CAJ | 학술논문

以耐光抑制的水稻(Oryza sativa L.)粳亚种品种"武育粳"和对光抑制敏感的籼亚种品种"汕优63"的剑叶为材料,比较研究了高光胁迫对水稻两个亚种光合系统的影响.结果表明,高光胁迫影响激发能在两个光系统之间的分配,抑制激发能向PSⅡ传递.在籼稻中激发峰和发射峰以及相关比值在受到高光胁迫后下降的幅度均比在粳稻中显著.与粳稻相比,高光胁迫几乎使籼稻的PSⅡ捕光色素蛋白复合体的三聚体解体,使其PSⅡ捕光色素蛋白复合体的二聚体、PSⅡ内周天线色素蛋白复合体、PSⅠ捕光色素蛋白复合体以及PSⅠ反应中心叶绿素a蛋白复合体的含量明显降低.高光胁迫导致水稻两亚种的PSⅡ捕光色素蛋白复合物单体含量的增加.类囊体膜多肽分析表明,高光胁迫使属于PSⅡ捕光色素蛋白复合体的27、25 kD蛋白和PSⅠ捕光色素蛋白复合体的21 kD蛋白含量在水稻两亚种中都降低,其中以25 kD蛋白含量降幅最大.在籼稻中高光胁迫使属于PSⅡ内周天线色素蛋白复合体的43 kD和47kD蛋白以及外周23kD蛋白含量明显降低而在粳稻中则影响较小.
이내광억제적수도(Oryza sativa L.)갱아충품충"무육갱"화대광억제민감적선아충품충"산우63"적검협위재료,비교연구료고광협박대수도량개아충광합계통적영향.결과표명,고광협박영향격발능재량개광계통지간적분배,억제격발능향PSⅡ전체.재선도중격발봉화발사봉이급상관비치재수도고광협박후하강적폭도균비재갱도중현저.여갱도상비,고광협박궤호사선도적PSⅡ포광색소단백복합체적삼취체해체,사기PSⅡ포광색소단백복합체적이취체、PSⅡ내주천선색소단백복합체、PSⅠ포광색소단백복합체이급PSⅠ반응중심협록소a단백복합체적함량명현강저.고광협박도치수도량아충적PSⅡ포광색소단백복합물단체함량적증가.류낭체막다태분석표명,고광협박사속우PSⅡ포광색소단백복합체적27、25 kD단백화PSⅠ포광색소단백복합체적21 kD단백함량재수도량아충중도강저,기중이25 kD단백함량강폭최대.재선도중고광협박사속우PSⅡ내주천선색소단백복합체적43 kD화47kD단백이급외주23kD단백함량명현강저이재갱도중칙영향교소.
Influence of high light stress on the photosynthesis of flag leaves of indica subspecies (cv."Shanyou 63", sensitive to photoinhibition) and japonica subspecies (cv. "Wuyujing", resistant to photoinhi-bition) of rice (Oryza sativa L.) was comparatively investigated. In both cultivars of rice, the excitation ener-gy distribution between two photosystems was altered and the excitation energy transfer from light harvestingchlorophyll protein complexes to PS Ⅱ was inhibited by high light stress. These decreases were more pro-nounced in indica rice cultivar as compared to japonica one. The analysis of mild SDS-PAGE showed that inindica rice, high light stress almost disaggregated the trimer of light harvesting chlorophyll protein complexes ofPS Ⅱ (LHC Ⅱ 1). The stress reduced the contents of internal antennae chlorophyll protein complexes of PS Ⅱ(CPa), light harvesting chlorophyll protein of PS Ⅰ (CPⅠ a) and Chl a protein complex of PS Ⅰ reaction cen-ter (CPⅠ) as well as dimer of LHC Ⅱ (LHC Ⅱ 2) in indica rice. In japonica subspecies, however, high lightstress depressed the contents of LHC Ⅱ 1, CPa and CPⅠ a, but slightly impacted on CPⅠ content. Moreover,the increase in the contents of monomer of LHC Ⅱ by high light stress was found in both subspecies. In consis-tent with above results, analysis of polypeptide indicated that the amounts of 27 kD and 25 kD polypeptide ofLHC Ⅱ in particular, as well as that of 21 kD polypeptide of CPⅠ a were reduced by high light stress in bothsubspecies. It was found that, comparing with japonica rice, the stress pronouncedly diminished 43 kD and 47kD proteins of CPa and 23 kD extrisic protein in indica rice.