/L and (162 ± 48.046) ng/L,both P< 0.05),lymphocyte proliferation index (7.012 ± 1.026 vs.4.473 ± 1.850 and 1A26 ± 1.104,both P<0.01 ) and the diameter of nodus in delayed hypersensitivity assay ( ( 1 1 ± 2.134) mm vs.(3 ± 0.914) mm and 0,both P < 0.01 ).After attack,the exfoliative cells kept positive for Chlamydia trachomatis in the adjuvant and protein group from week 1 to 5,and in the other 2 groups from week 1 to 10,but were negative in the adjuvant and protein group from week 6 to 10.[Conclusion] The rMOMP vaccine can induce a specific,protective,humoral and cellular immune response against Chlamydia tracbomatis in rhesus monkeys.