CAJ | 학술논문

目的探讨环氧化酶-2(COX-2)、骨桥蛋白(OPN)在人脑膜瘤中的表达及临床意义.方法选择脑膜瘤患者40例作为观察组,10例健康体检者作为对照组.采用ELISA法测定COX-2和OPN的浓度；采用免疫组化技术检测术后40例脑膜瘤组织及10例正常脑膜组织,COX-2、OPN及基质金属蛋白酶-9(MMP-9)的表达.结果侵袭组中COX-2、OPN的血清含量高于非侵袭组,差异有统计学意义(P＜0.01)；同样COX-2、OPN及MMP-9蛋白在侵袭组脑膜瘤中的表达均高于非侵袭组(P＜0.01).在侵袭性脑膜瘤中COX-2、OPN和MMP-9两两比较,其表达均具有显著相关性.结论 COX-2、OPN与脑膜瘤的侵袭性密切相关,一定程度上可作为评价脑膜瘤侵袭性及预后的辅助指标,通过术前血清学检测、术后免疫组化检查可判断脑膜瘤的潜在侵袭性,指导临床治疗方案的制定,提高疗效水平.
목적 탐토배양화매-2(COX-2)、골교단백(OPN)재인뇌막류중적표체급림상의의.방법 선택뇌막류환자40례작위관찰조,10례건강체검자작위대조조.채용ELISA법측정COX-2화OPN적농도；채용면역조화기술검측술후40례뇌막류조직급10례정상뇌막조직,COX-2、OPN급기질금속단백매-9(MMP-9)적표체.결과 침습조중COX-2、OPN적혈청함량고우비침습조,차이유통계학의의(P＜0.01)；동양COX-2、OPN급MMP-9단백재침습조뇌막류중적표체균고우비침습조(P＜0.01).재침습성뇌막류중COX-2、OPN화MMP-9량량비교,기표체균구유현저상관성.결론 COX-2、OPN여뇌막류적침습성밀절상관,일정정도상가작위평개뇌막류침습성급예후적보조지표,통과술전혈청학검측、술후면역조화검사가판단뇌막류적잠재침습성,지도림상치료방안적제정,제고료효수평.
Objective To investigate the expression of cyclooxygenase-2 (COX-2) and osteopontin( OPN ) in meningioma and the clinical significance.Methods Forty cases of meningioma were choosed as observation group,and 10 cases of health people were choosed as control group.The serum concentrations of COX-2 and OPN were detected by enzyme linked immunosorbent assay.The expression levels of OPN,COX-2 and MMP-9 were detected by immunohistochemical S-P method in 40 cases of meningiomas and 10 cases normal brain tissue.Results The serum concertrations of OPN and COX-2 in invasive meningioma were higher than those in non-invasive ( P ＜ O.O1 ).The expression levels of COX2,OPN and MMP-9 in invasive meningioma was higher than that in non-invasive meningioma (P ＜0.01 ).There were significant relationps between COX-2,OPN and MMP-9 in invasive meningioma.Conclusions COX-2,OPN are closely correlated with meningioma invasiveness,indicating that COX-2,OPN can evaluate invasiveness and prognostic of meningioma.Through detecting of COX-2,OPN in the samples of meningioma,we can judge the potential invasiveness of meningioma,and institute a right treatment plan to improve the curative effect of tumor patients.