CAJ | 학술논문

目的研究胚胎干细胞动物体内抗Lewis非小细胞肺癌的作用及初步机制.方法以小鼠Lewis肺癌模型为基础,检测实验组和对照组小鼠的肿瘤生长情况和免疫指标,探讨胚胎干细胞在体内的抗肿瘤作用及其初步分子免疫机制.结果肿瘤生长曲线显示,与对照组小鼠相比,实验组小鼠肿瘤的生长速度明显减慢,从实验的第5天开始两组小鼠的肿瘤体积相比差异均有统计学意义(P＜0.05).小鼠外周血T、B淋巴细胞亚群检测结果,与对照组小鼠相比,实验组小鼠外周血CD4+、CD8+淋巴细胞比例增加,差异有统计学意义,分别为P＜0.05、P＜0.01,CD19+淋巴细胞比例下降(P＜0.05).血清细胞因子(IL-2、IL-4和IFN-γ)检测结果,与对照组相比,实验组小鼠外周血中IL-2和IFN-γ细胞因子水平增高,差异有统计学意义(P＜0.05),IL-4略有增高但无统计学意义.结论胚胎干细胞不仅在体外具有明显的抗肿瘤作用,在动物体内胚胎干细胞也能通过激活免疫系统从而产生有效的抗肿瘤作用,抑制或延缓肿瘤的形成和发展.
목적 연구배태간세포동물체내항Lewis비소세포폐암적작용급초보궤제.방법 이소서Lewis폐암모형위기출,검측실험조화대조조소서적종류생장정황화면역지표,탐토배태간세포재체내적항종류작용급기초보분자면역궤제.결과 종류생장곡선현시,여대조조소서상비,실험조소서종류적생장속도명현감만,종실험적제5천개시량조소서적종류체적상비차이균유통계학의의(P＜0.05).소서외주혈T、B림파세포아군검측결과,여대조조소서상비,실험조소서외주혈CD4+、CD8+림파세포비례증가,차이유통계학의의,분별위P＜0.05、P＜0.01,CD19+림파세포비례하강(P＜0.05).혈청세포인자(IL-2、IL-4화IFN-γ)검측결과,여대조조상비,실험조소서외주혈중IL-2화IFN-γ세포인자수평증고,차이유통계학의의(P＜0.05),IL-4략유증고단무통계학의의.결론 배태간세포불부재체외구유명현적항종류작용,재동물체내배태간세포야능통과격활면역계통종이산생유효적항종류작용,억제혹연완종류적형성화발전.
Objective To investigate the function and mechanism of embryonic stem cells against Lewis non-small cell lung cancer in vivo. Methods Based on the mouse Lewis non-small cell lung cancer model, we have tested some tumor growth indexes and investigated the immune response of embryonic stem cells against cancer cells. Results Compared with the mice in control group, mice in experimental group received obvious antitumor immunity, which means more activated lymphocytes and antitumor cytokines, resulted in the effective control and inhibition of tumor development. Conclusion Besides the antitumor effect in vitro, embryonic stem cells can also generates immune response in vivo, which could effectively inhibit and/or delay the development of cancer.